LOCUS       sequence-250511-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR E709K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-E709K
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    293..410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        458..1636
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          689..1627
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1584..1604
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1656..1680
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1683..1754,1755..3617,3618..3686,3687..5315)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKKTEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5317..5341)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5349..5367
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5392..5411)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5393..5411)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5417..5437)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5491..5532
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5553..6120
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5570..6122
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5714..5731)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5941..5960
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6134..6733
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6134..6153)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6630..6650
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6747..7335
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6800..6820)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7218..7229)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7410..7643
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7762..7780)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7848..7952
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7953..8021,8022..8813)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8984..9572
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9473..9492
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9726..9743
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9983..10004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10019..10024,10025..10042,10043..10049)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10057..10073
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10062..10084
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10081..10097
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10081..10097
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10147..10166
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10358..10991
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11038..11163
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11660..11893
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12078..12122
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
       61 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
      121 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
      181 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
      241 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa
      301 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      361 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      421 acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc
      481 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      541 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      601 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      661 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      721 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      781 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      841 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      901 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      961 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     1021 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     1081 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     1141 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     1201 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     1261 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1321 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1381 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1441 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1501 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1561 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1621 atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa aaagcaggca
     1681 ccatgcgacc ctccgggacg gccggggcag cgctcctggc gctgctggct gcgctctgcc
     1741 cggcgagtcg ggctctggag gaaaagaaag tttgccaagg cacgagtaac aagctcacgc
     1801 agttgggcac ttttgaagat cattttctca gcctccagag gatgttcaat aactgtgagg
     1861 tggtccttgg gaatttggaa attacctatg tgcagaggaa ttatgatctt tccttcttaa
     1921 agaccatcca ggaggtggct ggttatgtcc tcattgccct caacacagtg gagcgaattc
     1981 ctttggaaaa cctgcagatc atcagaggaa atatgtacta cgaaaattcc tatgccttag
     2041 cagtcttatc taactatgat gcaaataaaa ccggactgaa ggagctgccc atgagaaatt
     2101 tacaggaaat cctgcatggc gccgtgcggt tcagcaacaa ccctgccctg tgcaacgtgg
     2161 agagcatcca gtggcgggac atagtcagca gtgactttct cagcaacatg tcgatggact
     2221 tccagaacca cctgggcagc tgccaaaagt gtgatccaag ctgtcccaat gggagctgct
     2281 ggggtgcagg agaggagaac tgccagaaac tgaccaaaat catctgtgcc cagcagtgct
     2341 ccgggcgctg ccgtggcaag tcccccagtg actgctgcca caaccagtgt gctgcaggct
     2401 gcacaggccc ccgggagagc gactgcctgg tctgccgcaa attccgagac gaagccacgt
     2461 gcaaggacac ctgcccccca ctcatgctct acaaccccac cacgtaccag atggatgtga
     2521 accccgaggg caaatacagc tttggtgcca cctgcgtgaa gaagtgtccc cgtaattatg
     2581 tggtgacaga tcacggctcg tgcgtccgag cctgtggggc cgacagctat gagatggagg
     2641 aagacggcgt ccgcaagtgt aagaagtgcg aagggccttg ccgcaaagtg tgtaacggaa
     2701 taggtattgg tgaatttaaa gactcactct ccataaatgc tacgaatatt aaacacttca
     2761 aaaactgcac ctccatcagt ggcgatctcc acatcctgcc ggtggcattt aggggtgact
     2821 ccttcacaca tactcctcct ctggatccac aggaactgga tattctgaaa accgtaaagg
     2881 aaatcacagg gtttttgctg attcaggctt ggcctgaaaa caggacggac ctccatgcct
     2941 ttgagaacct agaaatcata cgcggcagga ccaagcaaca tggtcagttt tctcttgcag
     3001 tcgtcagcct gaacataaca tccttgggat tacgctccct caaggagata agtgatggag
     3061 atgtgataat ttcaggaaac aaaaatttgt gctatgcaaa tacaataaac tggaaaaaac
     3121 tgtttgggac ctccggtcag aaaaccaaaa ttataagcaa cagaggtgaa aacagctgca
     3181 aggccacagg ccaggtctgc catgccttgt gctcccccga gggctgctgg ggcccggagc
     3241 ccagggactg cgtctcttgc cggaatgtca gccgaggcag ggaatgcgtg gacaagtgca
     3301 accttctgga gggtgagcca agggagtttg tggagaactc tgagtgcata cagtgccacc
     3361 cagagtgcct gcctcaggcc atgaacatca cctgcacagg acggggacca gacaactgta
     3421 tccagtgtgc ccactacatt gacggccccc actgcgtcaa gacctgcccg gcaggagtca
     3481 tgggagaaaa caacaccctg gtctggaagt acgcagacgc cggccatgtg tgccacctgt
     3541 gccatccaaa ctgcacctac ggatgcactg ggccaggtct tgaaggctgt ccaacgaatg
     3601 ggcctaagat cccgtccatc gccactggga tggtgggggc cctcctcttg ctgctggtgg
     3661 tggccctggg gatcggcctc ttcatgcgaa ggcgccacat cgttcggaag cgcacgctgc
     3721 ggaggctgct gcaggagagg gagcttgtgg agcctcttac acccagtgga gaagctccca
     3781 accaagctct cttgaggatc ttgaagaaaa ctgaattcaa aaagatcaaa gtgctgggct
     3841 ccggtgcgtt cggcacggtg tataagggac tctggatccc agaaggtgag aaagttaaaa
     3901 ttcccgtcgc tatcaaggaa ttaagagaag caacatctcc gaaagccaac aaggaaatcc
     3961 tcgatgaagc ctacgtgatg gccagcgtgg acaaccccca cgtgtgccgc ctgctgggca
     4021 tctgcctcac ctccaccgtg cagctcatca cgcagctcat gcccttcggc tgcctcctgg
     4081 actatgtccg ggaacacaaa gacaatattg gctcccagta cctgctcaac tggtgtgtgc
     4141 agatcgcaaa gggcatgaac tacttggagg accgtcgctt ggtgcaccgc gacctggcag
     4201 ccaggaacgt actggtgaaa acaccgcagc atgtcaagat cacagatttt gggctggcca
     4261 aactgctggg tgcggaagag aaagaatacc atgcagaagg aggcaaagtg cctatcaagt
     4321 ggatggcatt ggaatcaatt ttacacagaa tctataccca ccagagtgat gtctggagct
     4381 acggggtgac cgtttgggag ttgatgacct ttggatccaa gccatatgac ggaatccctg
     4441 ccagcgagat ctcctccatc ctggagaaag gagaacgcct ccctcagcca cccatatgta
     4501 ccatcgatgt ctacatgatc atggtcaagt gctggatgat agacgcagat agtcgcccaa
