LOCUS       sequence-250590-       10744 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3R1 E160*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10744)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3R1-E160*
FEATURES             Location/Qualifiers
     source          1..10744
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3458..3467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3470..3496
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5681..5705)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5758..5799
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5820..6387
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5837..6389
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5981..5998)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6208..6227
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6401..6403,6404..6406,6407..7120)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6401..6403,6404..6406,6407..7117)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6446..6467)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6707..6726)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7054..7075
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7134..7722
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7187..7207)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7605..7616)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7797..8030
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8149..8167)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8235..8339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8340..8408,8409..9200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8558..8577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9371..9959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9860..9879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10113..10130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10370..10391
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10406..10411,10412..10429,10430..10436)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10444..10460
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10449..10471
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10468..10484
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10468..10484
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10534..10553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggctacgcg tgtcgacgcc accatggttt acccatacga
     3481 tgttccagat tacgctagtg ctgaggggta ccagtacaga gcgctgtatg attataaaaa
     3541 ggaaagagaa gaagatattg acttgcactt gggtgacata ttgactgtga ataaagggtc
     3601 cttagtagct cttggattca gtgatggaca ggaagccagg cctgaagaaa ttggctggtt
     3661 aaatggctat aatgaaacca caggggaaag gggggacttt ccgggaactt atgtagaata
     3721 tattggaagg aaaaaaatct cgcctcccac accaaagccc cggccacctc ggcctcttcc
     3781 tgttgcacca ggttcttcga aaactgaagc agatgttgaa caacaagctt tgactctccc
     3841 ggatcttgca gagcagtttg cccctcctga cattgccccg cctcttctta tcaagctcgt
     3901 ggaagccatt gaaaagaaag gtctggaatg ttcaactcta tacagaacac agagctccag
     3961 caacctggca taattacgac agcttcttga ttgtgataca ccctccgtgg acttggaaat
     4021 gatcgatgtg cacgttttgg ctgacgcttt caaacgctat ctcctggact taccaaatcc
     4081 tgtcattcca gcagccgttt acagtgaaat gatttcttta gctccagaag tacaaagctc
     4141 cgaagaatat attcagctat tgaagaagct tattaggtcg cctagcatac ctcatcagta
     4201 ttggcttacg cttcagtatt tgttaaaaca tttcttcaag ctctctcaaa cctccagcaa
     4261 aaatctgttg aatgcaagag tactctctga aattttcagc cctatgcttt tcagattctc
     4321 agcagccagc tctgataata ctgaaaacct cataaaagtt atagaaattt taatctcaac
     4381 tgaatggaat gaacgacagc ctgcaccagc actgcctcct aaaccaccaa aacctactac
     4441 tgtagccaac aacggtatga ataacaatat gtccttacaa gatgctgaat ggtactgggg
     4501 agatatctcg agggaagaag tgaatgaaaa acttcgagat acagcagacg ggaccttttt
     4561 ggtacgagat gcgtctacta aaatgcatgg tgattatact cttacactaa ggaaaggggg
     4621 aaataacaaa ttaatcaaaa tatttcatcg agatgggaaa tatggcttct ctgacccatt
     4681 aaccttcagt tctgtggttg aattaataaa ccactaccgg aatgaatctc tagctcagta
     4741 taatcccaaa ttggatgtga aattacttta tccagtatcc aaataccaac aggatcaagt
     4801 tgtcaaagaa gataatattg aagctgtagg gaaaaaatta catgaatata acactcagtt
     4861 tcaagaaaaa agtcgagaat atgatagatt atatgaagaa tatacccgca catcccagga
     4921 aatccaaatg aaaaggacag ctattgaagc atttaatgaa accataaaaa tatttgaaga
     4981 acagtgccag acccaagagc ggtacagcaa agaatacata gaaaagttta aacgtgaagg
     5041 caatgagaaa gaaatacaaa ggattatgca taattatgat aagttgaagt ctcgaatcag
     5101 tgaaattatt gacagtagaa gaagattgga agaagacttg aagaagcagg cagctgagta
     5161 tcgagaaatt gacaaacgta tgaacagcat taaaccagac cttatccagc tgagaaagac
     5221 gagagaccaa tacttgatgt ggttgactca aaaaggtgtt cggcaaaaga agttgaacga
     5281 gtggttgggc aatgaaaaca ctgaagacca atattcactg gtggaagatg atgaagattt
     5341 gccccatcat gatgagaaga catggaatgt tggaagcagc aaccgaaaca aagctgaaaa
     5401 cctgttgcga gggaagcgag atggcacttt tcttgtccgg gagagcagta aacagggctg
     5461 ctatgcctgc tctgtagtgg tggacggcga agtaaagcat tgtgtcataa acaaaacagc
     5521 aactggctat ggctttgccg agccctataa cttgtacagc tctctgaaag aactggtgct
     5581 acattaccaa cacacctccc ttgtgcagca caacgactcc ctcaatgtca cactagccta
     5641 cccagtatat gcacagcaga ggcgagggcc ctgaggatcc acccagcttt cttgtacaaa
     5701 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     5761 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagat
     5821 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     5881 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     5941 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     6001 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     6061 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     6121 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     6181 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     6241 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     6301 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6361 gttttccttt gaaaaacacg atgataatat ggccacacat atggtgagca agggcgagga
     6421 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     6481 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     6541 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     6601 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     6661 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     6721 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     6781 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     6841 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     6901 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     6961 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     7021 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     7081 cgccgggatc actctcggca tggacgagct gtacaagtaa atcgatagat cctaatcaac
     7141 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7201 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7261 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7321 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7381 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7441 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7501 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7561 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7621 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7681 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     7741 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7801 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     8041 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     8101 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     8161 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     8221 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8281 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8341 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8401 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8461 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8521 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8581 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8641 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8701 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8761 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8821 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8881 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8941 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     9001 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     9061 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     9121 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9181 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9241 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9301 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9361 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9421 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9481 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9541 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9601 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9661 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9721 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9781 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9841 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9901 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9961 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10021 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10081 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10141 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
    10201 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
    10261 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
    10321 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    10381 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    10441 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
    10501 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10561 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    10621 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    10681 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10741 tagt
//
