LOCUS       sequence-250541-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR G719A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-G719A
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    296..413
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        461..1639
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          692..1630
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1587..1607
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1659..1683
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1686..1757,1758..3620,3621..3689,3690..5318)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLASGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5320..5344)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5352..5370
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5395..5414)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5396..5414)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5420..5440)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5494..5535
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5556..6123
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5573..6125
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5717..5734)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5944..5963
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6137..6736
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6137..6156)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6633..6653
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6750..7338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6803..6823)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7221..7232)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7413..7646
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7765..7783)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7851..7955
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7956..8024,8025..8816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8987..9575
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9476..9495
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9729..9746
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9986..10007
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10022..10027,10028..10045,10046..10052)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10060..10076
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10065..10087
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10084..10100
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10084..10100
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10150..10169
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10361..10994
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11041..11166
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11663..11896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(12081..12122,1..3)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
       61 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
      121 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
      181 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
      241 gtgaacggat ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta
      301 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
      361 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
      421 attacaggga cagcagagat ccagtttgga ctagtcgtga ggctccggtg cccgtcagtg
      481 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      541 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      601 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      661 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      721 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      781 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      841 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      901 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
      961 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     1021 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     1081 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1141 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1201 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1261 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1321 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1381 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1441 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1501 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1561 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1621 tccatttcag gtgtcgtgaa gcggccctgc agatatcaac aagtttgtac aaaaaagcag
     1681 gcaccatgcg accctccggg acggccgggg cagcgctcct ggcgctgctg gctgcgctct
     1741 gcccggcgag tcgggctctg gaggaaaaga aagtttgcca aggcacgagt aacaagctca
     1801 cgcagttggg cacttttgaa gatcattttc tcagcctcca gaggatgttc aataactgtg
     1861 aggtggtcct tgggaatttg gaaattacct atgtgcagag gaattatgat ctttccttct
     1921 taaagaccat ccaggaggtg gctggttatg tcctcattgc cctcaacaca gtggagcgaa
     1981 ttcctttgga aaacctgcag atcatcagag gaaatatgta ctacgaaaat tcctatgcct
     2041 tagcagtctt atctaactat gatgcaaata aaaccggact gaaggagctg cccatgagaa
     2101 atttacagga aatcctgcat ggcgccgtgc ggttcagcaa caaccctgcc ctgtgcaacg
     2161 tggagagcat ccagtggcgg gacatagtca gcagtgactt tctcagcaac atgtcgatgg
     2221 acttccagaa ccacctgggc agctgccaaa agtgtgatcc aagctgtccc aatgggagct
     2281 gctggggtgc aggagaggag aactgccaga aactgaccaa aatcatctgt gcccagcagt
     2341 gctccgggcg ctgccgtggc aagtccccca gtgactgctg ccacaaccag tgtgctgcag
     2401 gctgcacagg cccccgggag agcgactgcc tggtctgccg caaattccga gacgaagcca
     2461 cgtgcaagga cacctgcccc ccactcatgc tctacaaccc caccacgtac cagatggatg
     2521 tgaaccccga gggcaaatac agctttggtg ccacctgcgt gaagaagtgt ccccgtaatt
     2581 atgtggtgac agatcacggc tcgtgcgtcc gagcctgtgg ggccgacagc tatgagatgg
     2641 aggaagacgg cgtccgcaag tgtaagaagt gcgaagggcc ttgccgcaaa gtgtgtaacg
     2701 gaataggtat tggtgaattt aaagactcac tctccataaa tgctacgaat attaaacact
     2761 tcaaaaactg cacctccatc agtggcgatc tccacatcct gccggtggca tttaggggtg
     2821 actccttcac acatactcct cctctggatc cacaggaact ggatattctg aaaaccgtaa
     2881 aggaaatcac agggtttttg ctgattcagg cttggcctga aaacaggacg gacctccatg
     2941 cctttgagaa cctagaaatc atacgcggca ggaccaagca acatggtcag ttttctcttg
     3001 cagtcgtcag cctgaacata acatccttgg gattacgctc cctcaaggag ataagtgatg
     3061 gagatgtgat aatttcagga aacaaaaatt tgtgctatgc aaatacaata aactggaaaa
     3121 aactgtttgg gacctccggt cagaaaacca aaattataag caacagaggt gaaaacagct
     3181 gcaaggccac aggccaggtc tgccatgcct tgtgctcccc cgagggctgc tggggcccgg
     3241 agcccaggga ctgcgtctct tgccggaatg tcagccgagg cagggaatgc gtggacaagt
     3301 gcaaccttct ggagggtgag ccaagggagt ttgtggagaa ctctgagtgc atacagtgcc
     3361 acccagagtg cctgcctcag gccatgaaca tcacctgcac aggacgggga ccagacaact
     3421 gtatccagtg tgcccactac attgacggcc cccactgcgt caagacctgc ccggcaggag
     3481 tcatgggaga aaacaacacc ctggtctgga agtacgcaga cgccggccat gtgtgccacc
     3541 tgtgccatcc aaactgcacc tacggatgca ctgggccagg tcttgaaggc tgtccaacga
     3601 atgggcctaa gatcccgtcc atcgccactg ggatggtggg ggccctcctc ttgctgctgg
     3661 tggtggccct ggggatcggc ctcttcatgc gaaggcgcca catcgttcgg aagcgcacgc
     3721 tgcggaggct gctgcaggag agggagcttg tggagcctct tacacccagt ggagaagctc
     3781 ccaaccaagc tctcttgagg atcttgaagg aaactgaatt caaaaagatc aaagtgctgg
     3841 cctccggtgc gttcggcacg gtgtataagg gactctggat cccagaaggt gagaaagtta
     3901 aaattcccgt cgctatcaag gaattaagag aagcaacatc tccgaaagcc aacaaggaaa
     3961 tcctcgatga agcctacgtg atggccagcg tggacaaccc ccacgtgtgc cgcctgctgg
     4021 gcatctgcct cacctccacc gtgcagctca tcacgcagct catgcccttc ggctgcctcc
     4081 tggactatgt ccgggaacac aaagacaata ttggctccca gtacctgctc aactggtgtg
     4141 tgcagatcgc aaagggcatg aactacttgg aggaccgtcg cttggtgcac cgcgacctgg
     4201 cagccaggaa cgtactggtg aaaacaccgc agcatgtcaa gatcacagat tttgggctgg
     4261 ccaaactgct gggtgcggaa gagaaagaat accatgcaga aggaggcaaa gtgcctatca
     4321 agtggatggc attggaatca attttacaca gaatctatac ccaccagagt gatgtctgga
     4381 gctacggggt gaccgtttgg gagttgatga cctttggatc caagccatat gacggaatcc
     4441 ctgccagcga gatctcctcc atcctggaga aaggagaacg cctccctcag ccacccatat
