LOCUS       sequence-250575-       12260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ERBB2 G776delinsVC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12260)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB2-G776delinsVC
FEATURES             Location/Qualifiers
     source          1..12260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7220..7244)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7252..7270
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7295..7314)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7296..7314)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7320..7340)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7394..7435
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7456..8023
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7473..8025
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7617..7634)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7844..7863
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8037..8636
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8037..8056)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8533..8553
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8650..9238
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8703..8723)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9121..9132)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9313..9546
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9665..9683)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9751..9855
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9856..9924,9925..10716)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10074..10093)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10887..11475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11376..11395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11629..11646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11886..11907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11922..11927,11928..11945,11946..11952)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11960..11976
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11965..11987
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11984..12000
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11984..12000
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12050..12069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gagctggcgg ccttgtgccg ctgggggctc
     3481 ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg cacagacatg
     3541 aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca cctctaccag
     3601 ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa tgccagcctg
     3661 tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg
     3721 aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt tgaggacaac
     3781 tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc tgtcacaggg
     3841 gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat cttgaaagga
     3901 ggggtcttga tccagcggaa cccccagctc tgctaccagg acacgatttt gtggaaggac
     3961 atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg ctctcgggcc
     4021 tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag ttctgaggat
     4081 tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg
     4141 cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa gcactctgac
     4201 tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg cccagccctg
     4261 gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg gtatacattc
     4321 ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt gggatcctgc
     4381 accctcgtct gccccctgca caaccaagag gtgacagcag aggatggaac acagcggtgt
     4441 gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga gcacttgcga
     4501 gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa gaagatcttt
     4561 gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa cactgccccg
     4621 ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg ttacctatac
     4681 atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct gcaagtaatc
     4741 cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct gggcatcagc
     4801 tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat ccaccataac
     4861 acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa cccgcaccaa
     4921 gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg cctggcctgc
     4981 caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg tgtcaactgc
     5041 agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc
     5101 agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca gccccagaat
     5161 ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc ccactataag
     5221 gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct ctcctacatg
     5281 cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat caactgcacc
     5341 cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc cagccctctg
     5401 acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt
     5461 gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg gagactgctg
     5521 caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa ccaggcgcag
     5581 atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc tggcgctttt
     5641 ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat tccagtggcc
     5701 atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt agacgaagca
     5761 tacgtgatgg ctgtatgtgt gggctcccca tatgtctccc gccttctggg catctgcctg
     5821 acatccacgg tgcagctggt gacacagctt atgccctatg gctgcctctt agaccatgtc
     5881 cgggaaaacc gcggacgcct gggctcccag gacctgctga actggtgtat gcagattgcc
     5941 aaggggatga gctacctgga ggatgtgcgg ctcgtacaca gggacttggc cgctcggaac
     6001 gtgctggtca agagtcccaa ccatgtcaaa attacagact tcgggctggc tcggctgctg
     6061 gacattgacg agacagagta ccatgcagat gggggcaagg tgcccatcaa gtggatggcg
     6121 ctggagtcca ttctccgccg gcggttcacc caccagagtg atgtgtggag ttatggtgtg
     6181 actgtgtggg agctgatgac ttttggggcc aaaccttacg atgggatccc agcccgggag
     6241 atccctgacc tgctggaaaa gggggagcgg ctgccccagc cccccatctg caccattgat
     6301 gtctacatga tcatggtcaa atgttggatg attgactctg aatgtcggcc aagattccgg
     6361 gagttggtgt ctgaattctc ccgcatggcc agggaccccc agcgctttgt ggtcatccag
     6421 aatgaggact tgggcccagc cagtcccttg gacagcacct tctaccgctc actgctggag
     6481 gacgatgaca tgggggacct ggtggatgct gaggagtatc tggtacccca gcagggcttc
     6541 ttctgtccag accctgcccc gggcgctggg ggcatggtcc accacaggca ccgcagctca
     6601 tctaccagga gtggcggtgg ggacctgaca ctagggctgg agccctctga agaggaggcc
     6661 cccaggtctc cactggcacc ctccgaaggg gctggctccg atgtatttga tggtgacctg
     6721 ggaatggggg cagccaaggg gctgcaaagc ctccccacac atgaccccag ccctctacag
     6781 cggtacagtg aggaccccac agtacccctg ccctctgaga ctgatggcta cgttgccccc
     6841 ctgacctgca gcccccagcc tgaatatgtg aaccagccag atgttcggcc ccagccccct
     6901 tcgccccgag agggccctct gcctgctgcc cgacctgctg gtgccactct ggaaaggccc
     6961 aagactctct ccccagggaa gaatggggtc gtcaaagacg tttttgcctt tgggggtgcc
     7021 gtggagaacc ccgagtactt gacaccccag ggaggagctg cccctcagcc ccaccctcct
     7081 cctgccttca gcccagcctt cgacaacctc tattactggg accaggaccc accagagcgg
     7141 ggggctccac ccagcacctt caaagggaca cctacggcag agaacccaga gtacctgggt
     7201 ctggacgtgc cagtgtagaa cccagctttc ttgtacaaag tggtgatatc caattaaccc
     7261 tcactaaagg tatttgataa gagaatgaag aagtccctat agtgagtcgt attaccaccc
     7321 aacttttcta tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtctagaggg
     7381 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     7441 cggttagtaa tgagatccct cccccccccc taacgttact ggccgaagcc gcttggaata
     7501 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     7561 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     7621 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     7681 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     7741 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     7801 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     7861 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     7921 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     7981 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acacatatga
     8041 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     8101 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     8161 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     8221 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     8281 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     8341 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     8401 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8461 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     8521 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     8581 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctaaatcg
     8641 atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     8701 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     8761 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     8821 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     8881 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     8941 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     9001 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     9061 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     9121 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     9181 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     9241 gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     9301 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     9361 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9421 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9481 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9541 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     9601 tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga
     9661 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     9721 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9781 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9841 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9901 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9961 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    10021 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    10081 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
    10141 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    10201 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    10261 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    10321 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    10381 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    10441 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    10501 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    10561 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    10621 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    10681 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    10741 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10801 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10861 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10921 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10981 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    11041 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    11101 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    11161 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    11221 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    11281 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    11341 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    11401 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    11461 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    11521 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    11581 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    11641 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    11701 cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    11761 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    11821 gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    11881 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    11941 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    12001 atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    12061 aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat
    12121 catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta
    12181 ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata
    12241 tgtactacaa acttagtagt
//
