LOCUS       sequence-250523-       12128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR Y764_V765insHH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-Y764_V765insHH
FEATURES             Location/Qualifiers
     source          1..12128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3448..3519,3520..5382,5383..5451,5452..7086)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYHHVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIG
                     SQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEY
                     HAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILE
                     KGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERM
                     HLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNST
                     VACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSV
                     QNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQ
                     ISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(7088..7112)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7120..7138
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7163..7182)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7164..7182)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7188..7208)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7262..7303
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7324..7891
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7341..7893
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7485..7502)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7712..7731
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7905..8504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7905..7924)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8401..8421
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8518..9106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8571..8591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8989..9000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9181..9414
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9533..9551)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9619..9723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9724..9792,9793..10584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10755..11343
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11244..11263
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11497..11514
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11754..11775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11790..11795,11796..11813,11814..11820)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11828..11844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11833..11855
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11852..11868
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11852..11868
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11918..11937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg cgaccctccg ggacggccgg ggcagcgctc
     3481 ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc
     3541 caaggcacga gtaacaagct cacgcagttg ggcacttttg aagatcattt tctcagcctc
     3601 cagaggatgt tcaataactg tgaggtggtc cttgggaatt tggaaattac ctatgtgcag
     3661 aggaattatg atctttcctt cttaaagacc atccaggagg tggctggtta tgtcctcatt
     3721 gccctcaaca cagtggagcg aattcctttg gaaaacctgc agatcatcag aggaaatatg
     3781 tactacgaaa attcctatgc cttagcagtc ttatctaact atgatgcaaa taaaaccgga
     3841 ctgaaggagc tgcccatgag aaatttacag gaaatcctgc atggcgccgt gcggttcagc
     3901 aacaaccctg ccctgtgcaa cgtggagagc atccagtggc gggacatagt cagcagtgac
     3961 tttctcagca acatgtcgat ggacttccag aaccacctgg gcagctgcca aaagtgtgat
     4021 ccaagctgtc ccaatgggag ctgctggggt gcaggagagg agaactgcca gaaactgacc
     4081 aaaatcatct gtgcccagca gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc
     4141 tgccacaacc agtgtgctgc aggctgcaca ggcccccggg agagcgactg cctggtctgc
     4201 cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc ccccactcat gctctacaac
     4261 cccaccacgt accagatgga tgtgaacccc gagggcaaat acagctttgg tgccacctgc
     4321 gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt
     4381 ggggccgaca gctatgagat ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg
     4441 ccttgccgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc actctccata
     4501 aatgctacga atattaaaca cttcaaaaac tgcacctcca tcagtggcga tctccacatc
     4561 ctgccggtgg catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa
     4621 ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct
     4681 gaaaacagga cggacctcca tgcctttgag aacctagaaa tcatacgcgg caggaccaag
     4741 caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt gggattacgc
     4801 tccctcaagg agataagtga tggagatgtg ataatttcag gaaacaaaaa tttgtgctat
     4861 gcaaatacaa taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata
     4921 agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc
     4981 cccgagggct gctggggccc ggagcccagg gactgcgtct cttgccggaa tgtcagccga
     5041 ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga gtttgtggag
     5101 aactctgagt gcatacagtg ccacccagag tgcctgcctc aggccatgaa catcacctgc
     5161 acaggacggg gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc
     5221 gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca
     5281 gacgccggcc atgtgtgcca cctgtgccat ccaaactgca cctacggatg cactgggcca
     5341 ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac tgggatggtg
     5401 ggggccctcc tcttgctgct ggtggtggcc ctggggatcg gcctcttcat gcgaaggcgc
     5461 cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg agagggagct tgtggagcct
     5521 cttacaccca gtggagaagc tcccaaccaa gctctcttga ggatcttgaa ggaaactgaa
     5581 ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca cggtgtataa gggactctgg
     5641 atcccagaag gtgagaaagt taaaattccc gtcgctatca aggaattaag agaagcaaca
     5701 tctccgaaag ccaacaagga aatcctcgat gaagcctacc atcatgtgat ggccagcgtg
     5761 gacaaccccc acgtgtgccg cctgctgggc atctgcctca cctccaccgt gcagctcatc
     5821 acgcagctca tgcccttcgg ctgcctcctg gactatgtcc gggaacacaa agacaatatt
     5881 ggctcccagt acctgctcaa ctggtgtgtg cagatcgcaa agggcatgaa ctacttggag
     5941 gaccgtcgct tggtgcaccg cgacctggca gccaggaacg tactggtgaa aacaccgcag
     6001 catgtcaaga tcacagattt tgggctggcc aaactgctgg gtgcggaaga gaaagaatac
     6061 catgcagaag gaggcaaagt gcctatcaag tggatggcat tggaatcaat tttacacaga
     6121 atctataccc accagagtga tgtctggagc tacggggtga ccgtttggga gttgatgacc
