LOCUS       sequence-250539-       10791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF T599Ifs*13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-T599Ifs*13
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2350..2374)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2382..2400
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2425..2444)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2426..2444)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2450..2470)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             2524..2565
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2586..3153
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2603..3155
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2747..2764)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2974..2993
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3167..3766
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3167..3186)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3663..3683
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3780..4368
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3833..3853)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4251..4262)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4443..4676
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4795..4813)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4881..4985
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4986..5054,5055..5846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5204..5223)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6017..6605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6506..6525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6759..6776
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7016..7037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7052..7057,7058..7075,7076..7082)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7090..7106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7095..7117
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7114..7130
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7114..7130
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7180..7199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7391..8024
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8071..8196
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8693..8926
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9111..9155
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9448..9565
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9613..10791
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9844..10782
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10739..10759
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg cggcgctgag
       61 cggtggcggt ggtggcggcg cggagccggg ccaggctctg ttcaacgggg acatggagcc
      121 cgaggccggc gccggcgccg gcgccgcggc ctcttcggct gcggaccctg ccattccgga
      181 ggaggtgtgg aatatcaaac aaatgattaa gttgacacag gaacatatag aggccctatt
      241 ggacaaattt ggtggggagc ataatccacc atcaatatat ctggaggcct atgaagaata
      301 caccagcaag ctagatgcac tccaacaaag agaacaacag ttattggaat ctctggggaa
      361 cggaactgat ttttctgttt ctagctctgc atcaatggat accgttacat cttcttcctc
      421 ttctagcctt tcagtgctac cttcatctct ttcagttttt caaaatccca cagatgtggc
      481 acggagcaac cccaagtcac cacaaaaacc tatcgttaga gtcttcctgc ccaacaaaca
      541 gaggacagtg gtacctgcaa ggtgtggagt tacagtccga gacagtctaa agaaagcact
      601 gatgatgaga ggtctaatcc cagagtgctg tgctgtttac agaattcagg atggagagaa
      661 gaaaccaatt ggttgggaca ctgatatttc ctggcttact ggagaagaat tgcatgtgga
      721 agtgttggag aatgttccac ttacaacaca caactttgta cgaaaaacgt ttttcacctt
      781 agcattttgt gacttttgtc gaaagctgct tttccagggt ttccgctgtc aaacatgtgg
      841 ttataaattt caccagcgtt gtagtacaga agttccactg atgtgtgtta attatgacca
      901 acttgatttg ctgtttgtct ccaagttctt tgaacaccac ccaataccac aggaagaggc
      961 gtccttagca gagactgccc taacatctgg atcatcccct tccgcacccg cctcggactc
     1021 tattgggccc caaattctca ccagtccgtc tccttcaaaa tccattccaa ttccacagcc
     1081 cttccgacca gcagatgaag atcatcgaaa tcaatttggg caacgagacc gatcctcatc
     1141 agctcccaat gtgcatataa acacaataga acctgtcaat attgatgact tgattagaga
     1201 ccaaggattt cgtggtgatg gaggatcaac cacaggtttg tctgctaccc cccctgcctc
     1261 attacctggc tcactaacta acgtgaaagc cttacagaaa tctccaggac ctcagcgaga
     1321 aaggaagtca tcttcatcct cagaagacag gaatcgaatg aaaacacttg gtagacggga
     1381 ctcgagtgat gattgggaga ttcctgatgg gcagattaca gtgggacaaa gaattggatc
     1441 tggatcattt ggaacagtct acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt
     1501 gaatgtgaca gcacctacac ctcagcagtt acaagccttc aaaaatgaag taggagtact
     1561 caggaaaaca cgacatgtga atatcctact cttcatgggc tattccacaa agccacaact
     1621 ggctattgtt acccagtggt gtgagggctc cagcttgtat caccatctcc atatcattga
     1681 gaccaaattt gagatgatca aacttataga tattgcacga cagactgcac agggcatgga
     1741 ttacttacac gccaagtcaa tcatccacag agacctcaag agtaataata tatttcttca
     1801 tgaagacctc acagtaaaaa taggtgattt tggtctagct atacgtgaaa tctcgatgga
     1861 gtgggtccca tcagtttgaa cagttgtctg gatccatttt gtggatggca ccagaagtca
     1921 tcagaatgca agataaaaat ccatacagct ttcagtcaga tgtatatgca tttggaattg
     1981 ttctgtatga attgatgact ggacagttac cttattcaaa catcaacaac agggaccaga
     2041 taatttttat ggtgggacga ggatacctgt ctccagatct cagtaaggta cggagtaact
     2101 gtccaaaagc catgaagaga ttaatggcag agtgcctcaa aaagaaaaga gatgagagac
     2161 cactctttcc ccaaattctc gcctctattg agctgctggc ccgctcattg ccaaaaattc
     2221 accgcagtgc atcagaaccc tccttgaatc gggctggttt ccaaacagag gattttagtc
     2281 tatatgcttg tgcttctcca aaaacaccca tccaggcagg gggatatggt gcgtttcctg
     2341 tccactagaa cccagctttc ttgtacaaag tggtgatatc caattaaccc tcactaaagg
     2401 ttgtgatagt agttagatat ttgtccctat agtgagtcgt attaccaccc aacttttcta
     2461 tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg cccgcggttc
     2521 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
     2581 tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     2641 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     2701 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     2761 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     2821 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     2881 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     2941 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     3001 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     3061 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     3121 gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatga ccgagtacaa
     3181 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     3241 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     3301 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     3361 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     3421 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     3481 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     3541 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     3601 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     3661 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     3721 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctaaatcg atagatccta
     3781 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3841 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3901 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3961 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     4021 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     4081 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     4141 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     4201 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     4261 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     4321 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
     4381 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4441 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4501 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4561 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4621 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     4681 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     4741 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
     4801 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4861 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4921 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4981 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5041 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5101 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5161 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5221 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5281 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5341 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5401 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5461 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5521 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5581 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5641 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5701 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5761 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5821 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5881 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5941 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6001 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6061 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6601 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6661 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6721 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6781 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6841 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6901 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6961 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     7021 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7081 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
     7141 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7201 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     7261 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     7321 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     7381 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     7441 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     7501 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     7561 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     7621 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     7681 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     7741 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     7801 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     7861 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7921 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7981 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     8041 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     8101 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     8161 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     8221 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     8281 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     8341 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     8401 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     8461 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8521 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     8581 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     8641 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     8701 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     8761 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     8821 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     8881 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     8941 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     9001 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     9061 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     9121 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     9181 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     9241 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     9301 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     9361 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa
     9421 ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     9481 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     9541 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     9601 gactagtcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     9661 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     9721 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     9781 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     9841 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     9901 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     9961 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
    10021 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
    10081 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
    10141 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
    10201 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
    10261 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
    10321 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
    10381 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
    10441 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
    10501 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
    10561 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
    10621 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
    10681 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
    10741 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg a
//
