LOCUS       sequence-250607-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR L838M.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-L838M
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    178..295
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        343..1521
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          574..1512
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1469..1489
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1541..1565
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1568..1639,1640..3502,3503..3571,3572..5200)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDMAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5202..5226)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5234..5252
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5277..5296)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5278..5296)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5302..5322)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5376..5417
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5438..6005
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5455..6007
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5599..5616)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5826..5845
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6019..6618
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6019..6038)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6515..6535
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6632..7220
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6685..6705)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7103..7114)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7295..7528
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7647..7665)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7733..7837
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7838..7906,7907..8698)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8869..9457
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9358..9377
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9611..9628
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9868..9889
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9904..9909,9910..9927,9928..9934)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9942..9958
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9947..9969
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9966..9982
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9966..9982
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10032..10051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10243..10876
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10923..11048
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11545..11778
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11963..12007
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
       61 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
      121 tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg gcagtattca tccacaattt
      181 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
      241 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
      301 ttattacagg gacagcagag atccagtttg gactagtcgt gaggctccgg tgcccgtcag
      361 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
      421 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
      481 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
      541 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
      601 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
      661 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
      721 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
      781 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
      841 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
      901 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
      961 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     1021 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     1081 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     1141 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     1201 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     1261 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     1321 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     1381 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     1441 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     1501 cttccatttc aggtgtcgtg aagcggccct gcagatatca acaagtttgt acaaaaaagc
     1561 aggcaccatg cgaccctccg ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct
     1621 ctgcccggcg agtcgggctc tggaggaaaa gaaagtttgc caaggcacga gtaacaagct
     1681 cacgcagttg ggcacttttg aagatcattt tctcagcctc cagaggatgt tcaataactg
     1741 tgaggtggtc cttgggaatt tggaaattac ctatgtgcag aggaattatg atctttcctt
     1801 cttaaagacc atccaggagg tggctggtta tgtcctcatt gccctcaaca cagtggagcg
     1861 aattcctttg gaaaacctgc agatcatcag aggaaatatg tactacgaaa attcctatgc
     1921 cttagcagtc ttatctaact atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag
     1981 aaatttacag gaaatcctgc atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa
     2041 cgtggagagc atccagtggc gggacatagt cagcagtgac tttctcagca acatgtcgat
     2101 ggacttccag aaccacctgg gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag
     2161 ctgctggggt gcaggagagg agaactgcca gaaactgacc aaaatcatct gtgcccagca
     2221 gtgctccggg cgctgccgtg gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc
     2281 aggctgcaca ggcccccggg agagcgactg cctggtctgc cgcaaattcc gagacgaagc
     2341 cacgtgcaag gacacctgcc ccccactcat gctctacaac cccaccacgt accagatgga
     2401 tgtgaacccc gagggcaaat acagctttgg tgccacctgc gtgaagaagt gtccccgtaa
     2461 ttatgtggtg acagatcacg gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat
     2521 ggaggaagac ggcgtccgca agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa
     2581 cggaataggt attggtgaat ttaaagactc actctccata aatgctacga atattaaaca
     2641 cttcaaaaac tgcacctcca tcagtggcga tctccacatc ctgccggtgg catttagggg
     2701 tgactccttc acacatactc ctcctctgga tccacaggaa ctggatattc tgaaaaccgt
     2761 aaaggaaatc acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca
     2821 tgcctttgag aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct
     2881 tgcagtcgtc agcctgaaca taacatcctt gggattacgc tccctcaagg agataagtga
     2941 tggagatgtg ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa
     3001 aaaactgttt gggacctccg gtcagaaaac caaaattata agcaacagag gtgaaaacag
     3061 ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc
     3121 ggagcccagg gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa
     3181 gtgcaacctt ctggagggtg agccaaggga gtttgtggag aactctgagt gcatacagtg
     3241 ccacccagag tgcctgcctc aggccatgaa catcacctgc acaggacggg gaccagacaa
     3301 ctgtatccag tgtgcccact acattgacgg cccccactgc gtcaagacct gcccggcagg
     3361 agtcatggga gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca
     3421 cctgtgccat ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac
     3481 gaatgggcct aagatcccgt ccatcgccac tgggatggtg ggggccctcc tcttgctgct
     3541 ggtggtggcc ctggggatcg gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac
     3601 gctgcggagg ctgctgcagg agagggagct tgtggagcct cttacaccca gtggagaagc
     3661 tcccaaccaa gctctcttga ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct
     3721 gggctccggt gcgttcggca cggtgtataa gggactctgg atcccagaag gtgagaaagt
     3781 taaaattccc gtcgctatca aggaattaag agaagcaaca tctccgaaag ccaacaagga
     3841 aatcctcgat gaagcctacg tgatggccag cgtggacaac ccccacgtgt gccgcctgct
     3901 gggcatctgc ctcacctcca ccgtgcagct catcacgcag ctcatgccct tcggctgcct
     3961 cctggactat gtccgggaac acaaagacaa tattggctcc cagtacctgc tcaactggtg
     4021 tgtgcagatc gcaaagggca tgaactactt ggaggaccgt cgcttggtgc accgcgacat
     4081 ggcagccagg aacgtactgg tgaaaacacc gcagcatgtc aagatcacag attttgggct
     4141 ggccaaactg ctgggtgcgg aagagaaaga ataccatgca gaaggaggca aagtgcctat
     4201 caagtggatg gcattggaat caattttaca cagaatctat acccaccaga gtgatgtctg
     4261 gagctacggg gtgaccgttt gggagttgat gacctttgga tccaagccat atgacggaat
     4321 ccctgccagc gagatctcct ccatcctgga gaaaggagaa cgcctccctc agccacccat