     6181 tttggatcca agccatatga cggaatccct gccagcgaga tctcctccat cctggagaaa
     6241 ggagaacgcc tccctcagcc acccatatgt accatcgatg tctacatgat catggtcaag
     6301 tgctggatga tagacgcaga tagtcgccca aagttccgtg agttgatcat cgaattctcc
     6361 aaaatggccc gagaccccca gcgctacctt gtcattcagg gggatgaaag aatgcatttg
     6421 ccaagtccta cagactccaa cttctaccgt gccctgatgg atgaagaaga catggacgac
     6481 gtggtggatg ccgacgagta cctcatccca cagcagggct tcttcagcag cccctccacg
     6541 tcacggactc ccctcctgag ctctctgagt gcaaccagca acaattccac cgtggcttgc
     6601 attgatagaa atgggctgca aagctgtccc atcaaggaag acagcttctt gcagcgatac
     6661 agctcagacc ccacaggcgc cttgactgag gacagcatag acgacacctt cctcccagtg
     6721 cctgaataca taaaccagtc cgttcccaaa aggcccgctg gctctgtgca gaatcctgtc
     6781 tatcacaatc agcctctgaa ccccgcgccc agcagagacc cacactacca ggacccccac
     6841 agcactgcag tgggcaaccc cgagtatctc aacactgtcc agcccacctg tgtcaacagc
     6901 acattcgaca gccctgccca ctgggcccag aaaggcagcc accaaattag cctggacaac
     6961 cctgactacc agcaggactt ctttcccaag gaagccaagc caaatggcat ctttaagggc
     7021 tccacagctg aaaatgcaga atacctaagg gtcgcgccac aaagcagtga atttattgga
     7081 gcatagaacc cagctttctt gtacaaagtg gtgatatcca attaaccctc actaaaggtt
     7141 tgttagaatg agaagattta tgccctatag tgagtcgtat taccacccaa cttttctata
     7201 caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga
     7261 aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg
     7321 agatccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     7381 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     7441 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     7501 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     7561 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     7621 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     7681 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     7741 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     7801 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     7861 cgtggttttc ctttgaaaaa cacgatgata atatggccac acatatgacc gagtacaagc
     7921 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     7981 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     8041 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     8101 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     8161 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     8221 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     8281 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     8341 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     8401 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     8461 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctaaatcgat agatcctaat
     8521 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8581 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8641 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8701 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8761 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8821 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8881 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     8941 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     9001 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     9061 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa
     9121 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     9181 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     9241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     9301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     9361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     9421 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9481 agaggcccgg gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg
     9541 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     9601 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9661 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9721 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9841 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9901 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9961 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    10021 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    10081 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    10141 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    10201 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    10261 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10321 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    10381 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    10441 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10501 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10561 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10621 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10681 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10741 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10801 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10861 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    10921 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10981 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    11041 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    11101 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    11161 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    11221 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    11281 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11341 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    11401 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    11461 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    11521 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    11581 aagctcatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    11641 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
    11701 ccgtggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
    11761 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
    11821 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
    11881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    11941 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    12001 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    12061 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    12121 ttagtagt
//
