LOCUS       sequence-250546-       10788 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF A762V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10788)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-A762V
FEATURES             Location/Qualifiers
     source          1..10788
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1199..1223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1224..1233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3526..3550)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3558..3576
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3601..3620)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3602..3620)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3626..3646)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3700..3741
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3762..4329
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3779..4331
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3923..3940)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4150..4169
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4343..4942
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4343..4362)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4839..4859
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4956..5544
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5009..5029)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5427..5438)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5619..5852
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5971..5989)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6057..6161
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6162..6230,6231..7022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6380..6399)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7193..7781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7682..7701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7935..7952
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8192..8213
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8228..8233,8234..8251,8252..8258)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8266..8282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8271..8293
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8290..8306
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8290..8306
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8356..8375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8567..9200
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9247..9372
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9869..10102
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10287..10331
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10624..10741
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa gcggccctgc agatatcaac
     1201 aagtttgtac aaaaaagcag gcaccatggc ggcgctgagc ggtggcggtg gtggcggcgc
     1261 ggagccgggc caggctctgt tcaacgggga catggagccc gaggccggcg ccggcgccgg
     1321 cgccgcggcc tcttcggctg cggaccctgc cattccggag gaggtgtgga atatcaaaca
     1381 aatgattaag ttgacacagg aacatataga ggccctattg gacaaatttg gtggggagca
     1441 taatccacca tcaatatatc tggaggccta tgaagaatac accagcaagc tagatgcact
     1501 ccaacaaaga gaacaacagt tattggaatc tctggggaac ggaactgatt tttctgtttc
     1561 tagctctgca tcaatggata ccgttacatc ttcttcctct tctagccttt cagtgctacc
     1621 ttcatctctt tcagtttttc aaaatcccac agatgtggca cggagcaacc ccaagtcacc
     1681 acaaaaacct atcgttagag tcttcctgcc caacaaacag aggacagtgg tacctgcaag
     1741 gtgtggagtt acagtccgag acagtctaaa gaaagcactg atgatgagag gtctaatccc
     1801 agagtgctgt gctgtttaca gaattcagga tggagagaag aaaccaattg gttgggacac
     1861 tgatatttcc tggcttactg gagaagaatt gcatgtggaa gtgttggaga atgttccact
     1921 tacaacacac aactttgtac gaaaaacgtt tttcacctta gcattttgtg acttttgtcg
     1981 aaagctgctt ttccagggtt tccgctgtca aacatgtggt tataaatttc accagcgttg
     2041 tagtacagaa gttccactga tgtgtgttaa ttatgaccaa cttgatttgc tgtttgtctc
     2101 caagttcttt gaacaccacc caataccaca ggaagaggcg tccttagcag agactgccct
     2161 aacatctgga tcatcccctt ccgcacccgc ctcggactct attgggcccc aaattctcac
     2221 cagtccgtct ccttcaaaat ccattccaat tccacagccc ttccgaccag cagatgaaga
     2281 tcatcgaaat caatttgggc aacgagaccg atcctcatca gctcccaatg tgcatataaa
     2341 cacaatagaa cctgtcaata ttgatgactt gattagagac caaggatttc gtggtgatgg
     2401 aggatcaacc acaggtttgt ctgctacccc ccctgcctca ttacctggct cactaactaa
     2461 cgtgaaagcc ttacagaaat ctccaggacc tcagcgagaa aggaagtcat cttcatcctc
     2521 agaagacagg aatcgaatga aaacacttgg tagacgggac tcgagtgatg attgggagat
     2581 tcctgatggg cagattacag tgggacaaag aattggatct ggatcatttg gaacagtcta
     2641 caagggaaag tggcatggtg atgtggcagt gaaaatgttg aatgtgacag cacctacacc
     2701 tcagcagtta caagccttca aaaatgaagt aggagtactc aggaaaacac gacatgtgaa
     2761 tatcctactc ttcatgggct attccacaaa gccacaactg gctattgtta cccagtggtg
     2821 tgagggctcc agcttgtatc accatctcca tatcattgag accaaatttg agatgatcaa
     2881 acttatagat attgcacgac agactgcaca gggcatggat tacttacacg ccaagtcaat
     2941 catccacaga gacctcaaga gtaataatat atttcttcat gaagacctca cagtaaaaat
     3001 aggtgatttt ggtctagcta cagtgaaatc tcgatggagt gggtcccatc agtttgaaca
     3061 gttgtctgga tccattttgt ggatggcacc agaagtcatc agaatgcaag ataaaaatcc
     3121 atacagcttt cagtcagatg tatatgcatt tggaattgtt ctgtatgaat tgatgactgg
     3181 acagttacct tattcaaaca tcaacaacag ggaccagata atttttatgg tgggacgagg
     3241 atacctgtct ccagatctca gtaaggtacg gagtaactgt ccaaaagcca tgaagagatt
     3301 aatggcagag tgcctcaaaa agaaaagaga tgagagacca ctctttcccc aaattctcgc
     3361 ctctattgag ctgctggccc gctcattgcc aaaaattcac cgcagtgcat cagaaccctc
     3421 cttgaatcgg gctggtttcc aaacagagga ttttagtcta tatgcttgtg cttctccaaa
     3481 aacacccatc caggcagggg gatatggtgt gtttcctgtc cactgaccca actttcttgt
     3541 acaaagtggt gatatccaat taaccctcac taaaggtagt tgaagttagt ttaagtgtta
     3601 ccctatagtg agtcgtatta ccacccaact tttctataca aagtggttga tatccagcac
     3661 agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     3721 ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc cccccctaac
     3781 gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc
     3841 accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg
     3901 agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg
     3961 aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc
     4021 aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa
     4081 gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa
     4141 agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta
     4201 ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg
     4261 aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca
     4321 cgatgataat atggccacac atatgaccga gtacaagccc acggtgcgcc tcgccacccg
     4381 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     4441 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     4501 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     4561 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     4621 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     4681 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     4741 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     4801 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4861 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4921 gacccgcaag cccggtgcct aaatcgatag atcctaatca acctctggat tacaaaattt
     4981 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5041 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5101 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     5161 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     5221 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     5281 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     5341 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     5401 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     5461 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     5521 tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt acaaggcagc
     5581 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5641 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     5701 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     5761 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     5821 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     5881 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt taattaagga
     5941 aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6001 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6061 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6121 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6181 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6241 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6301 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6361 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
     6421 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6481 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6541 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6601 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6661 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6721 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6781 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6841 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     6901 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6961 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7021 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7081 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7141 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7201 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7261 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7321 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7381 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7441 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7501 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7561 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7621 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7681 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7741 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7801 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     7861 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     7921 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7981 cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct gactaatttt
     8041 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8101 aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac aggtttcccg
     8161 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8221 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     8281 aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa gcattttttt
     8341 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     8401 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     8461 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     8521 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     8581 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     8641 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     8701 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     8761 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     8821 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     8881 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     8941 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     9001 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     9061 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9121 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9181 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     9241 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     9301 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     9361 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     9421 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     9481 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     9541 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     9601 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     9661 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     9721 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     9781 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     9841 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     9901 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     9961 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10021 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10081 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10141 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
    10201 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10261 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10321 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10381 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
    10441 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
    10501 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    10561 ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag tattcatcca
    10621 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
    10681 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
    10741 tcgggtttat tacagggaca gcagagatcc agtttggact agtcgtga
//