     4381 atgtaccatc gatgtctaca tgatcatggt caagtgctgg atgatagacg cagatagtcg
     4441 cccaaagttc cgtgagttga tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta
     4501 ccttgtcatt cagggggatg aaagaatgca tttgccaagt cctacagact ccaacttcta
     4561 ccgtgccctg atggatgaag aagacatgga cgacgtggtg gatgccgacg agtacctcat
     4621 cccacagcag ggcttcttca gcagcccctc cacgtcacgg actcccctcc tgagctctct
     4681 gagtgcaacc agcaacaatt ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg
     4741 tcccatcaag gaagacagct tcttgcagcg atacagctca gaccccacag gcgccttgac
     4801 tgaggacagc atagacgaca ccttcctccc agtgcctgaa tacataaacc agtccgttcc
     4861 caaaaggccc gctggctctg tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc
     4921 gcccagcaga gacccacact accaggaccc ccacagcact gcagtgggca accccgagta
     4981 tctcaacact gtccagccca cctgtgtcaa cagcacattc gacagccctg cccactgggc
     5041 ccagaaaggc agccaccaaa ttagcctgga caaccctgac taccagcagg acttctttcc
     5101 caaggaagcc aagccaaatg gcatctttaa gggctccaca gctgaaaatg cagaatacct
     5161 aagggtcgcg ccacaaagca gtgaatttat tggagcatag aacccagctt tcttgtacaa
     5221 agtggtgata tccaattaac cctcactaaa gggtgattga atagtagatt gtttaaccct
     5281 atagtgagtc gtattaccac ccaacttttc tatacaaagt ggttgatatc cagcacagtg
     5341 gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc
     5401 tcggtctcga ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta
     5461 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     5521 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     5581 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     5641 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     5701 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     5761 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     5821 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     5881 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     5941 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     6001 gataatatgg ccacacatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6061 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6121 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6181 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6241 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6301 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6361 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6421 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6481 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6541 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6601 cgcaagcccg gtgcctaaat cgatagatcc taatcaacct ctggattaca aaatttgtga
     6661 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6721 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6781 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6841 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6901 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6961 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     7021 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     7081 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7141 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7201 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
     7261 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7321 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7381 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7441 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7501 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     7561 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
     7621 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     7681 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     7741 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     7801 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     7861 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     7921 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     7981 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8041 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     8101 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8161 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8221 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8281 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8341 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8401 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8461 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8521 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     8581 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     8641 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     8701 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     8761 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     8821 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     8881 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     8941 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9001 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9061 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9121 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     9181 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     9241 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     9301 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     9361 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     9421 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     9481 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     9541 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     9601 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     9661 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
     9721 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9781 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
     9841 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9901 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9961 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
    10021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    10081 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    10141 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    10201 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
    10261 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
    10321 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
    10381 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
    10441 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
    10501 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
    10561 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
    10621 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
    10681 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    10741 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    10801 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    10861 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
    10921 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
    10981 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
    11041 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
    11101 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
    11161 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
    11221 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
    11281 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
    11341 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
    11401 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    11461 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    11521 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    11581 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    11641 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    11701 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    11761 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    11821 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    11881 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    11941 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    12001 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    12061 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    12121 ag
//
