LOCUS       sequence-250518-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR E804G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-E804G
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    154..271
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        319..1497
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          550..1488
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1445..1465
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1517..1541
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1544..1615,1616..3478,3479..3547,3548..5176)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVRGHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5178..5202)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5210..5228
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5253..5272)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5254..5272)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5278..5298)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5352..5393
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5414..5981
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5431..5983
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5575..5592)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5802..5821
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5995..6594
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5995..6014)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6491..6511
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6608..7196
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6661..6681)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7079..7090)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7271..7504
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7623..7641)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7709..7813
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7814..7882,7883..8674)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8845..9433
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9334..9353
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9587..9604
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9844..9865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9880..9885,9886..9903,9904..9910)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9918..9934
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9923..9945
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9942..9958
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9942..9958
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10008..10027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10219..10852
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10899..11024
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11521..11754
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11939..11983
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
       61 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
      121 gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
      181 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
      241 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
      301 agtttggact agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
      361 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
      421 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
      481 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
      541 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
      601 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
      661 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
      721 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
      781 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
      841 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
      901 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
      961 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     1021 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     1081 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     1141 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     1201 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     1261 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     1321 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     1381 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     1441 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc
     1501 ggccctgcag atatcaacaa gtttgtacaa aaaagcaggc accatgcgac cctccgggac
     1561 ggccggggca gcgctcctgg cgctgctggc tgcgctctgc ccggcgagtc gggctctgga
     1621 ggaaaagaaa gtttgccaag gcacgagtaa caagctcacg cagttgggca cttttgaaga
     1681 tcattttctc agcctccaga ggatgttcaa taactgtgag gtggtccttg ggaatttgga
     1741 aattacctat gtgcagagga attatgatct ttccttctta aagaccatcc aggaggtggc
     1801 tggttatgtc ctcattgccc tcaacacagt ggagcgaatt cctttggaaa acctgcagat
     1861 catcagagga aatatgtact acgaaaattc ctatgcctta gcagtcttat ctaactatga
     1921 tgcaaataaa accggactga aggagctgcc catgagaaat ttacaggaaa tcctgcatgg
     1981 cgccgtgcgg ttcagcaaca accctgccct gtgcaacgtg gagagcatcc agtggcggga
     2041 catagtcagc agtgactttc tcagcaacat gtcgatggac ttccagaacc acctgggcag
     2101 ctgccaaaag tgtgatccaa gctgtcccaa tgggagctgc tggggtgcag gagaggagaa
     2161 ctgccagaaa ctgaccaaaa tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa
     2221 gtcccccagt gactgctgcc acaaccagtg tgctgcaggc tgcacaggcc cccgggagag
     2281 cgactgcctg gtctgccgca aattccgaga cgaagccacg tgcaaggaca cctgcccccc
     2341 actcatgctc tacaacccca ccacgtacca gatggatgtg aaccccgagg gcaaatacag
     2401 ctttggtgcc acctgcgtga agaagtgtcc ccgtaattat gtggtgacag atcacggctc
     2461 gtgcgtccga gcctgtgggg ccgacagcta tgagatggag gaagacggcg tccgcaagtg
     2521 taagaagtgc gaagggcctt gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa
     2581 agactcactc tccataaatg ctacgaatat taaacacttc aaaaactgca cctccatcag
     2641 tggcgatctc cacatcctgc cggtggcatt taggggtgac tccttcacac atactcctcc
     2701 tctggatcca caggaactgg atattctgaa aaccgtaaag gaaatcacag ggtttttgct
     2761 gattcaggct tggcctgaaa acaggacgga cctccatgcc tttgagaacc tagaaatcat
     2821 acgcggcagg accaagcaac atggtcagtt ttctcttgca gtcgtcagcc tgaacataac
     2881 atccttggga ttacgctccc tcaaggagat aagtgatgga gatgtgataa tttcaggaaa
     2941 caaaaatttg tgctatgcaa atacaataaa ctggaaaaaa ctgtttggga cctccggtca
     3001 gaaaaccaaa attataagca acagaggtga aaacagctgc aaggccacag gccaggtctg
     3061 ccatgccttg tgctcccccg agggctgctg gggcccggag cccagggact gcgtctcttg
     3121 ccggaatgtc agccgaggca gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc
     3181 aagggagttt gtggagaact ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc
     3241 catgaacatc acctgcacag gacggggacc agacaactgt atccagtgtg cccactacat
     3301 tgacggcccc cactgcgtca agacctgccc ggcaggagtc atgggagaaa acaacaccct
     3361 ggtctggaag tacgcagacg ccggccatgt gtgccacctg tgccatccaa actgcaccta
     3421 cggatgcact gggccaggtc ttgaaggctg tccaacgaat gggcctaaga tcccgtccat
     3481 cgccactggg atggtggggg ccctcctctt gctgctggtg gtggccctgg ggatcggcct
     3541 cttcatgcga aggcgccaca tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag
     3601 ggagcttgtg gagcctctta cacccagtgg agaagctccc aaccaagctc tcttgaggat
     3661 cttgaaggaa actgaattca aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt
     3721 gtataaggga ctctggatcc cagaaggtga gaaagttaaa attcccgtcg ctatcaagga
     3781 attaagagaa gcaacatctc cgaaagccaa caaggaaatc ctcgatgaag cctacgtgat
     3841 ggccagcgtg gacaaccccc acgtgtgccg cctgctgggc atctgcctca cctccaccgt
     3901 gcagctcatc acgcagctca tgcccttcgg ctgcctcctg gactatgtcc ggggacacaa
     3961 agacaatatt ggctcccagt acctgctcaa ctggtgtgtg cagatcgcaa agggcatgaa
     4021 ctacttggag gaccgtcgct tggtgcaccg cgacctggca gccaggaacg tactggtgaa
     4081 aacaccgcag catgtcaaga tcacagattt tgggctggcc aaactgctgg gtgcggaaga
     4141 gaaagaatac catgcagaag gaggcaaagt gcctatcaag tggatggcat tggaatcaat
     4201 tttacacaga atctataccc accagagtga tgtctggagc tacggggtga ccgtttggga
     4261 gttgatgacc tttggatcca agccatatga cggaatccct gccagcgaga tctcctccat
     4321 cctggagaaa ggagaacgcc tccctcagcc acccatatgt accatcgatg tctacatgat
     4381 catggtcaag tgctggatga tagacgcaga tagtcgccca aagttccgtg agttgatcat
