LOCUS       sequence-25059-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: Flag pLJM1 RagD 77L
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cgtgggcttt agcgagctgg ttngtgaacc gtcagatccg ctagcgctac cggtatggac
       61 tacaaggatg acgatgacaa gggtggtggt ggtggtgcgt cgacgatgag ccaggtgctg
      121 gggaagccgc agccgcagga cgaggacgac gcggaggagg aggaggagga ggatgagctg
      181 gtggggctag cggactacgg agacgggccc gactcctccg acgccgatcc ggacagcggc
      241 acagaggagg gagttctgga cttcagtgac cccttcagca ctgaagtgaa gccgagaatc
      301 ctgctcatgg gcctgaggag aagcggcaag tcgcttattc agaaagttgt ctttcacaaa
      361 atgtctccca acgaaactct gttcttggag agcactaata agatatgccg ggaagatgtt
      421 tccaacagct cctttgtcaa ttttcagatt tgggacttcc caggacagat tgactttttt
      481 gaccctacat ttgactatga gatgatcttc cggggaacag gagcactgat atttgtcatt
      541 gactcacagg atgattacat ggaagccctg gccaggctcc acctcacggt gaccagggcc
      601 tacaaagtga atactgacat caacttcgag gtgtttattc ataaagtgga tggtctgtca
      661 gatgaccaca aaattgaaac ccaaagagat attcaccaga gggcaaacga tgaccttgca
      721 gatgctggat tanaaaaaan tcacctcagc ttttatctga caagcatata tgatcattca
      781 atatttganc ntttancaan ttgttcanaa actgatncc
//
