LOCUS       sequence-250509-        8967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of B2M L13Sfs*31.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-B2M-L13Sfs*31
FEATURES             Location/Qualifiers
     source          1..8967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..567
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(159..176)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     386..405
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(579..581,582..584,585..1298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(579..581,582..584,585..1295)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(624..645)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(885..904)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1232..1253
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1312..1900
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1365..1385)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1783..1794)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1975..2208
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2327..2345)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2413..2517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2518..2586,2587..3378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2736..2755)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3549..4137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4038..4057
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4291..4308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4548..4569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4584..4589,4590..4607,4608..4614)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4622..4638
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4627..4649
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4646..4662
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4646..4662
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4712..4731
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4923..5556
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5603..5728
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6225..6458
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6643..6687
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6980..7097
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7145..8323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7376..8314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8271..8291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    8343..8367
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8729..8753)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8761..8779
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8804..8823)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8805..8823)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(8829..8849)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             8903..8944
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(8965..8967,1..565)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
       61 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
      121 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
      181 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
      241 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
      301 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
      361 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
      421 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
      481 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
      541 tttcctttga aaaacacgat gataatatgg ccacacatat ggtgagcaag ggcgaggagc
      601 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      661 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      721 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      781 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      841 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      901 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      961 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1021 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1081 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1141 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1201 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1261 ccgggatcac tctcggcatg gacgagctgt acaagtaaat cgatagatcc taatcaacct
     1321 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1381 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1441 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     1501 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     1561 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     1621 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     1681 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     1741 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     1801 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     1861 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     1921 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     1981 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     2041 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2101 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2161 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     2221 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     2281 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     2341 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2401 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2461 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2521 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2581 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2641 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2701 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2761 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2821 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2881 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2941 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3001 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3061 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3121 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3181 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3241 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3301 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3361 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3421 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3481 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3541 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3601 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3661 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3721 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3781 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3841 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3901 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3961 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4021 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4081 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4141 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4201 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4261 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4321 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
     4381 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4441 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
     4501 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4561 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4621 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
     4681 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4741 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     4801 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     4861 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     4921 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     4981 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     5041 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     5101 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     5161 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     5221 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     5281 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     5341 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     5401 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5461 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5521 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     5581 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     5641 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     5701 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     5761 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     5821 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     5881 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     5941 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     6001 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     6061 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     6121 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     6181 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     6241 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     6301 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6361 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     6421 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6481 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     6541 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     6601 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     6661 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     6721 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     6781 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     6841 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     6901 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa
     6961 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     7021 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     7081 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggactagtc
     7141 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     7201 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     7261 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     7321 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     7381 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     7441 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     7501 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     7561 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     7621 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     7681 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     7741 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     7801 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     7861 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     7921 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     7981 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     8041 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     8101 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     8161 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     8221 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     8281 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaagcggcc ctgcagatat
     8341 caacaagttt gtacaaaaaa gcaggcacca tgtctcgctc cgtggcctta gctgtgctcg
     8401 cgctactctc tttctggcct ggaggctatc cagcgtactc caaagattca ggtttactca
     8461 cgtcatccag cagagaatgg aaagtcaaat ttcctgaatt gctatgtgtc tgggtttcat
     8521 ccatccgaca ttgaagttga cttactgaag aatggagaga gaattgaaaa agtggagcat
     8581 tcagacttgt ctttcagcaa ggactggtct ttctatctct tgtactacac tgaattcacc
     8641 cccactgaaa aagatgagta tgcctgccgt gtgaaccatg tgactttgtc acagcccaag
     8701 atagttaagt gggatcgaga catgtagaac ccagctttct tgtacaaagt ggtgatatcc
     8761 aattaaccct cactaaaggg tgtgttattt gtttgtaaag tatccctata gtgagtcgta
     8821 ttaccaccca acttttctat acaaagtggt tgatatccag cacagtggcg gccgctcgag
     8881 tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc
     8941 tacgcgtacc ggttagtaat gagatcc
//
