LOCUS       sequence-250582-       12145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR H47Y.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12145)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-H47Y
FEATURES             Location/Qualifiers
     source          1..12145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    75..192
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        240..1418
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          471..1409
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1366..1386
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1438..1462
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1465..1536,1537..3399,3400..3468,3469..5097)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DYFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5099..5123)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5176..5217
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5238..5805
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5255..5807
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5399..5416)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5626..5645
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5819..5821,5822..5824,5825..6538)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5819..5821,5822..5824,5825..6535)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5864..5885)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6125..6144)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6472..6493
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6552..7140
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6605..6625)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7023..7034)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7215..7448
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7567..7585)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7653..7757
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7758..7826,7827..8618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8789..9377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9278..9297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9531..9548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9788..9809
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9824..9829,9830..9847,9848..9854)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9862..9878
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9867..9889
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9886..9902
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9886..9902
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9952..9971
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10163..10796
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10843..10968
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11465..11698
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11883..11927
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agacagatcc attcgattag tgaacggatc tcgacggtat cgccgaattc acaaatggca
       61 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
      121 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
      181 attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggac tagtcgtgag
      241 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
      301 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      361 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      421 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      481 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      541 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
      601 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
      661 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
      721 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
      781 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
      841 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
      901 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
      961 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
     1021 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
     1081 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
     1141 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
     1201 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
     1261 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
     1321 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
     1381 tggttcaaag tttttttctt ccatttcagg tgtcgtgaag cggccctgca gatatcaaca
     1441 agtttgtaca aaaaagcagg caccatgcga ccctccggga cggccggggc agcgctcctg
     1501 gcgctgctgg ctgcgctctg cccggcgagt cgggctctgg aggaaaagaa agtttgccaa
     1561 ggcacgagta acaagctcac gcagttgggc acttttgaag attattttct cagcctccag
     1621 aggatgttca ataactgtga ggtggtcctt gggaatttgg aaattaccta tgtgcagagg
     1681 aattatgatc tttccttctt aaagaccatc caggaggtgg ctggttatgt cctcattgcc
     1741 ctcaacacag tggagcgaat tcctttggaa aacctgcaga tcatcagagg aaatatgtac
     1801 tacgaaaatt cctatgcctt agcagtctta tctaactatg atgcaaataa aaccggactg
     1861 aaggagctgc ccatgagaaa tttacaggaa atcctgcatg gcgccgtgcg gttcagcaac
     1921 aaccctgccc tgtgcaacgt ggagagcatc cagtggcggg acatagtcag cagtgacttt
     1981 ctcagcaaca tgtcgatgga cttccagaac cacctgggca gctgccaaaa gtgtgatcca
     2041 agctgtccca atgggagctg ctggggtgca ggagaggaga actgccagaa actgaccaaa
     2101 atcatctgtg cccagcagtg ctccgggcgc tgccgtggca agtcccccag tgactgctgc
     2161 cacaaccagt gtgctgcagg ctgcacaggc ccccgggaga gcgactgcct ggtctgccgc
     2221 aaattccgag acgaagccac gtgcaaggac acctgccccc cactcatgct ctacaacccc
     2281 accacgtacc agatggatgt gaaccccgag ggcaaataca gctttggtgc cacctgcgtg
     2341 aagaagtgtc cccgtaatta tgtggtgaca gatcacggct cgtgcgtccg agcctgtggg
     2401 gccgacagct atgagatgga ggaagacggc gtccgcaagt gtaagaagtg cgaagggcct
     2461 tgccgcaaag tgtgtaacgg aataggtatt ggtgaattta aagactcact ctccataaat
     2521 gctacgaata ttaaacactt caaaaactgc acctccatca gtggcgatct ccacatcctg
     2581 ccggtggcat ttaggggtga ctccttcaca catactcctc ctctggatcc acaggaactg
     2641 gatattctga aaaccgtaaa ggaaatcaca gggtttttgc tgattcaggc ttggcctgaa
     2701 aacaggacgg acctccatgc ctttgagaac ctagaaatca tacgcggcag gaccaagcaa
     2761 catggtcagt tttctcttgc agtcgtcagc ctgaacataa catccttggg attacgctcc
     2821 ctcaaggaga taagtgatgg agatgtgata atttcaggaa acaaaaattt gtgctatgca
     2881 aatacaataa actggaaaaa actgtttggg acctccggtc agaaaaccaa aattataagc
     2941 aacagaggtg aaaacagctg caaggccaca ggccaggtct gccatgcctt gtgctccccc
     3001 gagggctgct ggggcccgga gcccagggac tgcgtctctt gccggaatgt cagccgaggc
     3061 agggaatgcg tggacaagtg caaccttctg gagggtgagc caagggagtt tgtggagaac
     3121 tctgagtgca tacagtgcca cccagagtgc ctgcctcagg ccatgaacat cacctgcaca
     3181 ggacggggac cagacaactg tatccagtgt gcccactaca ttgacggccc ccactgcgtc
     3241 aagacctgcc cggcaggagt catgggagaa aacaacaccc tggtctggaa gtacgcagac
     3301 gccggccatg tgtgccacct gtgccatcca aactgcacct acggatgcac tgggccaggt
     3361 cttgaaggct gtccaacgaa tgggcctaag atcccgtcca tcgccactgg gatggtgggg
     3421 gccctcctct tgctgctggt ggtggccctg gggatcggcc tcttcatgcg aaggcgccac
     3481 atcgttcgga agcgcacgct gcggaggctg ctgcaggaga gggagcttgt ggagcctctt
     3541 acacccagtg gagaagctcc caaccaagct ctcttgagga tcttgaagga aactgaattc
     3601 aaaaagatca aagtgctggg ctccggtgcg ttcggcacgg tgtataaggg actctggatc
     3661 ccagaaggtg agaaagttaa aattcccgtc gctatcaagg aattaagaga agcaacatct
     3721 ccgaaagcca acaaggaaat cctcgatgaa gcctacgtga tggccagcgt ggacaacccc
     3781 cacgtgtgcc gcctgctggg catctgcctc acctccaccg tgcagctcat cacgcagctc
     3841 atgcccttcg gctgcctcct ggactatgtc cgggaacaca aagacaatat tggctcccag
     3901 tacctgctca actggtgtgt gcagatcgca aagggcatga actacttgga ggaccgtcgc
     3961 ttggtgcacc gcgacctggc agccaggaac gtactggtga aaacaccgca gcatgtcaag
     4021 atcacagatt ttgggctggc caaactgctg ggtgcggaag agaaagaata ccatgcagaa
