LOCUS       sequence-250570-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR G810S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-G810S
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    195..312
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        360..1538
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          591..1529
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1486..1506
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1558..1582
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1585..1656,1657..3519,3520..3588,3589..5217)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNISSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5219..5243)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5251..5269
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5294..5313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5295..5313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5319..5339)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5393..5434
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5455..6022
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5472..6024
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5616..5633)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5843..5862
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6036..6635
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6036..6055)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6532..6552
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6649..7237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6702..6722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7120..7131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7312..7545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7664..7682)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7750..7854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7855..7923,7924..8715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8886..9474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9375..9394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9628..9645
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9885..9906
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9921..9926,9927..9944,9945..9951)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9959..9975
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9964..9986
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9983..9999
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9983..9999
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10049..10068
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10260..10893
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10940..11065
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11562..11795
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11980..12024
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
       61 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
      121 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca
      181 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      241 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      301 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac tagtcgtgag
      361 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      421 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      481 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      541 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      601 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      661 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
      721 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
      781 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
      841 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
      901 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
      961 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
     1021 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
     1081 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     1141 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     1201 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     1261 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     1321 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     1381 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     1441 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     1501 tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccctgca gatatcaaca
     1561 agtttgtaca aaaaagcagg caccatgcga ccctccggga cggccggggc agcgctcctg
     1621 gcgctgctgg ctgcgctctg cccggcgagt cgggctctgg aggaaaagaa agtttgccaa
     1681 ggcacgagta acaagctcac gcagttgggc acttttgaag atcattttct cagcctccag
     1741 aggatgttca ataactgtga ggtggtcctt gggaatttgg aaattaccta tgtgcagagg
     1801 aattatgatc tttccttctt aaagaccatc caggaggtgg ctggttatgt cctcattgcc
     1861 ctcaacacag tggagcgaat tcctttggaa aacctgcaga tcatcagagg aaatatgtac
     1921 tacgaaaatt cctatgcctt agcagtctta tctaactatg atgcaaataa aaccggactg
     1981 aaggagctgc ccatgagaaa tttacaggaa atcctgcatg gcgccgtgcg gttcagcaac
     2041 aaccctgccc tgtgcaacgt ggagagcatc cagtggcggg acatagtcag cagtgacttt
     2101 ctcagcaaca tgtcgatgga cttccagaac cacctgggca gctgccaaaa gtgtgatcca
     2161 agctgtccca atgggagctg ctggggtgca ggagaggaga actgccagaa actgaccaaa
     2221 atcatctgtg cccagcagtg ctccgggcgc tgccgtggca agtcccccag tgactgctgc
     2281 cacaaccagt gtgctgcagg ctgcacaggc ccccgggaga gcgactgcct ggtctgccgc
     2341 aaattccgag acgaagccac gtgcaaggac acctgccccc cactcatgct ctacaacccc
     2401 accacgtacc agatggatgt gaaccccgag ggcaaataca gctttggtgc cacctgcgtg
     2461 aagaagtgtc cccgtaatta tgtggtgaca gatcacggct cgtgcgtccg agcctgtggg
     2521 gccgacagct atgagatgga ggaagacggc gtccgcaagt gtaagaagtg cgaagggcct
     2581 tgccgcaaag tgtgtaacgg aataggtatt ggtgaattta aagactcact ctccataaat
     2641 gctacgaata ttaaacactt caaaaactgc acctccatca gtggcgatct ccacatcctg
     2701 ccggtggcat ttaggggtga ctccttcaca catactcctc ctctggatcc acaggaactg
     2761 gatattctga aaaccgtaaa ggaaatcaca gggtttttgc tgattcaggc ttggcctgaa
     2821 aacaggacgg acctccatgc ctttgagaac ctagaaatca tacgcggcag gaccaagcaa
     2881 catggtcagt tttctcttgc agtcgtcagc ctgaacataa catccttggg attacgctcc
     2941 ctcaaggaga taagtgatgg agatgtgata atttcaggaa acaaaaattt gtgctatgca
     3001 aatacaataa actggaaaaa actgtttggg acctccggtc agaaaaccaa aattataagc
     3061 aacagaggtg aaaacagctg caaggccaca ggccaggtct gccatgcctt gtgctccccc
     3121 gagggctgct ggggcccgga gcccagggac tgcgtctctt gccggaatgt cagccgaggc
     3181 agggaatgcg tggacaagtg caaccttctg gagggtgagc caagggagtt tgtggagaac
     3241 tctgagtgca tacagtgcca cccagagtgc ctgcctcagg ccatgaacat cacctgcaca
     3301 ggacggggac cagacaactg tatccagtgt gcccactaca ttgacggccc ccactgcgtc
     3361 aagacctgcc cggcaggagt catgggagaa aacaacaccc tggtctggaa gtacgcagac
     3421 gccggccatg tgtgccacct gtgccatcca aactgcacct acggatgcac tgggccaggt
     3481 cttgaaggct gtccaacgaa tgggcctaag atcccgtcca tcgccactgg gatggtgggg
     3541 gccctcctct tgctgctggt ggtggccctg gggatcggcc tcttcatgcg aaggcgccac
     3601 atcgttcgga agcgcacgct gcggaggctg ctgcaggaga gggagcttgt ggagcctctt
     3661 acacccagtg gagaagctcc caaccaagct ctcttgagga tcttgaagga aactgaattc
     3721 aaaaagatca aagtgctggg ctccggtgcg ttcggcacgg tgtataaggg actctggatc
