LOCUS       sequence-250560-       12280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ERBB2 D769H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12280)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB2-D769H
FEATURES             Location/Qualifiers
     source          1..12280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7217..7241)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7294..7335
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7356..7923
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7373..7925
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7517..7534)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7744..7763
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7937..7939,7940..7942,7943..8656)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7937..7939,7940..7942,7943..8653)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7982..8003)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8243..8262)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8590..8611
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8670..9258
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8723..8743)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9141..9152)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9333..9566
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9685..9703)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9771..9875
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9876..9944,9945..10736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10094..10113)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10907..11495
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11396..11415
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11649..11666
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11906..11927
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11942..11947,11948..11965,11966..11972)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11980..11996
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11985..12007
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12004..12020
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12004..12020
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12070..12089
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gagctggcgg ccttgtgccg ctgggggctc
     3481 ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg cacagacatg
     3541 aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca cctctaccag
     3601 ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa tgccagcctg
     3661 tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg
     3721 aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt tgaggacaac
     3781 tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc tgtcacaggg
     3841 gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat cttgaaagga
     3901 ggggtcttga tccagcggaa cccccagctc tgctaccagg acacgatttt gtggaaggac
     3961 atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg ctctcgggcc
     4021 tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag ttctgaggat
     4081 tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg
     4141 cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa gcactctgac
     4201 tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg cccagccctg
     4261 gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg gtatacattc
     4321 ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt gggatcctgc
     4381 accctcgtct gccccctgca caaccaagag gtgacagcag aggatggaac acagcggtgt
     4441 gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga gcacttgcga
     4501 gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa gaagatcttt
     4561 gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa cactgccccg
     4621 ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg ttacctatac
     4681 atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct gcaagtaatc
     4741 cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct gggcatcagc
     4801 tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat ccaccataac
     4861 acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa cccgcaccaa
     4921 gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg cctggcctgc
     4981 caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg tgtcaactgc
     5041 agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc
     5101 agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca gccccagaat
     5161 ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc ccactataag
     5221 gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct ctcctacatg
     5281 cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat caactgcacc
     5341 cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc cagccctctg
     5401 acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt
     5461 gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg gagactgctg
     5521 caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa ccaggcgcag
     5581 atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc tggcgctttt
     5641 ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat tccagtggcc
     5701 atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt acacgaagca
     5761 tacgtgatgg ctggtgtggg ctccccatat gtctcccgcc ttctgggcat ctgcctgaca
     5821 tccacggtgc agctggtgac acagcttatg ccctatggct gcctcttaga ccatgtccgg
     5881 gaaaaccgcg gacgcctggg ctcccaggac ctgctgaact ggtgtatgca gattgccaag
     5941 gggatgagct acctggagga tgtgcggctc gtacacaggg acttggccgc tcggaacgtg
     6001 ctggtcaaga gtcccaacca tgtcaaaatt acagacttcg ggctggctcg gctgctggac
     6061 attgacgaga cagagtacca tgcagatggg ggcaaggtgc ccatcaagtg gatggcgctg
     6121 gagtccattc tccgccggcg gttcacccac cagagtgatg tgtggagtta tggtgtgact
     6181 gtgtgggagc tgatgacttt tggggccaaa ccttacgatg ggatcccagc ccgggagatc
     6241 cctgacctgc tggaaaaggg ggagcggctg ccccagcccc ccatctgcac cattgatgtc
     6301 tacatgatca tggtcaaatg ttggatgatt gactctgaat gtcggccaag attccgggag
     6361 ttggtgtctg aattctcccg catggccagg gacccccagc gctttgtggt catccagaat
     6421 gaggacttgg gcccagccag tcccttggac agcaccttct accgctcact gctggaggac
     6481 gatgacatgg gggacctggt ggatgctgag gagtatctgg taccccagca gggcttcttc
     6541 tgtccagacc ctgccccggg cgctgggggc atggtccacc acaggcaccg cagctcatct
     6601 accaggagtg gcggtgggga cctgacacta gggctggagc cctctgaaga ggaggccccc
     6661 aggtctccac tggcaccctc cgaaggggct ggctccgatg tatttgatgg tgacctggga
     6721 atgggggcag ccaaggggct gcaaagcctc cccacacatg accccagccc tctacagcgg
     6781 tacagtgagg accccacagt acccctgccc tctgagactg atggctacgt tgcccccctg
     6841 acctgcagcc cccagcctga atatgtgaac cagccagatg ttcggcccca gcccccttcg
     6901 ccccgagagg gccctctgcc tgctgcccga cctgctggtg ccactctgga aaggcccaag
     6961 actctctccc cagggaagaa tggggtcgtc aaagacgttt ttgcctttgg gggtgccgtg
     7021 gagaaccccg agtacttgac accccaggga ggagctgccc ctcagcccca ccctcctcct
     7081 gccttcagcc cagccttcga caacctctat tactgggacc aggacccacc agagcggggg
     7141 gctccaccca gcaccttcaa agggacacct acggcagaga acccagagta cctgggtctg
     7201 gacgtgccag tgtagaaccc agctttcttg tacaaagtgg ttgatatcca gcacagtggc
     7261 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
     7321 ggtctcgatt ctacgcgtac cggttagtaa tgagatccct cccccccccc taacgttact
     7381 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata
     7441 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt
     7501 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa
     7561 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag
     7621 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca
     7681 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc
     7741 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat
     7801 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta
     7861 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga
     7921 taatatggcc acacatatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     7981 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     8041 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     8101 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     8161 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     8221 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     8281 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     8341 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     8401 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     8461 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     8521 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     8581 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     8641 cgagctgtac aagtaaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa
     8701 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     8761 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     8821 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     8881 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     8941 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     9001 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     9061 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     9121 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     9181 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     9241 tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga
     9301 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9361 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     9421 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9481 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9541 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     9601 ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc
     9661 tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     9721 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9781 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9841 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9901 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9961 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
    10021 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
    10081 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
    10141 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10201 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10261 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10321 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10381 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10441 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10501 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10561 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10621 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10681 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10741 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10801 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10861 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10921 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10981 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    11041 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    11101 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    11161 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11221 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11281 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11341 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11401 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11461 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11521 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11581 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11641 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11701 tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat
    11761 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    11821 ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga
    11881 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
    11941 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
    12001 acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc
    12061 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    12121 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    12181 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    12241 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
