LOCUS       sequence-250577-       12145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR H304Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-H304Y
FEATURES             Location/Qualifiers
     source          1..12145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             37..81
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    374..491
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        539..1717
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          770..1708
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1665..1685
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1737..1761
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1764..1835,1836..3698,3699..3767,3768..5396)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDYGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5398..5422)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5475..5516
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5537..6104
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5554..6106
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5698..5715)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5925..5944
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6118..6120,6121..6123,6124..6837)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6118..6120,6121..6123,6124..6834)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6163..6184)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6424..6443)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6771..6792
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6851..7439
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6904..6924)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7322..7333)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7514..7747
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7866..7884)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7952..8056
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8057..8125,8126..8917)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9088..9676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9577..9596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9830..9847
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10087..10108
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10123..10128,10129..10146,10147..10153)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10161..10177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10166..10188
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10185..10201
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10185..10201
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10251..10270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10462..11095
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11142..11267
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11764..11997
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
       61 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
      121 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
      181 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
      241 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
      301 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
      361 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
      421 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
      481 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggact agtcgtgagg
      541 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
      601 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
      661 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
      721 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
      781 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
      841 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
      901 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
      961 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     1021 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     1081 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     1141 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     1201 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     1261 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     1321 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     1381 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     1441 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     1501 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     1561 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     1621 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     1681 ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc ggccctgcag atatcaacaa
     1741 gtttgtacaa aaaagcaggc accatgcgac cctccgggac ggccggggca gcgctcctgg
     1801 cgctgctggc tgcgctctgc ccggcgagtc gggctctgga ggaaaagaaa gtttgccaag
     1861 gcacgagtaa caagctcacg cagttgggca cttttgaaga tcattttctc agcctccaga
     1921 ggatgttcaa taactgtgag gtggtccttg ggaatttgga aattacctat gtgcagagga
     1981 attatgatct ttccttctta aagaccatcc aggaggtggc tggttatgtc ctcattgccc
     2041 tcaacacagt ggagcgaatt cctttggaaa acctgcagat catcagagga aatatgtact
     2101 acgaaaattc ctatgcctta gcagtcttat ctaactatga tgcaaataaa accggactga
     2161 aggagctgcc catgagaaat ttacaggaaa tcctgcatgg cgccgtgcgg ttcagcaaca
     2221 accctgccct gtgcaacgtg gagagcatcc agtggcggga catagtcagc agtgactttc
     2281 tcagcaacat gtcgatggac ttccagaacc acctgggcag ctgccaaaag tgtgatccaa
     2341 gctgtcccaa tgggagctgc tggggtgcag gagaggagaa ctgccagaaa ctgaccaaaa
     2401 tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa gtcccccagt gactgctgcc
     2461 acaaccagtg tgctgcaggc tgcacaggcc cccgggagag cgactgcctg gtctgccgca
     2521 aattccgaga cgaagccacg tgcaaggaca cctgcccccc actcatgctc tacaacccca
     2581 ccacgtacca gatggatgtg aaccccgagg gcaaatacag ctttggtgcc acctgcgtga
     2641 agaagtgtcc ccgtaattat gtggtgacag attacggctc gtgcgtccga gcctgtgggg
     2701 ccgacagcta tgagatggag gaagacggcg tccgcaagtg taagaagtgc gaagggcctt
     2761 gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa agactcactc tccataaatg
     2821 ctacgaatat taaacacttc aaaaactgca cctccatcag tggcgatctc cacatcctgc
     2881 cggtggcatt taggggtgac tccttcacac atactcctcc tctggatcca caggaactgg
     2941 atattctgaa aaccgtaaag gaaatcacag ggtttttgct gattcaggct tggcctgaaa
     3001 acaggacgga cctccatgcc tttgagaacc tagaaatcat acgcggcagg accaagcaac
     3061 atggtcagtt ttctcttgca gtcgtcagcc tgaacataac atccttggga ttacgctccc
     3121 tcaaggagat aagtgatgga gatgtgataa tttcaggaaa caaaaatttg tgctatgcaa
     3181 atacaataaa ctggaaaaaa ctgtttggga cctccggtca gaaaaccaaa attataagca
     3241 acagaggtga aaacagctgc aaggccacag gccaggtctg ccatgccttg tgctcccccg
     3301 agggctgctg gggcccggag cccagggact gcgtctcttg ccggaatgtc agccgaggca
     3361 gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc aagggagttt gtggagaact
     3421 ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc catgaacatc acctgcacag
     3481 gacggggacc agacaactgt atccagtgtg cccactacat tgacggcccc cactgcgtca
     3541 agacctgccc ggcaggagtc atgggagaaa acaacaccct ggtctggaag tacgcagacg
     3601 ccggccatgt gtgccacctg tgccatccaa actgcaccta cggatgcact gggccaggtc
     3661 ttgaaggctg tccaacgaat gggcctaaga tcccgtccat cgccactggg atggtggggg
     3721 ccctcctctt gctgctggtg gtggccctgg ggatcggcct cttcatgcga aggcgccaca
     3781 tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag ggagcttgtg gagcctctta
     3841 cacccagtgg agaagctccc aaccaagctc tcttgaggat cttgaaggaa actgaattca
     3901 aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt gtataaggga ctctggatcc
     3961 cagaaggtga gaaagttaaa attcccgtcg ctatcaagga attaagagaa gcaacatctc
     4021 cgaaagccaa caaggaaatc ctcgatgaag cctacgtgat ggccagcgtg gacaaccccc
     4081 acgtgtgccg cctgctgggc atctgcctca cctccaccgt gcagctcatc acgcagctca
     4141 tgcccttcgg ctgcctcctg gactatgtcc gggaacacaa agacaatatt ggctcccagt
     4201 acctgctcaa ctggtgtgtg cagatcgcaa agggcatgaa ctacttggag gaccgtcgct
     4261 tggtgcaccg cgacctggca gccaggaacg tactggtgaa aacaccgcag catgtcaaga
