LOCUS       sequence-25058-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: Flag pLJM1 RagD wt
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..57
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccggt atggactaca aggatgacga tgacaagggt
       61 ggtggtggtg gtgcgtcgac gatgagccag gtgctgggga agccgcagcc gcaggacgag
      121 gacgacgcgg aggaggagga ggaggaggat gagctggtgg ggctagcgga ctacggagac
      181 gggcccgact cctccgacgc cgatccggac agcggcacag aggagggagt tctggacttc
      241 agtgacccct tcagcactga agtgaagccg agaatcctgc tcatgggcct gaggagaagc
      301 ggcaagtcgt ctattcagaa agttgtcttt cacaaaatgt ctcccaacga aactctgttc
      361 ttggagagca ctaataagat atgccgggaa gatgtttcca acagctcctt tgtcaatttt
      421 cagatttggg acttcccagg acagattgac ttttttgacc ctacatttga ctatgagatg
      481 atcttccggg gaacaggagc actgatattt gtcattgact cacaggatga ttacatggaa
      541 gccctggcca ggctccacct cacggtgacc agggcctaca aagtgaatac tgacatcaac
      601 ttcgaggtgt ttattcataa agtggatggt ctgtcagatg accacaaaat tgaaacccaa
      661 agagatattc accagagggc aaacgatgac cttgcagatg ctggatta
//