     3781 ccagaaggtg agaaagttaa aattcccgtc gctatcaagg aattaagaga agcaacatct
     3841 ccgaaagcca acaaggaaat cctcgatgaa gcctacgtga tggccagcgt ggacaacccc
     3901 cacgtgtgcc gcctgctggg catctgcctc acctccaccg tgcagctcat cacgcagctc
     3961 atgcccttcg gctgcctcct ggactatgtc cgggaacaca aagacaatat tagctcccag
     4021 tacctgctca actggtgtgt gcagatcgca aagggcatga actacttgga ggaccgtcgc
     4081 ttggtgcacc gcgacctggc agccaggaac gtactggtga aaacaccgca gcatgtcaag
     4141 atcacagatt ttgggctggc caaactgctg ggtgcggaag agaaagaata ccatgcagaa
     4201 ggaggcaaag tgcctatcaa gtggatggca ttggaatcaa ttttacacag aatctatacc
     4261 caccagagtg atgtctggag ctacggggtg accgtttggg agttgatgac ctttggatcc
     4321 aagccatatg acggaatccc tgccagcgag atctcctcca tcctggagaa aggagaacgc
     4381 ctccctcagc cacccatatg taccatcgat gtctacatga tcatggtcaa gtgctggatg
     4441 atagacgcag atagtcgccc aaagttccgt gagttgatca tcgaattctc caaaatggcc
     4501 cgagaccccc agcgctacct tgtcattcag ggggatgaaa gaatgcattt gccaagtcct
     4561 acagactcca acttctaccg tgccctgatg gatgaagaag acatggacga cgtggtggat
     4621 gccgacgagt acctcatccc acagcagggc ttcttcagca gcccctccac gtcacggact
     4681 cccctcctga gctctctgag tgcaaccagc aacaattcca ccgtggcttg cattgataga
     4741 aatgggctgc aaagctgtcc catcaaggaa gacagcttct tgcagcgata cagctcagac
     4801 cccacaggcg ccttgactga ggacagcata gacgacacct tcctcccagt gcctgaatac
     4861 ataaaccagt ccgttcccaa aaggcccgct ggctctgtgc agaatcctgt ctatcacaat
     4921 cagcctctga accccgcgcc cagcagagac ccacactacc aggaccccca cagcactgca
     4981 gtgggcaacc ccgagtatct caacactgtc cagcccacct gtgtcaacag cacattcgac
     5041 agccctgccc actgggccca gaaaggcagc caccaaatta gcctggacaa ccctgactac
     5101 cagcaggact tctttcccaa ggaagccaag ccaaatggca tctttaaggg ctccacagct
     5161 gaaaatgcag aatacctaag ggtcgcgcca caaagcagtg aatttattgg agcatagaac
     5221 ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaaggt atgtgtatga
     5281 agattatagt tagccctata gtgagtcgta ttaccaccca acttttctat acaaagtggt
     5341 tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     5401 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat gagatccctc
     5461 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     5521 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     5581 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     5641 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     5701 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     5761 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     5821 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     5881 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     5941 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     6001 cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag cccacggtgc
     6061 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     6121 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     6181 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6241 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6301 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6361 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     6421 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6481 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6541 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6601 gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa tcaacctctg
     6661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     6721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     6781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     6841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     6901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     6961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     7021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     7081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     7141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     7201 cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta agaccaatga
     7261 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7321 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7381 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7441 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7501 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7561 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcccg
     7621 ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt gatacgccta
     7681 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     8101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     9601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     9661 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag caagctcatg
     9721 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     9781 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc cccgtggcac
     9841 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     9901 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     9961 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa tttcacaaat
    10021 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10081 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    10141 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    10201 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
    10261 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
    10321 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
    10381 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
    10441 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
    10501 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
    10561 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
    10621 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
    10681 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
    10741 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    10801 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10861 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
    10921 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
    10981 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
    11041 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
    11101 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
    11161 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
    11221 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
    11281 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
    11341 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
    11401 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
    11461 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    11521 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    11581 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    11641 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    11701 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    11761 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    11821 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    11881 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    11941 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    12001 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    12061 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    12121 ct
//
