LOCUS       sequence-250580-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF G464E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-G464E
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2349..2373)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2381..2399
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2424..2443)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2425..2443)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2449..2469)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             2523..2564
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2585..3152
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2602..3154
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2746..2763)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2973..2992
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3166..3765
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3166..3185)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3662..3682
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3779..4367
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3832..3852)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4250..4261)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4442..4675
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4794..4812)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4880..4984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4985..5053,5054..5845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5203..5222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6016..6604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6505..6524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6758..6775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7015..7036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7051..7056,7057..7074,7075..7081)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7089..7105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7094..7116
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7113..7129
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7113..7129
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7179..7198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7390..8023
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8070..8195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8692..8925
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9110..9154
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9447..9564
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9612..10790
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9843..10781
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10738..10758
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg cggcgctgag
       61 cggtggcggt ggtggcggcg cggagccggg ccaggctctg ttcaacgggg acatggagcc
      121 cgaggccggc gccggcgccg gcgccgcggc ctcttcggct gcggaccctg ccattccgga
      181 ggaggtgtgg aatatcaaac aaatgattaa gttgacacag gaacatatag aggccctatt
      241 ggacaaattt ggtggggagc ataatccacc atcaatatat ctggaggcct atgaagaata
      301 caccagcaag ctagatgcac tccaacaaag agaacaacag ttattggaat ctctggggaa
      361 cggaactgat ttttctgttt ctagctctgc atcaatggat accgttacat cttcttcctc
      421 ttctagcctt tcagtgctac cttcatctct ttcagttttt caaaatccca cagatgtggc
      481 acggagcaac cccaagtcac cacaaaaacc tatcgttaga gtcttcctgc ccaacaaaca
      541 gaggacagtg gtacctgcaa ggtgtggagt tacagtccga gacagtctaa agaaagcact
      601 gatgatgaga ggtctaatcc cagagtgctg tgctgtttac agaattcagg atggagagaa
      661 gaaaccaatt ggttgggaca ctgatatttc ctggcttact ggagaagaat tgcatgtgga
      721 agtgttggag aatgttccac ttacaacaca caactttgta cgaaaaacgt ttttcacctt
      781 agcattttgt gacttttgtc gaaagctgct tttccagggt ttccgctgtc aaacatgtgg
      841 ttataaattt caccagcgtt gtagtacaga agttccactg atgtgtgtta attatgacca
      901 acttgatttg ctgtttgtct ccaagttctt tgaacaccac ccaataccac aggaagaggc
      961 gtccttagca gagactgccc taacatctgg atcatcccct tccgcacccg cctcggactc
     1021 tattgggccc caaattctca ccagtccgtc tccttcaaaa tccattccaa ttccacagcc
     1081 cttccgacca gcagatgaag atcatcgaaa tcaatttggg caacgagacc gatcctcatc
     1141 agctcccaat gtgcatataa acacaataga acctgtcaat attgatgact tgattagaga
     1201 ccaaggattt cgtggtgatg gaggatcaac cacaggtttg tctgctaccc cccctgcctc
     1261 attacctggc tcactaacta acgtgaaagc cttacagaaa tctccaggac ctcagcgaga
     1321 aaggaagtca tcttcatcct cagaagacag gaatcgaatg aaaacacttg gtagacggga
     1381 ctcgagtgat gattgggaga ttcctgatgg gcagattaca gtgggacaaa gaattgaatc
     1441 tggatcattt ggaacagtct acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt
     1501 gaatgtgaca gcacctacac ctcagcagtt acaagccttc aaaaatgaag taggagtact
     1561 caggaaaaca cgacatgtga atatcctact cttcatgggc tattccacaa agccacaact
     1621 ggctattgtt acccagtggt gtgagggctc cagcttgtat caccatctcc atatcattga
     1681 gaccaaattt gagatgatca aacttataga tattgcacga cagactgcac agggcatgga
     1741 ttacttacac gccaagtcaa tcatccacag agacctcaag agtaataata tatttcttca
     1801 tgaagacctc acagtaaaaa taggtgattt tggtctagct acagtgaaat ctcgatggag
     1861 tgggtcccat cagtttgaac agttgtctgg atccattttg tggatggcac cagaagtcat
     1921 cagaatgcaa gataaaaatc catacagctt tcagtcagat gtatatgcat ttggaattgt
     1981 tctgtatgaa ttgatgactg gacagttacc ttattcaaac atcaacaaca gggaccagat
     2041 aatttttatg gtgggacgag gatacctgtc tccagatctc agtaaggtac ggagtaactg
     2101 tccaaaagcc atgaagagat taatggcaga gtgcctcaaa aagaaaagag atgagagacc
     2161 actctttccc caaattctcg cctctattga gctgctggcc cgctcattgc caaaaattca
     2221 ccgcagtgca tcagaaccct ccttgaatcg ggctggtttc caaacagagg attttagtct
     2281 atatgcttgt gcttctccaa aaacacccat ccaggcaggg ggatatggtg cgtttcctgt
     2341 ccactagaac ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaagga
     2401 atagataaga ttgattgtgt ttgccctata gtgagtcgta ttaccaccca acttttctat
     2461 acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
     2521 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
     2581 gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     2641 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     2701 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     2761 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     2821 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     2881 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     2941 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     3001 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     3061 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     3121 acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatgac cgagtacaag
     3181 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     3241 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     3301 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     3361 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     3421 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     3481 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     3541 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     3601 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     3661 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     3721 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctaaatcga tagatcctaa
     3781 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3841 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3901 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3961 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4021 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4081 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4141 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4201 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4261 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4321 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     4381 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4441 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4501 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4561 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4621 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     4681 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     4741 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     4801 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4921 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4981 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5101 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5161 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5221 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5281 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5341 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5401 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5521 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5581 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5641 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5701 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5761 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5821 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5881 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5941 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6001 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6061 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6841 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6901 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     6961 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7021 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7081 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     7141 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7201 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     7261 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     7321 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     7381 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     7441 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     7501 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     7561 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     7621 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     7681 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     7741 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     7801 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8041 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8101 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8161 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8221 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8281 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8341 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8401 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8461 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8521 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8581 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8641 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8701 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8761 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8821 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8881 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8941 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9001 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9061 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9121 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9181 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9241 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9301 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     9361 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
     9421 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     9481 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     9541 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     9601 actagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     9661 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     9721 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     9781 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     9841 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     9901 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     9961 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
    10021 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
    10081 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
    10141 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
    10201 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
    10261 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
    10321 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
    10381 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
    10441 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
    10501 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
    10561 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
    10621 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
    10681 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
    10741 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
//
