LOCUS       sequence-250533-       11443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EPHA2 G391R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EPHA2-G391R
FEATURES             Location/Qualifiers
     source          1..11443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6380..6404)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6457..6498
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6519..7086
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6536..7088
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6680..6697)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6907..6926
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7100..7102,7103..7105,7106..7819)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7100..7102,7103..7105,7106..7816)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7145..7166)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7406..7425)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7753..7774
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7833..8421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7886..7906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8304..8315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8496..8729
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8848..8866)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8934..9038
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9039..9107,9108..9899)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9257..9276)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10070..10658
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10559..10578
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10812..10829
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11069..11090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11105..11110,11111..11128,11129..11135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11143..11159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11148..11170
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11167..11183
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11167..11183
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11233..11252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gagctccagg cagcccgcgc ctgcttcgcc
     3481 ctgctgtggg gctgtgcgct ggccgcggcc gcggcggcgc agggcaagga agtggtactg
     3541 ctggactttg ctgcagctgg aggggagctc ggctggctca cacacccgta tggcaaaggg
     3601 tgggacctga tgcagaacat catgaatgac atgccgatct acatgtactc cgtgtgcaac
     3661 gtgatgtctg gcgaccagga caactggctc cgcaccaact gggtgtaccg aggagaggct
     3721 gagcgtatct tcattgagct caagtttact gtacgtgact gcaacagctt ccctggtggc
     3781 gccagctcct gcaaggagac tttcaacctc tactatgccg agtcggacct ggactacggc
     3841 accaacttcc agaagcgcct gttcaccaag attgacacca ttgcgcccga tgagatcacc
     3901 gtcagcagcg acttcgaggc acgccacgtg aagctgaacg tggaggagcg ctccgtgggg
     3961 ccgctcaccc gcaaaggctt ctacctggcc ttccaggata tcggtgcctg tgtggcgctg
     4021 ctctccgtcc gtgtctacta caagaagtgc cccgagctgc tgcagggcct ggcccacttc
     4081 cctgagacca tcgccggctc tgatgcacct tccctggcca ctgtggccgg cacctgtgtg
     4141 gaccatgccg tggtgccacc ggggggtgaa gagccccgta tgcactgtgc agtggatggc
     4201 gagtggctgg tgcccattgg gcagtgcctg tgccaggcag gctacgagaa ggtggaggat
     4261 gcctgccagg cctgctcgcc tggatttttt aagtttgagg catctgagag cccctgcttg
     4321 gagtgccctg agcacacgct gccatcccct gagggtgcca cctcctgcga gtgtgaggaa
     4381 ggcttcttcc gggcacctca ggacccagcg tcgatgcctt gcacacgacc cccctccgcc
     4441 ccacactacc tcacagccgt gggcatgggt gccaaggtgg agctgcgctg gacgccccct
     4501 caggacagcg ggggccgcga ggacattgtc tacagcgtca cctgcgaaca gtgctggccc
     4561 gagtctgggg aatgcgggcc gtgtgaggcc agtgtgcgct actcggagcc tcctcacaga
     4621 ctgacccgca ccagtgtgac agtgagcgac ctggagcccc acatgaacta caccttcacc
     4681 gtggaggccc gcaatggcgt ctcaggcctg gtaaccagcc gcagcttccg tactgccagt
     4741 gtcagcatca accagacaga gccccccaag gtgaggctgg agggccgcag caccacctcg
     4801 cttagcgtct cctggagcat ccccccgccg cagcagagcc gagtgtggaa gtacgaggtc
     4861 acttaccgca agaagggaga ctccaacagc tacaatgtgc gccgcaccga gggtttctcc
     4921 gtgaccctgg acgacctggc cccagacacc acctacctgg tccaggtgca ggcactgacg
     4981 caggagggcc agggggccgg cagcaaggtg cacgaattcc agacgctgtc cccggaggga
     5041 tctggcaact tggcggtgat tggcggcgtg gctgtcggtg tggtcctgct tctggtgctg
     5101 gcaggagttg gcttctttat ccaccgcagg aggaagaacc agcgtgcccg ccagtccccg
     5161 gaggacgttt acttctccaa gtcagaacaa ctgaagcccc tgaagacata cgtggacccc
     5221 cacacatatg aggaccccaa ccaggctgtg ttgaagttca ctaccgagat ccatccatcc
     5281 tgtgtcactc ggcagaaggt gatcggagca ggagagtttg gggaggtgta caagggcatg
     5341 ctgaagacat cctcggggaa gaaggaggtg ccggtggcca tcaagacgct gaaagccggc
     5401 tacacagaga agcagcgagt ggacttcctc ggcgaggccg gcatcatggg ccagttcagc
     5461 caccacaaca tcatccgcct agagggcgtc atctccaaat acaagcccat gatgatcatc
     5521 actgagtaca tggagaatgg ggccctggac aagttccttc gggagaagga tggcgagttc
     5581 agcgtgctgc agctggtggg catgctgcgg ggcatcgcag ctggcatgaa gtacctggcc
     5641 aacatgaact atgtgcaccg tgacctggct gcccgcaaca tcctcgtcaa cagcaacctg
     5701 gtctgcaagg tgtctgactt tggcctgtcc cgcgtgctgg aggacgaccc cgaggccacc
     5761 tacaccacca gtggcggcaa gatccccatc cgctggaccg ccccggaggc catttcctac
     5821 cggaagttca cctctgccag cgacgtgtgg agctttggca ttgtcatgtg ggaggtgatg
     5881 acctatggcg agcggcccta ctgggagttg tccaaccacg aggtgatgaa agccatcaat
     5941 gatggcttcc ggctccccac acccatggac tgcccctccg ccatctacca gctcatgatg
     6001 cagtgctggc agcaggagcg tgcccgccgc cccaagttcg ctgacatcgt cagcatcctg
     6061 gacaagctca ttcgtgcccc tgactccctc aagaccctgg ctgactttga cccccgcgtg
     6121 tctatccggc tccccagcac gagcggctcg gagggggtgc ccttccgcac ggtgtccgag
     6181 tggctggagt ccatcaagat gcagcagtat acggagcact tcatggcggc cggctacact
     6241 gccatcgaga aggtggtgca gatgaccaac gacgacatca agaggattgg ggtgcggctg
     6301 cccggccacc agaagcgcat cgcctacagc ctgctgggac tcaaggacca ggtgaacact
     6361 gtggggatcc ccatctagaa cccagctttc ttgtacaaag tggttgatat ccagcacagt
     6421 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
     6481 ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc ccctaacgtt
     6541 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     6601 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     6661 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     6721 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     6781 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     6841 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     6901 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     6961 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     7021 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     7081 tgataatatg gccacacata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     7141 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     7201 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     7261 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     7321 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     7381 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     7441 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     7501 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     7561 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     7621 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     7681 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     7741 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     7801 ggacgagctg tacaagtaaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg
     7861 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7921 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7981 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8041 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8101 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8161 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8221 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8281 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8341 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8401 ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt
     8461 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     8521 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8581 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8641 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8701 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     8761 tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag
     8821 ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8881 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8941 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     9001 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     9061 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     9121 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     9181 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     9241 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     9301 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     9361 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     9421 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     9481 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     9541 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     9601 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     9661 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     9721 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     9781 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9841 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9901 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9961 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    10021 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    10081 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    10141 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    10201 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    10261 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    10321 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    10381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    10441 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    10501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    10561 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    10621 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    10681 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    10741 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    10801 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
    10861 ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac taattttttt
    10921 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
    10981 cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact
    11041 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
    11101 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
    11161 ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac
    11221 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    11281 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    11341 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11401 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
