LOCUS       sequence-250601-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR L861Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-L861Q
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    228..345
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        393..1571
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          624..1562
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1519..1539
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1591..1615
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1618..1689,1690..3552,3553..3621,3622..5250)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKQLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5252..5276)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5284..5302
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5327..5346)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5328..5346)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5352..5372)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5426..5467
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5488..6055
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5505..6057
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5649..5666)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5876..5895
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6069..6668
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6069..6088)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6565..6585
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6682..7270
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6735..6755)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7153..7164)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7345..7578
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7697..7715)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7783..7887
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7888..7956,7957..8748)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8919..9507
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9408..9427
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9661..9678
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9918..9939
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9954..9959,9960..9977,9978..9984)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9992..10008
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9997..10019
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10016..10032
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10016..10032
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10082..10101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10293..10926
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10973..11098
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11595..11828
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12013..12057
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
       61 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
      121 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
      181 atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa
      241 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
      301 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
      361 gacagcagag atccagtttg gactagtcgt gaggctccgg tgcccgtcag tgggcagagc
      421 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      481 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      541 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      601 acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct
      661 ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc
      721 ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag
      781 ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa
      841 tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt
      901 tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag
      961 atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc
     1021 agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt
     1081 agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc
     1141 cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc
     1201 ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg
     1261 agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca
     1321 cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt
     1381 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga
     1441 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt
     1501 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc
     1561 aggtgtcgtg aagcggccct gcagatatca acaagtttgt acaaaaaagc aggcaccatg
     1621 cgaccctccg ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct ctgcccggcg
     1681 agtcgggctc tggaggaaaa gaaagtttgc caaggcacga gtaacaagct cacgcagttg
     1741 ggcacttttg aagatcattt tctcagcctc cagaggatgt tcaataactg tgaggtggtc
     1801 cttgggaatt tggaaattac ctatgtgcag aggaattatg atctttcctt cttaaagacc
     1861 atccaggagg tggctggtta tgtcctcatt gccctcaaca cagtggagcg aattcctttg
     1921 gaaaacctgc agatcatcag aggaaatatg tactacgaaa attcctatgc cttagcagtc
     1981 ttatctaact atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag aaatttacag
     2041 gaaatcctgc atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc
     2101 atccagtggc gggacatagt cagcagtgac tttctcagca acatgtcgat ggacttccag
     2161 aaccacctgg gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt
     2221 gcaggagagg agaactgcca gaaactgacc aaaatcatct gtgcccagca gtgctccggg
     2281 cgctgccgtg gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc aggctgcaca
     2341 ggcccccggg agagcgactg cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag
     2401 gacacctgcc ccccactcat gctctacaac cccaccacgt accagatgga tgtgaacccc
     2461 gagggcaaat acagctttgg tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg
     2521 acagatcacg gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat ggaggaagac
     2581 ggcgtccgca agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt
     2641 attggtgaat ttaaagactc actctccata aatgctacga atattaaaca cttcaaaaac
     2701 tgcacctcca tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc
     2761 acacatactc ctcctctgga tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc
     2821 acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag
     2881 aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct tgcagtcgtc
     2941 agcctgaaca taacatcctt gggattacgc tccctcaagg agataagtga tggagatgtg
     3001 ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt
     3061 gggacctccg gtcagaaaac caaaattata agcaacagag gtgaaaacag ctgcaaggcc
     3121 acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg
     3181 gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt
     3241 ctggagggtg agccaaggga gtttgtggag aactctgagt gcatacagtg ccacccagag
     3301 tgcctgcctc aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag
     3361 tgtgcccact acattgacgg cccccactgc gtcaagacct gcccggcagg agtcatggga
     3421 gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca cctgtgccat
     3481 ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac gaatgggcct
     3541 aagatcccgt ccatcgccac tgggatggtg ggggccctcc tcttgctgct ggtggtggcc
     3601 ctggggatcg gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg
     3661 ctgctgcagg agagggagct tgtggagcct cttacaccca gtggagaagc tcccaaccaa
     3721 gctctcttga ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct gggctccggt
     3781 gcgttcggca cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc
     3841 gtcgctatca aggaattaag agaagcaaca tctccgaaag ccaacaagga aatcctcgat
     3901 gaagcctacg tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc
     3961 ctcacctcca ccgtgcagct catcacgcag ctcatgccct tcggctgcct cctggactat
     4021 gtccgggaac acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc
     4081 gcaaagggca tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg
     4141 aacgtactgg tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaacag
     4201 ctgggtgcgg aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg
     4261 gcattggaat caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg
     4321 gtgaccgttt gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc
     4381 gagatctcct ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc
     4441 gatgtctaca tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc
     4501 cgtgagttga tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt
     4561 cagggggatg aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg
     4621 atggatgaag aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag
     4681 ggcttcttca gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc
     4741 agcaacaatt ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag
     4801 gaagacagct tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc
     4861 atagacgaca ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc
     4921 gctggctctg tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga
     4981 gacccacact accaggaccc ccacagcact gcagtgggca accccgagta tctcaacact
     5041 gtccagccca cctgtgtcaa cagcacattc gacagccctg cccactgggc ccagaaaggc
     5101 agccaccaaa ttagcctgga caaccctgac taccagcagg acttctttcc caaggaagcc
     5161 aagccaaatg gcatctttaa gggctccaca gctgaaaatg cagaatacct aagggtcgcg
     5221 ccacaaagca gtgaatttat tggagcatag aacccagctt tcttgtacaa agtggtgata
     5281 tccaattaac cctcactaaa gggagtttag aaatgataaa gaattgccct atagtgagtc
     5341 gtattaccac ccaacttttc tatacaaagt ggttgatatc cagcacagtg gcggccgctc
     5401 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     5461 ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag
     5521 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     5581 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     5641 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     5701 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     5761 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     5821 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     5881 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     5941 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6001 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     6061 ccacacatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     6121 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     6181 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     6241 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     6301 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     6361 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     6421 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     6481 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6541 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6601 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6661 gtgcctaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     6721 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     6781 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     6841 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     6901 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6961 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7021 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7081 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7141 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7201 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7261 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7321 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7381 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7441 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7501 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7561 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7621 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     7681 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     7741 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7801 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7861 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7921 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7981 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8041 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8101 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8161 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8221 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8281 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8341 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8401 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8461 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8521 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8581 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8641 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8701 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8761 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8821 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8881 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8941 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9001 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9061 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9121 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9181 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9241 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9301 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9361 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9421 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9481 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9541 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9601 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9661 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     9721 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     9781 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     9841 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
     9901 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     9961 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
    10021 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
    10081 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
    10141 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
    10201 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
    10261 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
    10321 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
    10381 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
    10441 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
    10501 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
    10561 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
    10621 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
    10681 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
    10741 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    10801 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    10861 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    10921 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
    10981 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
    11041 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
    11101 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
    11161 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
    11221 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
    11281 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
    11341 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
    11401 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
    11461 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
    11521 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    11581 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    11641 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    11701 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    11761 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    11821 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    11881 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    11941 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    12001 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    12061 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    12121 gg
//
