LOCUS       sequence-250537-       11464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EPHA3 R750L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11464)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EPHA3-R750L
FEATURES             Location/Qualifiers
     source          1..11464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6401..6425)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6478..6519
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6540..7107
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6557..7109
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6701..6718)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6928..6947
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7121..7123,7124..7126,7127..7840)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7121..7123,7124..7126,7127..7837)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7166..7187)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7427..7446)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7774..7795
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7854..8442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7907..7927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8325..8336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8517..8750
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8869..8887)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8955..9059
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9060..9128,9129..9920)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9278..9297)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10091..10679
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10580..10599
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10833..10850
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11090..11111
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11126..11131,11132..11149,11150..11156)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11164..11180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11169..11191
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11188..11204
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11188..11204
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11254..11273
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gattgtcagc tctccatcct cctccttctc
     3481 agctgctctg ttctcgacag cttcggggaa ctgattccgc agccttccaa tgaagtcaat
     3541 ctactggatt caaaaacaat tcaaggggag ctgggctgga tctcttatcc atcacatggg
     3601 tgggaagaga tcagtggtgt ggatgaacat tacacaccca tcaggactta ccaggtgtgc
     3661 aatgtcatgg accacagtca aaacaattgg ctgagaacaa actgggtccc caggaactca
     3721 gctcagaaga tttatgtgga gctcaagttc actctacgag actgcaatag cattccattg
     3781 gttttaggaa cttgcaagga gacattcaac ctgtactaca tggagtctga tgatgatcat
     3841 ggggtgaaat ttcgagagca tcagtttaca aagattgaca ccattgcagc tgatgaaagt
     3901 ttcactcaaa tggatcttgg ggaccgtatt ctgaagctca acactgagat tagagaagta
     3961 ggtcctgtca acaagaaggg attttatttg gcatttcaag atgttggtgc ttgtgttgcc
     4021 ttggtgtctg tgagagtata cttcaaaaag tgcccattta cagtgaagaa tctggctatg
     4081 tttccagaca cggtacccat ggactcccag tccctggtgg aggttagagg gtcttgtgtc
     4141 aacaattcta aggaggaaga tcctccaagg atgtactgca gtacagaagg cgaatggctt
     4201 gtacccattg gcaagtgttc ctgcaatgct ggctatgaag aaagaggttt tatgtgccaa
     4261 gcttgtcgac caggtttcta caaggcattg gatggtaata tgaagtgtgc taagtgcccg
     4321 cctcacagtt ctactcagga agatggttca atgaactgca ggtgtgagaa taattacttc
     4381 cgggcagaca aagaccctcc atccatggct tgtacccgac ctccatcttc accaagaaat
     4441 gttatctcta atataaacga gacctcagtt atcctggact ggagttggcc cctggacaca
     4501 ggaggccgga aagatgttac cttcaacatc atatgtaaaa aatgtgggtg gaatataaaa
     4561 cagtgtgagc catgcagccc aaatgtccgc ttcctccctc gacagtttgg actcaccaac
     4621 accacggtga cagtgacaga ccttctggca catactaact acacctttga gattgatgcc
     4681 gttaatgggg tgtcagagct gagctcccca ccaagacagt ttgctgcggt cagcatcaca
     4741 actaatcagg ctgctccatc acctgtcctg acgattaaga aagatcggac ctccagaaat
     4801 agcatctctt tgtcctggca agaacctgaa catcctaatg ggatcatatt ggactacgag
     4861 gtcaaatact atgaaaagca ggaacaagaa acaagttata ccattctgag ggcaagaggc
     4921 acaaatgtta ccatcagtag cctcaagcct gacactatat acgtattcca aatccgagcc
     4981 cgaacagccg ctggatatgg gacgaacagc cgcaagtttg agtttgaaac tagtccagac
     5041 tctttctcca tctctggtga aagtagccaa gtggtcatga tcgccatttc agcggcagta
     5101 gcaattattc tcctcactgt tgtcatctat gttttgattg ggaggttctg tggctataag
     5161 tcaaaacatg gggcagatga aaaaagactt cattttggca atgggcattt aaaacttcca
     5221 ggtctcagga cttatgttga cccacataca tatgaagacc ctacccaagc tgttcatgag
     5281 tttgccaagg aattggatgc caccaacata tccattgata aagttgttgg agcaggtgaa
     5341 tttggagagg tgtgcagtgg tcgcttaaaa cttccttcaa aaaaagagat ttcagtggcc
     5401 attaagaccc tgaaagttgg ctacacagaa aagcagagga gagacttcct gggagaagca
     5461 agcattatgg gacagtttga ccaccccaat atcattcgac tggaaggagt tgttaccaaa
     5521 agtaagccag ttatgattgt cacagaatac atggagaatg gttccttgga tagtttccta
     5581 cgtaaacacg atgcccagtt tactgtcatt cagctagtgg ggatgcttcg agggatagca
     5641 tctggcatga agtacctgtc agacatgggc tatgttcacc gagacctcgc tgctctgaac
     5701 atcttgatca acagtaactt ggtgtgtaag gtttctgatt tcggactttc gcgtgtcctg
     5761 gaggatgacc cagaagctgc ttatacaaca agaggaggga agatcccaat caggtggaca
     5821 tcaccagaag ctatagccta ccgcaagttc acgtcagcca gcgatgtatg gagttatggg
     5881 attgttctct gggaggtgat gtcttatgga gagagaccat actgggagat gtccaatcag
     5941 gatgtaatta aagctgtaga tgagggctat cgactgccac cccccatgga ctgcccagct
     6001 gccttgtatc agctgatgct ggactgctgg cagaaagaca ggaacaacag acccaagttt
     6061 gagcagattg ttagtattct ggacaagctt atccggaatc ccggcagcct gaagatcatc
     6121 accagtgcag ccgcaaggcc atcaaacctt cttctggacc aaagcaatgt ggatatcact
     6181 accttccgca caacaggtga ctggcttaat ggtgtctgga cagcacactg caaggaaatc
     6241 ttcacgggtg tggagtacag ttcttgtgac acaatagcca agatttccac agatgacatg
     6301 aaaaaggttg gtgtcaccgt ggttgggcca cagaagaaga tcatcagtag cattaaagct
     6361 ctagaaacgc aatcaaagaa tggcccagtt cccgtgtaga acccagcttt cttgtacaaa
     6421 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     6481 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagat
     6541 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     6601 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     6661 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6721 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     6781 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     6841 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     6901 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     6961 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     7021 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     7081 gttttccttt gaaaaacacg atgataatat ggccacacat atggtgagca agggcgagga
     7141 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     7201 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     7261 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     7321 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     7381 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     7441 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     7501 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     7561 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     7621 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     7681 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     7741 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     7801 cgccgggatc actctcggca tggacgagct gtacaagtaa atcgatagat cctaatcaac
     7861 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7921 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7981 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     8041 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     8101 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     8161 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     8221 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     8281 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     8341 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     8401 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     8461 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     8521 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     8581 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8641 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8701 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8761 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8821 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     8881 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8941 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9001 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9061 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9121 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9181 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9241 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9301 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9361 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9421 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9481 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9541 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9601 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9661 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9721 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9781 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9841 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9901 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9961 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10021 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10081 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10141 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10201 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10261 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10321 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10381 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10441 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10501 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10561 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10621 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10681 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10741 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10801 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10861 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
    10921 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10981 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
    11041 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    11101 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    11161 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
    11221 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    11281 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    11341 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    11401 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    11461 tagt
//
