LOCUS       sequence-250521-       11606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BMPR2 Q6*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11606)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BMPR2-Q6*
FEATURES             Location/Qualifiers
     source          1..11606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1226
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          279..1217
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1174..1194
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1246..1270
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4391..4415)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4423..4441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4466..4485)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4467..4485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4491..4511)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             4565..4606
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4627..5194
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4644..5196
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4788..4805)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5015..5034
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5208..5807
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5208..5227)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5704..5724
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5821..6409
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5874..5894)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6292..6303)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6484..6717
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6836..6854)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6922..7026
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7027..7095,7096..7887)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7245..7264)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8058..8646
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8547..8566
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8800..8817
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9057..9078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9093..9098,9099..9116,9117..9123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9131..9147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9136..9158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9155..9171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9155..9171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9221..9240
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9432..10065
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10112..10237
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10734..10967
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11152..11196
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11489..11606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc
       61 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      121 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      181 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      241 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      301 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      361 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      421 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      481 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      541 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      601 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      661 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      721 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      781 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      841 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
      901 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
      961 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1021 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1081 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1141 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1201 tttttcttcc atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa
     1261 aaagcaggca ccatgacttc ctcgctgtag cggccctggc gggtgccctg gctaccatgg
     1321 accatcctgc tggtcagcac tgcggctgct tcgcagaatc aagaacggct atgtgcgttt
     1381 aaagatccgt atcagcaaga ccttgggata ggtgagagta gaatctctca tgaaaatggg
     1441 acaatattat gctcgaaagg tagcacctgc tatggccttt gggagaaatc aaaaggggac
     1501 ataaatcttg taaaacaagg atgttggtct cacattggag atccccaaga gtgtcactat
     1561 gaagaatgtg tagtaactac cactcctccc tcaattcaga atggaacata ccgtttctgc
     1621 tgttgtagca cagatttatg taatgtcaac tttactgaga attttccacc tcctgacaca
     1681 acaccactca gtccacctca ttcatttaac cgagatgaga caataatcat tgctttggca
     1741 tcagtctctg tattagctgt tttgatagtt gccttatgct ttggatacag aatgttgaca
     1801 ggagaccgta aacaaggtct tcacagtatg aacatgatgg aggcagcagc atccgaaccc
     1861 tctcttgatc tagataatct gaaactgttg gagctgattg gccgaggtcg atatggagca
     1921 gtatataaag gctccttgga tgagcgtcca gttgctgtaa aagtgttttc ctttgcaaac
     1981 cgtcagaatt ttatcaacga aaagaacatt tacagagtgc ctttgatgga acatgacaac
     2041 attgcccgct ttatagttgg agatgagaga gtcactgcag atggacgcat ggaatatttg
     2101 cttgtgatgg agtactatcc caatggatct ttatgcaagt atttaagtct ccacacaagt
     2161 gactgggtaa gctcttgccg tcttgctcat tctgttacta gaggactggc ttatcttcac
     2221 acagaattac cacgaggaga tcattataaa cctgcaattt cccatcgaga tttaaacagc
     2281 agaaatgtcc tagtgaaaaa tgatggaacc tgtgttatta gtgactttgg actgtccatg
     2341 aggctgactg gaaatagact ggtgcgccca ggggaggaag ataatgcagc cataagcgag
     2401 gttggcacta tcagatatat ggcaccagaa gtgctagaag gagctgtgaa cttgagggac
     2461 tgtgaatcag ctttgaaaca agtagacatg tatgctcttg gactaatcta ttgggagata
     2521 tttatgagat gtacagacct cttcccaggg gaatccgtac cagagtacca gatggctttt
     2581 cagacagagg ttggaaacca tcccactttt gaggatatgc aggttctcgt gtctagggaa
     2641 aaacagagac ccaagttccc agaagcctgg aaagaaaata gcctggcagt gaggtcactc
     2701 aaggagacaa tcgaagactg ttgggaccag gatgcagagg ctcggcttac tgcacagtgt
     2761 gctgaggaaa ggatggctga acttatgatg atttgggaaa gaaacaaatc tgtgagccca
     2821 acagtcaatc caatgtctac tgctatgcag aatgaacgca acctgtcaca taataggcgt
     2881 gtgccaaaaa ttggtcctta tccagattat tcttcctcct catacattga agactctatc
     2941 catcatactg acagcatcgt gaagaatatt tcctctgagc attctatgtc cagcacacct
     3001 ttgactatag gggaaaaaaa ccgaaattca attaactatg aacgacagca agcacaagct
     3061 cgaatcccca gccctgaaac aagtgtcacc agcctctcca ccaacacaac aaccacaaac
     3121 accacaggac tcacgccaag tactggcatg actactatat ctgagatgcc atacccagat
     3181 gaaacaaatc tgcataccac aaatgttgca cagtcaattg ggccaacccc tgtctgctta
     3241 cagctgacag aagaagactt ggaaaccaac aagctagacc caaaagaagt tgataagaac
     3301 ctcaaggaaa gctctgatga gaatctcatg gagcactctc ttaaacagtt cagtggccca
     3361 gacccactga gcagtactag ttctagcttg ctttacccac tcataaaact tgcagtagaa
     3421 gcaactggac agcaggactt cacacagact gcaaatggcc aagcatgttt gattcctgat
     3481 gttctgccta ctcagatcta tcctctcccc aagcagcaga accttcccaa gagacctact
     3541 agtttgcctt tgaacaccaa aaattcaaca aaagagcccc ggctaaaatt tggcagcaag
     3601 cacaaatcaa acttgaaaca agtcgaaact ggagttgcca agatgaatac aatcaatgca
     3661 gcagaacctc atgtggtgac agtcaccatg aatggtgtgg caggtagaaa ccacagtgtt
     3721 aactcccatg ctgccacaac ccaatatgcc aatgggacag tactatctgg ccaaacaacc
     3781 aacatagtga cacatagggc ccaagaaatg ttgcagaatc agtttattgg tgaggacacc
     3841 cggctgaata ttaattccag tcctgatgag catgagcctt tactgagacg agagcaacaa
     3901 gctggccatg atgaaggtgt tctggatcgt cttgtggaca ggagggaacg gccactagaa
     3961 ggtggccgaa ctaattccaa taacaacaac agcaatccat gttcagaaca agatgttctt
     4021 gcacagggtg ttccaagcac agcagcagat cctgggccat caaagcccag aagagcacag
