LOCUS       sequence-250515-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR E709Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-E709Q
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    218..335
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        383..1561
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          614..1552
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1509..1529
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1581..1605
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1608..1679,1680..3542,3543..3611,3612..5240)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKQTEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5242..5266)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5274..5292
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5317..5336)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5318..5336)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5342..5362)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5416..5457
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5478..6045
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5495..6047
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5639..5656)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5866..5885
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6059..6658
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6059..6078)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6555..6575
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6672..7260
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6725..6745)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7143..7154)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7335..7568
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7687..7705)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7773..7877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7878..7946,7947..8738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8909..9497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9398..9417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9651..9668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9908..9929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9944..9949,9950..9967,9968..9974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9982..9998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9987..10009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10006..10022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10006..10022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10072..10091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10283..10916
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10963..11088
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11585..11818
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12003..12047
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
       61 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
      121 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
      181 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      241 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      301 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      361 atccagtttg gactagtcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      421 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
      481 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
      541 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
      601 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
      661 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
      721 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
      781 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
      841 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
      901 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
      961 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     1021 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     1081 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     1141 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     1201 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1261 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1321 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1381 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1441 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1501 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     1561 aagcggccct gcagatatca acaagtttgt acaaaaaagc aggcaccatg cgaccctccg
     1621 ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc
     1681 tggaggaaaa gaaagtttgc caaggcacga gtaacaagct cacgcagttg ggcacttttg
     1741 aagatcattt tctcagcctc cagaggatgt tcaataactg tgaggtggtc cttgggaatt
     1801 tggaaattac ctatgtgcag aggaattatg atctttcctt cttaaagacc atccaggagg
     1861 tggctggtta tgtcctcatt gccctcaaca cagtggagcg aattcctttg gaaaacctgc
     1921 agatcatcag aggaaatatg tactacgaaa attcctatgc cttagcagtc ttatctaact
     1981 atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag aaatttacag gaaatcctgc
     2041 atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc atccagtggc
     2101 gggacatagt cagcagtgac tttctcagca acatgtcgat ggacttccag aaccacctgg
     2161 gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt gcaggagagg
     2221 agaactgcca gaaactgacc aaaatcatct gtgcccagca gtgctccggg cgctgccgtg
     2281 gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc aggctgcaca ggcccccggg
     2341 agagcgactg cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc
     2401 ccccactcat gctctacaac cccaccacgt accagatgga tgtgaacccc gagggcaaat
     2461 acagctttgg tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg
     2521 gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat ggaggaagac ggcgtccgca
     2581 agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt attggtgaat
     2641 ttaaagactc actctccata aatgctacga atattaaaca cttcaaaaac tgcacctcca
     2701 tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc acacatactc
     2761 ctcctctgga tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc acagggtttt
     2821 tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag aacctagaaa
     2881 tcatacgcgg caggaccaag caacatggtc agttttctct tgcagtcgtc agcctgaaca
     2941 taacatcctt gggattacgc tccctcaagg agataagtga tggagatgtg ataatttcag
     3001 gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt gggacctccg
     3061 gtcagaaaac caaaattata agcaacagag gtgaaaacag ctgcaaggcc acaggccagg
     3121 tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg gactgcgtct
     3181 cttgccggaa tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg
     3241 agccaaggga gtttgtggag aactctgagt gcatacagtg ccacccagag tgcctgcctc
     3301 aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag tgtgcccact
     3361 acattgacgg cccccactgc gtcaagacct gcccggcagg agtcatggga gaaaacaaca
     3421 ccctggtctg gaagtacgca gacgccggcc atgtgtgcca cctgtgccat ccaaactgca
     3481 cctacggatg cactgggcca ggtcttgaag gctgtccaac gaatgggcct aagatcccgt
     3541 ccatcgccac tgggatggtg ggggccctcc tcttgctgct ggtggtggcc ctggggatcg
     3601 gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg
     3661 agagggagct tgtggagcct cttacaccca gtggagaagc tcccaaccaa gctctcttga
     3721 ggatcttgaa gcaaactgaa ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca
     3781 cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc gtcgctatca
     3841 aggaattaag agaagcaaca tctccgaaag ccaacaagga aatcctcgat gaagcctacg
     3901 tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc ctcacctcca
     3961 ccgtgcagct catcacgcag ctcatgccct tcggctgcct cctggactat gtccgggaac
     4021 acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc gcaaagggca
     4081 tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg aacgtactgg
     4141 tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaactg ctgggtgcgg
     4201 aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg gcattggaat
     4261 caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg gtgaccgttt
     4321 gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc gagatctcct
     4381 ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc gatgtctaca
     4441 tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc cgtgagttga
     4501 tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt cagggggatg
     4561 aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg atggatgaag
     4621 aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag ggcttcttca
     4681 gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc agcaacaatt
     4741 ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag gaagacagct
     4801 tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc atagacgaca
     4861 ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc gctggctctg
     4921 tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga gacccacact
     4981 accaggaccc ccacagcact gcagtgggca accccgagta tctcaacact gtccagccca
     5041 cctgtgtcaa cagcacattc gacagccctg cccactgggc ccagaaaggc agccaccaaa
     5101 ttagcctgga caaccctgac taccagcagg acttctttcc caaggaagcc aagccaaatg
     5161 gcatctttaa gggctccaca gctgaaaatg cagaatacct aagggtcgcg ccacaaagca
     5221 gtgaatttat tggagcatag aacccagctt tcttgtacaa agtggtgata tccaattaac
     5281 cctcactaaa ggattgattt gttgaaataa gtgttgccct atagtgagtc gtattaccac
     5341 ccaacttttc tatacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag
     5401 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     5461 accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5521 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5581 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5641 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5701 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5761 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5821 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     5881 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5941 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     6001 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat
     6061 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6121 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6181 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6241 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6301 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6361 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6421 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6481 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6541 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6601 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctaaat
     6661 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6721 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6781 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6841 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6901 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6961 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7021 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     7081 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7141 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7201 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7261 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7321 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7381 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7441 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7501 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7561 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7621 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
     7681 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7741 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7801 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7861 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7921 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7981 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     8041 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     8101 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     8161 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8221 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8281 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8341 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8401 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8461 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8521 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8581 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8641 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8701 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8761 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8821 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8881 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8941 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     9001 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     9061 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9121 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9181 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9241 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9301 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9361 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9421 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9481 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9541 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9601 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9661 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9721 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc
     9781 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9841 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9901 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9961 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    10021 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10081 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10141 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    10201 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10261 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    10321 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    10381 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    10441 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    10501 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    10561 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    10621 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    10681 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    10741 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    10801 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    10861 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    10921 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    10981 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    11041 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    11101 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    11161 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    11221 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    11281 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    11341 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    11401 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
    11461 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    11521 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    11581 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    11641 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    11701 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
    11761 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
    11821 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    11881 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
    11941 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
    12001 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
    12061 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
    12121 gt
//
