LOCUS       sequence-250550-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR G719D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-G719D
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             25..66
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    87..654
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    104..656
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(248..265)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     475..494
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             668..1267
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(668..687)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1164..1184
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1281..1869
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1334..1354)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1752..1763)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1944..2177
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2296..2314)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2382..2486
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2487..2555,2556..3347)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3518..4106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4007..4026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4260..4277
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4517..4538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4553..4558,4559..4576,4577..4583)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4591..4607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4596..4618
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4615..4631
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4615..4631
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4681..4700
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4892..5525
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5572..5697
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6194..6427
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6612..6656
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6949..7066
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7114..8292
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7345..8283
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8240..8260
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    8312..8336
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(8339..8410,8411..10273,10274..10342,10343..11971)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLDSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(11973..11997)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        12005..12023
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12048..12067)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12049..12067)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(12073..12093)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
       61 tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc
      121 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
      181 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
      241 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
      301 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
      361 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
      421 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
      481 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
      541 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
      601 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
      661 cacacatatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
      721 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
      781 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
      841 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
      901 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
      961 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     1021 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     1081 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     1141 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     1201 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     1261 tgcctaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     1321 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     1381 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     1441 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     1501 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     1561 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     1621 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     1681 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     1741 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     1801 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     1861 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     1921 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     1981 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     2041 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     2101 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     2161 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     2221 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     2281 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     2341 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2401 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2461 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2521 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2581 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2641 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2701 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     2761 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2821 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2881 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2941 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3001 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3061 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3121 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3181 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3241 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     3301 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3361 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3421 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     4081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     4141 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     4201 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     4261 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     4321 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
     4381 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4441 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
     4501 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4561 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4621 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
     4681 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     4741 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     4801 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     4861 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