     4081 ggaggcaaag tgcctatcaa gtggatggca ttggaatcaa ttttacacag aatctatacc
     4141 caccagagtg atgtctggag ctacggggtg accgtttggg agttgatgac ctttggatcc
     4201 aagccatatg acggaatccc tgccagcgag atctcctcca tcctggagaa aggagaacgc
     4261 ctccctcagc cacccatatg taccatcgat gtctacatga tcatggtcaa gtgctggatg
     4321 atagacgcag atagtcgccc aaagttccgt gagttgatca tcgaattctc caaaatggcc
     4381 cgagaccccc agcgctacct tgtcattcag ggggatgaaa gaatgcattt gccaagtcct
     4441 acagactcca acttctaccg tgccctgatg gatgaagaag acatggacga cgtggtggat
     4501 gccgacgagt acctcatccc acagcagggc ttcttcagca gcccctccac gtcacggact
     4561 cccctcctga gctctctgag tgcaaccagc aacaattcca ccgtggcttg cattgataga
     4621 aatgggctgc aaagctgtcc catcaaggaa gacagcttct tgcagcgata cagctcagac
     4681 cccacaggcg ccttgactga ggacagcata gacgacacct tcctcccagt gcctgaatac
     4741 ataaaccagt ccgttcccaa aaggcccgct ggctctgtgc agaatcctgt ctatcacaat
     4801 cagcctctga accccgcgcc cagcagagac ccacactacc aggaccccca cagcactgca
     4861 gtgggcaacc ccgagtatct caacactgtc cagcccacct gtgtcaacag cacattcgac
     4921 agccctgccc actgggccca gaaaggcagc caccaaatta gcctggacaa ccctgactac
     4981 cagcaggact tctttcccaa ggaagccaag ccaaatggca tctttaaggg ctccacagct
     5041 gaaaatgcag aatacctaag ggtcgcgcca caaagcagtg aatttattgg agcatagaac
     5101 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag
     5161 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     5221 accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     5281 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     5341 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     5401 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     5461 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     5521 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     5581 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     5641 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     5701 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     5761 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat
     5821 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     5881 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     5941 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     6001 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     6061 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     6121 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     6181 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     6241 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     6301 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     6361 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     6421 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     6481 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaat
     6541 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     6601 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     6661 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     6721 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     6781 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     6841 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     6901 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     6961 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7021 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7081 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7141 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     7201 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     7261 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     7321 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     7381 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     7441 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     7501 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
     7561 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     7621 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     7681 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     7741 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     7801 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     7861 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     7921 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     7981 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     8041 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     8101 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     8161 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     8221 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     8281 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     8341 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     8401 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     8461 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     8521 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     8581 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     8641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9481 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     9541 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     9601 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc
     9661 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     9721 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9781 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9841 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9901 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9961 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    10021 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    10081 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10141 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    10201 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    10261 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    10321 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    10381 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    10441 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    10501 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    10561 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    10621 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    10681 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    10741 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    10801 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    10861 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    10921 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    10981 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    11041 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    11101 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    11161 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    11221 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    11281 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
    11341 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
    11401 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
    11461 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
    11521 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
    11581 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
    11641 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
    11701 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
    11761 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
    11821 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
    11881 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
    11941 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
    12001 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
    12061 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
    12121 aagaagaagg tggagagaga gacag
//