     4441 cgaattctcc aaaatggccc gagaccccca gcgctacctt gtcattcagg gggatgaaag
     4501 aatgcatttg ccaagtccta cagactccaa cttctaccgt gccctgatgg atgaagaaga
     4561 catggacgac gtggtggatg ccgacgagta cctcatccca cagcagggct tcttcagcag
     4621 cccctccacg tcacggactc ccctcctgag ctctctgagt gcaaccagca acaattccac
     4681 cgtggcttgc attgatagaa atgggctgca aagctgtccc atcaaggaag acagcttctt
     4741 gcagcgatac agctcagacc ccacaggcgc cttgactgag gacagcatag acgacacctt
     4801 cctcccagtg cctgaataca taaaccagtc cgttcccaaa aggcccgctg gctctgtgca
     4861 gaatcctgtc tatcacaatc agcctctgaa ccccgcgccc agcagagacc cacactacca
     4921 ggacccccac agcactgcag tgggcaaccc cgagtatctc aacactgtcc agcccacctg
     4981 tgtcaacagc acattcgaca gccctgccca ctgggcccag aaaggcagcc accaaattag
     5041 cctggacaac cctgactacc agcaggactt ctttcccaag gaagccaagc caaatggcat
     5101 ctttaagggc tccacagctg aaaatgcaga atacctaagg gtcgcgccac aaagcagtga
     5161 atttattgga gcatagaacc cagctttctt gtacaaagtg gtgatatcca attaaccctc
     5221 actaaagggt ttagtgatta aagatagatt agccctatag tgagtcgtat taccacccaa
     5281 cttttctata caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     5341 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     5401 gttagtaatg agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     5461 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     5521 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     5581 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     5641 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     5701 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     5761 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     5821 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     5881 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     5941 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgacc
     6001 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     6061 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     6121 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     6181 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     6241 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     6301 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     6361 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     6421 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     6481 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     6541 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctaaatcgat
     6601 agatcctaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6661 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6721 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6781 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6841 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6901 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6961 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     7021 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     7081 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     7141 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga
     7201 gatcctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     7261 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     7321 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7381 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7441 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7501 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7561 tcagagagtg agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa
     7621 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     7681 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     7741 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     7801 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     7861 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     7921 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     7981 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8041 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     8101 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8161 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8221 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8281 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8341 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8401 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8461 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8521 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8581 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     8641 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     8701 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     8761 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     8821 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     8881 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     8941 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9001 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9061 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9121 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9181 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9241 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9301 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     9361 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     9421 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     9481 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     9541 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     9601 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     9661 ttaatgcagc aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9721 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9781 aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     9841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     9901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat
     9961 gattacgaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10021 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10081 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    10141 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    10201 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
    10261 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
    10321 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
    10381 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
    10441 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
    10501 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
    10561 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
    10621 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
    10681 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
    10741 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
    10801 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
    10861 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
    10921 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
    10981 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
    11041 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
    11101 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
    11161 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
    11221 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
    11281 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
    11341 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
    11401 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
    11461 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
    11521 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    11581 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    11641 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    11701 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    11761 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    11821 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    11881 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    11941 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    12001 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    12061 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
    12121 at
//