     4081 aggcctaatt ctctggatct ttcagccaca aatgtcctgg atggcagcag tatacagata
     4141 ggtgagtcaa cacaagatgg caaatcagga tcaggtgaaa agatcaagaa acgtgtgaaa
     4201 actccctatt ctcttaagcg gtggcgcccc tccacctggg tcatctccac tgaatcgctg
     4261 gactgtgaag tcaacaataa tggcagtaac agggcagttc attccaaatc cagcactgct
     4321 gtttaccttg cagaaggagg cactgctaca accatggtgt ctaaagatat aggaatgaac
     4381 tgtctgtaga acccagcttt cttgtacaaa gtggtgatat ccaattaacc ctcactaaag
     4441 ggtgtaaatg tttgagatgt atattcccta tagtgagtcg tattaccacc caacttttct
     4501 atacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     4561 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta
     4621 atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     4681 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     4741 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     4801 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     4861 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     4921 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     4981 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     5041 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     5101 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     5161 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg accgagtaca
     5221 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     5281 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     5341 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     5401 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     5461 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     5521 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     5581 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     5641 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     5701 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     5761 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctaaatc gatagatcct
     5821 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5881 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5941 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6001 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6061 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6121 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6181 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6241 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6301 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6361 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgagatcctt
     6421 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     6481 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     6541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     6721 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     6781 gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg aaagggcctc
     6841 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     6901 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     6961 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     7021 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     7081 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     7141 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     7201 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     7261 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     7321 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     7381 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     7441 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     7501 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     7561 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     7621 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     7681 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     7741 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     7801 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     7861 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     7921 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     7981 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8041 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8101 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8161 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     8221 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8281 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8341 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8401 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8461 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8521 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8581 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8641 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8701 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8761 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8821 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     8881 agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8941 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9001 tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9061 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9121 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
     9181 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9241 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     9301 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     9361 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     9421 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     9481 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     9541 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     9601 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     9661 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     9721 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     9781 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     9841 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     9901 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     9961 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
    10021 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
    10081 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
    10141 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
    10201 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
    10261 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
    10321 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
    10381 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
    10441 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
    10501 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
    10561 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    10621 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    10681 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    10741 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    10801 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    10861 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    10921 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    10981 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    11041 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    11101 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    11161 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    11221 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    11281 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    11341 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    11401 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga
    11461 attcacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    11521 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    11581 aacaaattac aaaaattcaa aatttt
//