     4921 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     4981 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     5041 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     5101 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     5161 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     5221 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     5281 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     5341 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     5401 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     5461 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     5521 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     5581 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     5641 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     5701 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     5761 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     5821 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     5881 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     5941 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     6001 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     6061 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     6121 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     6181 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     6241 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     6301 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     6361 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     6421 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     6481 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     6541 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     6601 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     6661 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     6721 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     6781 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     6841 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     6901 gatctcgacg gtatcgccga attcacaaat ggcagtattc atccacaatt ttaaaagaaa
     6961 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     7021 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     7081 ggacagcaga gatccagttt ggactagtcg tgaggctccg gtgcccgtca gtgggcagag
     7141 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     7201 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     7261 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     7321 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     7381 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     7441 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     7501 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     7561 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     7621 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     7681 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     7741 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     7801 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     7861 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     7921 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     7981 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     8041 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     8101 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     8161 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     8221 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     8281 caggtgtcgt gaagcggccc tgcagatatc aacaagtttg tacaaaaaag caggcaccat
     8341 gcgaccctcc gggacggccg gggcagcgct cctggcgctg ctggctgcgc tctgcccggc
     8401 gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg agtaacaagc tcacgcagtt
     8461 gggcactttt gaagatcatt ttctcagcct ccagaggatg ttcaataact gtgaggtggt
     8521 ccttgggaat ttggaaatta cctatgtgca gaggaattat gatctttcct tcttaaagac
     8581 catccaggag gtggctggtt atgtcctcat tgccctcaac acagtggagc gaattccttt
     8641 ggaaaacctg cagatcatca gaggaaatat gtactacgaa aattcctatg ccttagcagt
     8701 cttatctaac tatgatgcaa ataaaaccgg actgaaggag ctgcccatga gaaatttaca
     8761 ggaaatcctg catggcgccg tgcggttcag caacaaccct gccctgtgca acgtggagag
     8821 catccagtgg cgggacatag tcagcagtga ctttctcagc aacatgtcga tggacttcca
     8881 gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt cccaatggga gctgctgggg
     8941 tgcaggagag gagaactgcc agaaactgac caaaatcatc tgtgcccagc agtgctccgg
     9001 gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac cagtgtgctg caggctgcac
     9061 aggcccccgg gagagcgact gcctggtctg ccgcaaattc cgagacgaag ccacgtgcaa
     9121 ggacacctgc cccccactca tgctctacaa ccccaccacg taccagatgg atgtgaaccc
     9181 cgagggcaaa tacagctttg gtgccacctg cgtgaagaag tgtccccgta attatgtggt
     9241 gacagatcac ggctcgtgcg tccgagcctg tggggccgac agctatgaga tggaggaaga
     9301 cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc aaagtgtgta acggaatagg
     9361 tattggtgaa tttaaagact cactctccat aaatgctacg aatattaaac acttcaaaaa
     9421 ctgcacctcc atcagtggcg atctccacat cctgccggtg gcatttaggg gtgactcctt
     9481 cacacatact cctcctctgg atccacagga actggatatt ctgaaaaccg taaaggaaat
     9541 cacagggttt ttgctgattc aggcttggcc tgaaaacagg acggacctcc atgcctttga
     9601 gaacctagaa atcatacgcg gcaggaccaa gcaacatggt cagttttctc ttgcagtcgt
     9661 cagcctgaac ataacatcct tgggattacg ctccctcaag gagataagtg atggagatgt
     9721 gataatttca ggaaacaaaa atttgtgcta tgcaaataca ataaactgga aaaaactgtt
     9781 tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga ggtgaaaaca gctgcaaggc
     9841 cacaggccag gtctgccatg ccttgtgctc ccccgagggc tgctggggcc cggagcccag
     9901 ggactgcgtc tcttgccgga atgtcagccg aggcagggaa tgcgtggaca agtgcaacct
     9961 tctggagggt gagccaaggg agtttgtgga gaactctgag tgcatacagt gccacccaga
    10021 gtgcctgcct caggccatga acatcacctg cacaggacgg ggaccagaca actgtatcca
    10081 gtgtgcccac tacattgacg gcccccactg cgtcaagacc tgcccggcag gagtcatggg
    10141 agaaaacaac accctggtct ggaagtacgc agacgccggc catgtgtgcc acctgtgcca
    10201 tccaaactgc acctacggat gcactgggcc aggtcttgaa ggctgtccaa cgaatgggcc
    10261 taagatcccg tccatcgcca ctgggatggt gggggccctc ctcttgctgc tggtggtggc
    10321 cctggggatc ggcctcttca tgcgaaggcg ccacatcgtt cggaagcgca cgctgcggag
    10381 gctgctgcag gagagggagc ttgtggagcc tcttacaccc agtggagaag ctcccaacca
    10441 agctctcttg aggatcttga aggaaactga attcaaaaag atcaaagtgc tggactccgg
    10501 tgcgttcggc acggtgtata agggactctg gatcccagaa ggtgagaaag ttaaaattcc
    10561 cgtcgctatc aaggaattaa gagaagcaac atctccgaaa gccaacaagg aaatcctcga
    10621 tgaagcctac gtgatggcca gcgtggacaa cccccacgtg tgccgcctgc tgggcatctg
    10681 cctcacctcc accgtgcagc tcatcacgca gctcatgccc ttcggctgcc tcctggacta
    10741 tgtccgggaa cacaaagaca atattggctc ccagtacctg ctcaactggt gtgtgcagat
    10801 cgcaaagggc atgaactact tggaggaccg tcgcttggtg caccgcgacc tggcagccag
    10861 gaacgtactg gtgaaaacac cgcagcatgt caagatcaca gattttgggc tggccaaact
    10921 gctgggtgcg gaagagaaag aataccatgc agaaggaggc aaagtgccta tcaagtggat
    10981 ggcattggaa tcaattttac acagaatcta tacccaccag agtgatgtct ggagctacgg
    11041 ggtgaccgtt tgggagttga tgacctttgg atccaagcca tatgacggaa tccctgccag
    11101 cgagatctcc tccatcctgg agaaaggaga acgcctccct cagccaccca tatgtaccat
    11161 cgatgtctac atgatcatgg tcaagtgctg gatgatagac gcagatagtc gcccaaagtt
    11221 ccgtgagttg atcatcgaat tctccaaaat ggcccgagac ccccagcgct accttgtcat
    11281 tcagggggat gaaagaatgc atttgccaag tcctacagac tccaacttct accgtgccct
    11341 gatggatgaa gaagacatgg acgacgtggt ggatgccgac gagtacctca tcccacagca
    11401 gggcttcttc agcagcccct ccacgtcacg gactcccctc ctgagctctc tgagtgcaac
    11461 cagcaacaat tccaccgtgg cttgcattga tagaaatggg ctgcaaagct gtcccatcaa
    11521 ggaagacagc ttcttgcagc gatacagctc agaccccaca ggcgccttga ctgaggacag
    11581 catagacgac accttcctcc cagtgcctga atacataaac cagtccgttc ccaaaaggcc
    11641 cgctggctct gtgcagaatc ctgtctatca caatcagcct ctgaaccccg cgcccagcag
    11701 agacccacac taccaggacc cccacagcac tgcagtgggc aaccccgagt atctcaacac
    11761 tgtccagccc acctgtgtca acagcacatt cgacagccct gcccactggg cccagaaagg
    11821 cagccaccaa attagcctgg acaaccctga ctaccagcag gacttctttc ccaaggaagc
    11881 caagccaaat ggcatcttta agggctccac agctgaaaat gcagaatacc taagggtcgc
    11941 gccacaaagc agtgaattta ttggagcata gaacccagct ttcttgtaca aagtggtgat
    12001 atccaattaa ccctcactaa aggagtaagt gttagatagt attgaatccc tatagtgagt
    12061 cgtattacca cccaactttt ctatacaaag tggttgatat ccagcacagt ggcggccgct
    12121 cg
//
