LOCUS       sequence-250610-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR L718P.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-L718P
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    103..220
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        268..1446
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          499..1437
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1394..1414
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1466..1490
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1493..1564,1565..3427,3428..3496,3497..5125)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVPGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5127..5151)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5159..5177
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5202..5221)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5203..5221)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5227..5247)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5301..5342
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5363..5930
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5380..5932
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5524..5541)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5751..5770
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5944..6543
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5944..5963)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6440..6460
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6557..7145
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6610..6630)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7028..7039)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7220..7453
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7572..7590)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7658..7762
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7763..7831,7832..8623)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8794..9382
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9283..9302
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9536..9553
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9793..9814
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9829..9834,9835..9852,9853..9859)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9867..9883
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9872..9894
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9891..9907
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9891..9907
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9957..9976
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10168..10801
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10848..10973
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11470..11703
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11888..11932
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
       61 gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg
      121 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
      181 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
      241 cagagatcca gtttggacta gtcgtgaggc tccggtgccc gtcagtgggc agagcgcaca
      301 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
      361 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
      421 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
      481 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
      541 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
      601 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
      661 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
      721 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
      781 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
      841 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
      901 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
      961 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     1021 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     1081 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     1141 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     1201 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     1261 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     1321 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     1381 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     1441 tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa aaagcaggca ccatgcgacc
     1501 ctccgggacg gccggggcag cgctcctggc gctgctggct gcgctctgcc cggcgagtcg
     1561 ggctctggag gaaaagaaag tttgccaagg cacgagtaac aagctcacgc agttgggcac
     1621 ttttgaagat cattttctca gcctccagag gatgttcaat aactgtgagg tggtccttgg
     1681 gaatttggaa attacctatg tgcagaggaa ttatgatctt tccttcttaa agaccatcca
     1741 ggaggtggct ggttatgtcc tcattgccct caacacagtg gagcgaattc ctttggaaaa
     1801 cctgcagatc atcagaggaa atatgtacta cgaaaattcc tatgccttag cagtcttatc
     1861 taactatgat gcaaataaaa ccggactgaa ggagctgccc atgagaaatt tacaggaaat
     1921 cctgcatggc gccgtgcggt tcagcaacaa ccctgccctg tgcaacgtgg agagcatcca
     1981 gtggcgggac atagtcagca gtgactttct cagcaacatg tcgatggact tccagaacca
     2041 cctgggcagc tgccaaaagt gtgatccaag ctgtcccaat gggagctgct ggggtgcagg
     2101 agaggagaac tgccagaaac tgaccaaaat catctgtgcc cagcagtgct ccgggcgctg
     2161 ccgtggcaag tcccccagtg actgctgcca caaccagtgt gctgcaggct gcacaggccc
     2221 ccgggagagc gactgcctgg tctgccgcaa attccgagac gaagccacgt gcaaggacac
     2281 ctgcccccca ctcatgctct acaaccccac cacgtaccag atggatgtga accccgaggg
     2341 caaatacagc tttggtgcca cctgcgtgaa gaagtgtccc cgtaattatg tggtgacaga
     2401 tcacggctcg tgcgtccgag cctgtggggc cgacagctat gagatggagg aagacggcgt
     2461 ccgcaagtgt aagaagtgcg aagggccttg ccgcaaagtg tgtaacggaa taggtattgg
     2521 tgaatttaaa gactcactct ccataaatgc tacgaatatt aaacacttca aaaactgcac
     2581 ctccatcagt ggcgatctcc acatcctgcc ggtggcattt aggggtgact ccttcacaca
     2641 tactcctcct ctggatccac aggaactgga tattctgaaa accgtaaagg aaatcacagg
     2701 gtttttgctg attcaggctt ggcctgaaaa caggacggac ctccatgcct ttgagaacct
     2761 agaaatcata cgcggcagga ccaagcaaca tggtcagttt tctcttgcag tcgtcagcct
     2821 gaacataaca tccttgggat tacgctccct caaggagata agtgatggag atgtgataat
     2881 ttcaggaaac aaaaatttgt gctatgcaaa tacaataaac tggaaaaaac tgtttgggac
     2941 ctccggtcag aaaaccaaaa ttataagcaa cagaggtgaa aacagctgca aggccacagg
     3001 ccaggtctgc catgccttgt gctcccccga gggctgctgg ggcccggagc ccagggactg
     3061 cgtctcttgc cggaatgtca gccgaggcag ggaatgcgtg gacaagtgca accttctgga
     3121 gggtgagcca agggagtttg tggagaactc tgagtgcata cagtgccacc cagagtgcct
     3181 gcctcaggcc atgaacatca cctgcacagg acggggacca gacaactgta tccagtgtgc
     3241 ccactacatt gacggccccc actgcgtcaa gacctgcccg gcaggagtca tgggagaaaa
     3301 caacaccctg gtctggaagt acgcagacgc cggccatgtg tgccacctgt gccatccaaa
     3361 ctgcacctac ggatgcactg ggccaggtct tgaaggctgt ccaacgaatg ggcctaagat
     3421 cccgtccatc gccactggga tggtgggggc cctcctcttg ctgctggtgg tggccctggg
     3481 gatcggcctc ttcatgcgaa ggcgccacat cgttcggaag cgcacgctgc ggaggctgct
     3541 gcaggagagg gagcttgtgg agcctcttac acccagtgga gaagctccca accaagctct
     3601 cttgaggatc ttgaaggaaa ctgaattcaa aaagatcaaa gtgccgggct ccggtgcgtt
     3661 cggcacggtg tataagggac tctggatccc agaaggtgag aaagttaaaa ttcccgtcgc
     3721 tatcaaggaa ttaagagaag caacatctcc gaaagccaac aaggaaatcc tcgatgaagc
     3781 ctacgtgatg gccagcgtgg acaaccccca cgtgtgccgc ctgctgggca tctgcctcac
     3841 ctccaccgtg cagctcatca cgcagctcat gcccttcggc tgcctcctgg actatgtccg
