LOCUS       sequence-250534-       10816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF A598_T599insP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10816)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-A598_T599insP
FEATURES             Location/Qualifiers
     source          1..10816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2352..2376)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2429..2470
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2491..3058
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2508..3060
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2652..2669)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2879..2898
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3072..3074,3075..3077,3078..3791)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3072..3074,3075..3077,3078..3788)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3117..3138)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3378..3397)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3725..3746
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3805..4393
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3858..3878)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4276..4287)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4468..4701
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4820..4838)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4906..5010
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5011..5079,5080..5871)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5229..5248)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6042..6630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6531..6550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6784..6801
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7041..7062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7077..7082,7083..7100,7101..7107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7115..7131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7120..7142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7139..7155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7139..7155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7205..7224
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7416..8049
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8096..8221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8718..8951
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9136..9180
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9473..9590
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9638..10816
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9869..10807
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10764..10784
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg cggcgctgag
       61 cggtggcggt ggtggcggcg cggagccggg ccaggctctg ttcaacgggg acatggagcc
      121 cgaggccggc gccggcgccg gcgccgcggc ctcttcggct gcggaccctg ccattccgga
      181 ggaggtgtgg aatatcaaac aaatgattaa gttgacacag gaacatatag aggccctatt
      241 ggacaaattt ggtggggagc ataatccacc atcaatatat ctggaggcct atgaagaata
      301 caccagcaag ctagatgcac tccaacaaag agaacaacag ttattggaat ctctggggaa
      361 cggaactgat ttttctgttt ctagctctgc atcaatggat accgttacat cttcttcctc
      421 ttctagcctt tcagtgctac cttcatctct ttcagttttt caaaatccca cagatgtggc
      481 acggagcaac cccaagtcac cacaaaaacc tatcgttaga gtcttcctgc ccaacaaaca
      541 gaggacagtg gtacctgcaa ggtgtggagt tacagtccga gacagtctaa agaaagcact
      601 gatgatgaga ggtctaatcc cagagtgctg tgctgtttac agaattcagg atggagagaa
      661 gaaaccaatt ggttgggaca ctgatatttc ctggcttact ggagaagaat tgcatgtgga
      721 agtgttggag aatgttccac ttacaacaca caactttgta cgaaaaacgt ttttcacctt
      781 agcattttgt gacttttgtc gaaagctgct tttccagggt ttccgctgtc aaacatgtgg
      841 ttataaattt caccagcgtt gtagtacaga agttccactg atgtgtgtta attatgacca
      901 acttgatttg ctgtttgtct ccaagttctt tgaacaccac ccaataccac aggaagaggc
      961 gtccttagca gagactgccc taacatctgg atcatcccct tccgcacccg cctcggactc
     1021 tattgggccc caaattctca ccagtccgtc tccttcaaaa tccattccaa ttccacagcc
     1081 cttccgacca gcagatgaag atcatcgaaa tcaatttggg caacgagacc gatcctcatc
     1141 agctcccaat gtgcatataa acacaataga acctgtcaat attgatgact tgattagaga
     1201 ccaaggattt cgtggtgatg gaggatcaac cacaggtttg tctgctaccc cccctgcctc
     1261 attacctggc tcactaacta acgtgaaagc cttacagaaa tctccaggac ctcagcgaga
     1321 aaggaagtca tcttcatcct cagaagacag gaatcgaatg aaaacacttg gtagacggga
     1381 ctcgagtgat gattgggaga ttcctgatgg gcagattaca gtgggacaaa gaattggatc
     1441 tggatcattt ggaacagtct acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt
     1501 gaatgtgaca gcacctacac ctcagcagtt acaagccttc aaaaatgaag taggagtact
     1561 caggaaaaca cgacatgtga atatcctact cttcatgggc tattccacaa agccacaact
     1621 ggctattgtt acccagtggt gtgagggctc cagcttgtat caccatctcc atatcattga
     1681 gaccaaattt gagatgatca aacttataga tattgcacga cagactgcac agggcatgga
     1741 ttacttacac gccaagtcaa tcatccacag agacctcaag agtaataata tatttcttca
     1801 tgaagacctc acagtaaaaa taggtgattt tggtctagct ccaacagtga aatctcgatg
     1861 gagtgggtcc catcagtttg aacagttgtc tggatccatt ttgtggatgg caccagaagt
     1921 catcagaatg caagataaaa atccatacag ctttcagtca gatgtatatg catttggaat
     1981 tgttctgtat gaattgatga ctggacagtt accttattca aacatcaaca acagggacca
     2041 gataattttt atggtgggac gaggatacct gtctccagat ctcagtaagg tacggagtaa
     2101 ctgtccaaaa gccatgaaga gattaatggc agagtgcctc aaaaagaaaa gagatgagag
     2161 accactcttt ccccaaattc tcgcctctat tgagctgctg gcccgctcat tgccaaaaat
     2221 tcaccgcagt gcatcagaac cctccttgaa tcgggctggt ttccaaacag aggattttag
     2281 tctatatgct tgtgcttctc caaaaacacc catccaggca gggggatatg gtgcgtttcc
     2341 tgtccactag aacccagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg
     2401 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     2461 cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg
     2521 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     2581 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     2641 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2701 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     2761 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     2821 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     2881 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     2941 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     3001 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     3061 tggccacaca tatggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3121 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3181 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3241 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3301 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3361 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3421 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3481 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3541 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     3601 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3661 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     3721 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     3781 tgtacaagta aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     3841 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3901 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3961 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     4021 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     4081 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     4141 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     4201 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     4261 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     4321 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     4381 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
     4441 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     4501 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     4561 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     4621 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     4681 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     4741 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
     4801 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     4861 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4921 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4981 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     5041 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     5101 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     5161 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     5221 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     5281 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     5341 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     5401 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     5461 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     5521 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     5581 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     5641 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     5701 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5761 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5821 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5881 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5941 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     6001 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     6061 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     6121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     6181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     6241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     6301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     6361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     6421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     6481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     6541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     6601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     6661 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6721 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6781 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6841 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
     6901 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6961 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
     7021 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7081 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7141 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
     7201 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     7261 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     7321 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     7381 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     7441 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     7501 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     7561 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     7621 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     7681 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     7741 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     7801 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     7861 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7921 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7981 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8041 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     8101 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8161 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     8221 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     8281 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     8341 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     8401 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     8461 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     8521 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     8581 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     8641 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     8701 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     8761 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     8821 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     8881 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     8941 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     9001 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     9061 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     9121 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     9181 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     9241 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     9301 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     9361 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     9421 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa
     9481 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     9541 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     9601 acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc
     9661 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     9721 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     9781 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     9841 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     9901 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     9961 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
    10021 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
    10081 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
    10141 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
    10201 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
    10261 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
    10321 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
    10381 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
    10441 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
    10501 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
    10561 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
    10621 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
    10681 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
    10741 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
    10801 atttcaggtg tcgtga
//
