LOCUS       sequence-250519-        9232 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BCL2 H20Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BCL2-H20Q
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(768..792)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             845..886
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    907..1474
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    924..1476
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1068..1085)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1295..1314
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1488..1490,1491..1493,1494..2207)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1488..1490,1491..1493,1494..2204)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1533..1554)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1794..1813)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2141..2162
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2221..2809
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2274..2294)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2692..2703)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2884..3117
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(3236..3254)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3322..3426
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3427..3495,3496..4287)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3645..3664)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4458..5046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4947..4966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5200..5217
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5457..5478
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5493..5498,5499..5516,5517..5523)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5531..5547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5536..5558
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5555..5571
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5555..5571
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5621..5640
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5832..6465
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6512..6637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7134..7367
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7552..7596
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7889..8006
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8054..9232
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8285..9223
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     9180..9200
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg cgcacgctgg
       61 gagaacgggg tacgataacc gggagatagt gatgaagtac atccaatata agctgtcgca
      121 gaggggctac gagtgggatg cgggagatgt gggcgccgcg cccccggggg ccgcccccgc
      181 accgggcatc ttctcctccc agcccgggca cacgccccat ccagccgcat cccgggaccc
      241 ggtcgccagg acctcgccgc tgcagacccc ggctgccccc ggcgccgccg cggggcctgc
      301 gctcagcccg gtgccacctg tggtccacct gaccctccgc caggccggcg acgacttctc
      361 ccgccgctac cgccgcgact tcgccgagat gtccagccag ctgcacctga cgcccttcac
      421 cgcgcgggga cgctttgcca cggtggtgga ggagctcttc agggacgggg tgaactgggg
      481 gaggattgtg gccttctttg agttcggtgg ggtcatgtgt gtggagagcg tcaaccggga
      541 gatgtcgccc ctggtggaca acatcgccct gtggatgact gagtacctga accggcacct
      601 gcacacctgg atccaggata acggaggctg ggatgccttt gtggaactgt acggccccag
      661 catgcggcct ctgtttgatt tctcctggct gtctctgaag actctgctca gtttggccct
      721 ggtgggagct tgcatcaccc tgggtgccta tctgggccac aagtagaacc cagctttctt
      781 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
      841 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta
      901 atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
      961 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     1021 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     1081 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     1141 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     1201 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     1261 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     1321 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     1381 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     1441 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg gtgagcaagg
     1501 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     1561 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     1621 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     1681 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     1741 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     1801 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     1861 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     1921 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     1981 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     2041 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     2101 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     2161 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaatc gatagatcct
     2221 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2281 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     2341 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     2401 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     2461 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     2521 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     2581 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     2641 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     2701 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     2761 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt
     2821 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     2881 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     2941 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3001 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3061 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     3121 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     3181 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
     3241 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     3301 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3361 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3421 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3481 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3541 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     3601 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3661 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3721 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     3781 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3841 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3901 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3961 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4021 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4081 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4141 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4201 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4261 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     4621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     4681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     4741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     4801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5041 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5101 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5161 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5221 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5281 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     5341 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     5401 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5461 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     5521 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
     5581 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5641 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     5701 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     5761 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     5821 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     5881 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     5941 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     6001 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     6061 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     6121 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     6181 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     6241 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     6301 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6361 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6421 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     6481 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     6541 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     6601 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     6661 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     6721 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     6781 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     6841 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     6901 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     6961 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     7021 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     7081 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     7141 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     7201 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     7261 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     7321 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     7381 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     7441 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     7501 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     7561 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     7621 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     7681 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     7741 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     7801 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga
     7861 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
     7921 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
     7981 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt
     8041 ggactagtcg tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     8101 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     8161 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     8221 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     8281 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     8341 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     8401 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     8461 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     8521 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     8581 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     8641 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     8701 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     8761 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     8821 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     8881 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     8941 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     9001 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     9061 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     9121 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     9181 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt ga
//