     3901 ggaacacaaa gacaatattg gctcccagta cctgctcaac tggtgtgtgc agatcgcaaa
     3961 gggcatgaac tacttggagg accgtcgctt ggtgcaccgc gacctggcag ccaggaacgt
     4021 actggtgaaa acaccgcagc atgtcaagat cacagatttt gggctggcca aactgctggg
     4081 tgcggaagag aaagaatacc atgcagaagg aggcaaagtg cctatcaagt ggatggcatt
     4141 ggaatcaatt ttacacagaa tctataccca ccagagtgat gtctggagct acggggtgac
     4201 cgtttgggag ttgatgacct ttggatccaa gccatatgac ggaatccctg ccagcgagat
     4261 ctcctccatc ctggagaaag gagaacgcct ccctcagcca cccatatgta ccatcgatgt
     4321 ctacatgatc atggtcaagt gctggatgat agacgcagat agtcgcccaa agttccgtga
     4381 gttgatcatc gaattctcca aaatggcccg agacccccag cgctaccttg tcattcaggg
     4441 ggatgaaaga atgcatttgc caagtcctac agactccaac ttctaccgtg ccctgatgga
     4501 tgaagaagac atggacgacg tggtggatgc cgacgagtac ctcatcccac agcagggctt
     4561 cttcagcagc ccctccacgt cacggactcc cctcctgagc tctctgagtg caaccagcaa
     4621 caattccacc gtggcttgca ttgatagaaa tgggctgcaa agctgtccca tcaaggaaga
     4681 cagcttcttg cagcgataca gctcagaccc cacaggcgcc ttgactgagg acagcataga
     4741 cgacaccttc ctcccagtgc ctgaatacat aaaccagtcc gttcccaaaa ggcccgctgg
     4801 ctctgtgcag aatcctgtct atcacaatca gcctctgaac cccgcgccca gcagagaccc
     4861 acactaccag gacccccaca gcactgcagt gggcaacccc gagtatctca acactgtcca
     4921 gcccacctgt gtcaacagca cattcgacag ccctgcccac tgggcccaga aaggcagcca
     4981 ccaaattagc ctggacaacc ctgactacca gcaggacttc tttcccaagg aagccaagcc
     5041 aaatggcatc tttaagggct ccacagctga aaatgcagaa tacctaaggg tcgcgccaca
     5101 aagcagtgaa tttattggag catagaaccc agctttcttg tacaaagtgg tgatatccaa
     5161 ttaaccctca ctaaaggtat tagagagaaa ttgtagagat tccctatagt gagtcgtatt
     5221 accacccaac ttttctatac aaagtggttg atatccagca cagtggcggc cgctcgagtc
     5281 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     5341 cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc cgaagccgct
     5401 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5461 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5521 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5581 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5641 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5701 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5761 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     5821 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     5881 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     5941 catatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6001 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6061 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6121 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6181 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6241 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6301 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6361 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6421 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6481 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6541 taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     6601 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     6661 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     6721 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     6781 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     6841 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     6901 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     6961 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7021 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7081 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7141 cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7201 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7261 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7321 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7381 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7441 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     7501 aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg
     7561 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     7621 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7981 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     8041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     8461 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     8521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8941 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9181 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9361 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9421 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     9481 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     9541 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     9601 gccgattcat taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc
     9661 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9721 ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga
     9781 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9841 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9901 ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9961 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10021 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10081 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10141 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
    10201 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
    10261 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
    10321 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
    10381 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
    10441 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
    10501 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
    10561 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
    10621 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
    10681 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
    10741 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
    10801 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
    10861 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
    10921 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
    10981 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
    11041 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
    11101 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
    11161 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
    11221 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
    11281 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
    11341 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    11401 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    11461 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    11521 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    11581 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    11641 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    11701 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    11761 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    11821 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    11881 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    11941 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    12001 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    12061 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
    12121 aa
//