     4321 tcacagattt tgggctggcc aaactgctgg gtgcggaaga gaaagaatac catgcagaag
     4381 gaggcaaagt gcctatcaag tggatggcat tggaatcaat tttacacaga atctataccc
     4441 accagagtga tgtctggagc tacggggtga ccgtttggga gttgatgacc tttggatcca
     4501 agccatatga cggaatccct gccagcgaga tctcctccat cctggagaaa ggagaacgcc
     4561 tccctcagcc acccatatgt accatcgatg tctacatgat catggtcaag tgctggatga
     4621 tagacgcaga tagtcgccca aagttccgtg agttgatcat cgaattctcc aaaatggccc
     4681 gagaccccca gcgctacctt gtcattcagg gggatgaaag aatgcatttg ccaagtccta
     4741 cagactccaa cttctaccgt gccctgatgg atgaagaaga catggacgac gtggtggatg
     4801 ccgacgagta cctcatccca cagcagggct tcttcagcag cccctccacg tcacggactc
     4861 ccctcctgag ctctctgagt gcaaccagca acaattccac cgtggcttgc attgatagaa
     4921 atgggctgca aagctgtccc atcaaggaag acagcttctt gcagcgatac agctcagacc
     4981 ccacaggcgc cttgactgag gacagcatag acgacacctt cctcccagtg cctgaataca
     5041 taaaccagtc cgttcccaaa aggcccgctg gctctgtgca gaatcctgtc tatcacaatc
     5101 agcctctgaa ccccgcgccc agcagagacc cacactacca ggacccccac agcactgcag
     5161 tgggcaaccc cgagtatctc aacactgtcc agcccacctg tgtcaacagc acattcgaca
     5221 gccctgccca ctgggcccag aaaggcagcc accaaattag cctggacaac cctgactacc
     5281 agcaggactt ctttcccaag gaagccaagc caaatggcat ctttaagggc tccacagctg
     5341 aaaatgcaga atacctaagg gtcgcgccac aaagcagtga atttattgga gcatagaacc
     5401 cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg
     5461 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     5521 ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     5581 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     5641 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     5701 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     5761 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     5821 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     5881 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     5941 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     6001 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     6061 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacacatatg
     6121 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     6181 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     6241 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     6301 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     6361 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     6421 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     6481 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     6541 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     6601 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     6661 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     6721 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     6781 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaatc
     6841 gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     6901 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     6961 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7021 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7081 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7141 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7201 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7261 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7321 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7381 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     7441 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7501 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     7561 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7621 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7681 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7741 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7801 atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt cttgaagacg
     7861 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     7921 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7981 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     8041 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8101 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8161 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8221 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     8281 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8341 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8401 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8461 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8521 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8581 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8641 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8701 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8761 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8821 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8881 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8941 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9001 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9061 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     9121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     9181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     9241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     9361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     9421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     9481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9721 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     9781 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     9841 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     9901 tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga ggccgaggcc
     9961 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
    10021 tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
    10081 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10141 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    10201 catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10261 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    10321 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
    10381 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
    10441 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
    10501 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
    10561 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
    10621 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
    10681 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
    10741 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
    10801 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
    10861 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
    10921 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10981 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    11041 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
    11101 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
    11161 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
    11221 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
    11281 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
    11341 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
    11401 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
    11461 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
    11521 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
    11581 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
    11641 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
    11701 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
    11761 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
    11821 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    11881 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    11941 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    12001 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    12061 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    12121 aattaacaat tacacaagct taata
//