     4561 agttccgtga gttgatcatc gaattctcca aaatggcccg agacccccag cgctaccttg
     4621 tcattcaggg ggatgaaaga atgcatttgc caagtcctac agactccaac ttctaccgtg
     4681 ccctgatgga tgaagaagac atggacgacg tggtggatgc cgacgagtac ctcatcccac
     4741 agcagggctt cttcagcagc ccctccacgt cacggactcc cctcctgagc tctctgagtg
     4801 caaccagcaa caattccacc gtggcttgca ttgatagaaa tgggctgcaa agctgtccca
     4861 tcaaggaaga cagcttcttg cagcgataca gctcagaccc cacaggcgcc ttgactgagg
     4921 acagcataga cgacaccttc ctcccagtgc ctgaatacat aaaccagtcc gttcccaaaa
     4981 ggcccgctgg ctctgtgcag aatcctgtct atcacaatca gcctctgaac cccgcgccca
     5041 gcagagaccc acactaccag gacccccaca gcactgcagt gggcaacccc gagtatctca
     5101 acactgtcca gcccacctgt gtcaacagca cattcgacag ccctgcccac tgggcccaga
     5161 aaggcagcca ccaaattagc ctggacaacc ctgactacca gcaggacttc tttcccaagg
     5221 aagccaagcc aaatggcatc tttaagggct ccacagctga aaatgcagaa tacctaaggg
     5281 tcgcgccaca aagcagtgaa tttattggag catagaaccc agctttcttg tacaaagtgg
     5341 tgatatccaa ttaaccctca ctaaaggtga tatagtagtg aagaaataag tccctatagt
     5401 gagtcgtatt accacccaac ttttctatac aaagtggttg atatccagca cagtggcggc
     5461 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
     5521 ctcgattcta cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc
     5581 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     5641 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     5701 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     5761 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     5821 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     5881 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     5941 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6001 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6061 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa
     6121 tatggccaca catatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     6181 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     6241 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     6301 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     6361 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     6421 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     6481 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     6541 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     6601 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     6661 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     6721 gcccggtgcc taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat
     6781 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6841 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6901 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6961 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     7021 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7081 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7141 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7201 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7261 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7321 gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct
     7381 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7441 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7501 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7561 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7621 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     7681 acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag
     7741 atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     7801 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7861 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7921 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7981 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     8041 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     8101 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     8161 ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     8221 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     8281 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     8341 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     8401 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     8461 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     8521 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8581 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8641 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8701 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8761 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8821 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8881 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8941 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     9001 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     9061 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     9121 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     9181 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     9241 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     9301 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     9361 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     9421 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     9481 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     9541 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     9601 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9661 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9721 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     9781 ccgcgcgttg gccgattcat taatgcagca agctcatggc tgactaattt tttttattta
     9841 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     9901 tggaggccta ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag
     9961 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    10021 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    10081 caggaaacag ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt
    10141 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
    10201 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
    10261 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
    10321 attgtatttg ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa
    10381 agaagacaag atatccttga tctgtggatc taccacacac aaggctactt ccctgattag
    10441 cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag
    10501 ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg
    10561 ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg
    10621 tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag
    10681 aactgctgat atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt
    10741 ggcctgggcg ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt
    10801 tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
    10861 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
    10921 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
    10981 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
    11041 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
    11101 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
    11161 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
    11221 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
    11281 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
    11341 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
    11401 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
    11461 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca
    11521 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
    11581 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
    11641 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
    11701 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
    11761 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
    11821 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
    11881 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
    11941 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
    12001 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
    12061 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
    12121 tg
//