     4501 gtaccatcga tgtctacatg atcatggtca agtgctggat gatagacgca gatagtcgcc
     4561 caaagttccg tgagttgatc atcgaattct ccaaaatggc ccgagacccc cagcgctacc
     4621 ttgtcattca gggggatgaa agaatgcatt tgccaagtcc tacagactcc aacttctacc
     4681 gtgccctgat ggatgaagaa gacatggacg acgtggtgga tgccgacgag tacctcatcc
     4741 cacagcaggg cttcttcagc agcccctcca cgtcacggac tcccctcctg agctctctga
     4801 gtgcaaccag caacaattcc accgtggctt gcattgatag aaatgggctg caaagctgtc
     4861 ccatcaagga agacagcttc ttgcagcgat acagctcaga ccccacaggc gccttgactg
     4921 aggacagcat agacgacacc ttcctcccag tgcctgaata cataaaccag tccgttccca
     4981 aaaggcccgc tggctctgtg cagaatcctg tctatcacaa tcagcctctg aaccccgcgc
     5041 ccagcagaga cccacactac caggaccccc acagcactgc agtgggcaac cccgagtatc
     5101 tcaacactgt ccagcccacc tgtgtcaaca gcacattcga cagccctgcc cactgggccc
     5161 agaaaggcag ccaccaaatt agcctggaca accctgacta ccagcaggac ttctttccca
     5221 aggaagccaa gccaaatggc atctttaagg gctccacagc tgaaaatgca gaatacctaa
     5281 gggtcgcgcc acaaagcagt gaatttattg gagcatagaa cccagctttc ttgtacaaag
     5341 tggtgatatc caattaaccc tcactaaagg tgtatatgtt aatgagatgt tgtaccctat
     5401 agtgagtcgt attaccaccc aacttttcta tacaaagtgg ttgatatcca gcacagtggc
     5461 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     5521 ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact
     5581 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     5641 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     5701 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     5761 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     5821 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     5881 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     5941 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     6001 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     6061 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     6121 taatatggcc acacatatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     6181 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     6241 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     6301 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     6361 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     6421 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     6481 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     6541 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     6601 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     6661 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     6721 caagcccggt gcctaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa
     6781 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6841 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6901 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6961 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7021 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7081 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7141 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7201 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7261 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7321 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga
     7381 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     7441 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     7501 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7561 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7621 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     7681 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc
     7741 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     7801 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7861 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     7921 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     7981 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8041 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8101 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8161 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     8221 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8281 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8341 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8401 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8461 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8521 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8581 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8641 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8701 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8761 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8821 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     8881 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     8941 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     9001 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     9061 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     9121 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     9181 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     9241 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     9301 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     9361 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     9421 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     9481 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     9541 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     9601 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     9661 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     9721 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     9781 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
     9841 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9901 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
     9961 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    10021 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    10081 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
    10141 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    10201 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    10261 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    10321 gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc
    10381 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
    10441 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
    10501 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
    10561 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg
    10621 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
    10681 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
    10741 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct
    10801 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    10861 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    10921 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    10981 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
    11041 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
    11101 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
    11161 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
    11221 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
    11281 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
    11341 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
    11401 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
    11461 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
    11521 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
    11581 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
    11641 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
    11701 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
    11761 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
    11821 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
    11881 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
    11941 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
    12001 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    12061 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    12121 gt
//
