LOCUS       sequence-250625-       10712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3R1 F398Lfs*6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10712)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3R1-F398Lfs*6
FEATURES             Location/Qualifiers
     source          1..10712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..45
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(219..221,222..224,225..938)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(219..221,222..224,225..935)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(264..285)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(525..544)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     872..893
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    952..1540
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1005..1025)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1423..1434)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1615..1848
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1967..1985)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2053..2157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2158..2226,2227..3018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2376..2395)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3189..3777
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3678..3697
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3931..3948
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4188..4209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4224..4229,4230..4247,4248..4254)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4262..4278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4267..4289
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4286..4302
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4286..4302
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4352..4371
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4563..5196
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5243..5368
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5865..6098
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6283..6327
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6620..6737
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6785..7963
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7016..7954
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7911..7931
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7983..8007
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8013..8039
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(10211..10235)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10288..10329
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(10350..10712,1..205)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(10367..10712,1..207)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10511..10528)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
       61 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
      121 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
      181 tttcctttga aaaacacgat gataatatgg ccacacatat ggtgagcaag ggcgaggagc
      241 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      301 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      361 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      421 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      481 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      541 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      601 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      661 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      721 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      781 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      841 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      901 ccgggatcac tctcggcatg gacgagctgt acaagtaaat cgatagatcc taatcaacct
      961 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     1021 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     1081 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     1141 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     1201 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     1261 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     1321 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     1381 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     1441 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     1501 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     1561 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     1621 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     1681 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     1741 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1801 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     1861 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     1921 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     1981 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2161 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2221 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2281 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2341 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2401 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2461 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2521 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2581 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2641 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2701 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2761 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2821 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2881 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2941 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3001 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3061 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3301 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3541 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3601 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3661 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3721 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3781 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3841 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3901 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3961 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
     4021 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4081 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
     4141 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4201 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4261 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
     4321 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4381 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     4441 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     4501 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     4561 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     4621 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     4681 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     4741 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     4801 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     4861 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     4921 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     4981 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     5041 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5101 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5161 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     5221 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     5281 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     5341 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     5401 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     5461 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     5521 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     5581 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     5641 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     5701 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     5761 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     5821 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     5881 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     5941 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     6001 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     6061 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     6121 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     6181 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     6241 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     6301 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     6361 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     6421 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     6481 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     6541 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa
     6601 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     6661 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     6721 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggactagtc
     6781 gtgaggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     6841 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     6901 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     6961 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     7021 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     7081 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     7141 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     7201 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     7261 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     7321 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     7381 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     7441 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     7501 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     7561 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     7621 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     7681 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     7741 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     7801 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     7861 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     7921 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaagcggcc ctgcagatat
     7981 caacaagttt gtacaaaaaa gttggcatgg tttacccata cgatgttcca gattacgcta
     8041 gtgctgaggg gtaccagtac agagcgctgt atgattataa aaaggaaaga gaagaagata
     8101 ttgacttgca cttgggtgac atattgactg tgaataaagg gtccttagta gctcttggat
     8161 tcagtgatgg acaggaagcc aggcctgaag aaattggctg gttaaatggc tataatgaaa
     8221 ccacagggga aaggggggac tttccgggaa cttatgtaga atatattgga aggaaaaaaa
     8281 tctcgcctcc cacaccaaag ccccggccac ctcggcctct tcctgttgca ccaggttctt
     8341 cgaaaactga agcagatgtt gaacaacaag ctttgactct cccggatctt gcagagcagt
     8401 ttgcccctcc tgacattgcc ccgcctcttc ttatcaagct cgtggaagcc attgaaaaga
     8461 aaggtctgga atgttcaact ctatacagaa cacagagctc cagcaacctg gcagaattac
     8521 gacagcttct tgattgtgat acaccctccg tggacttgga aatgatcgat gtgcacgttt
     8581 tggctgacgc tttcaaacgc tatctcctgg acttaccaaa tcctgtcatt ccagcagccg
     8641 tttacagtga aatgatttct ttagctccag aagtacaaag ctccgaagaa tatattcagc
     8701 tattgaagaa gcttattagg tcgcctagca tacctcatca gtattggctt acgcttcagt
     8761 atttgttaaa acatttcttc aagctctctc aaacctccag caaaaatctg ttgaatgcaa
     8821 gagtactctc tgaaattttc agccctatgc ttttcagatt ctcagcagcc agctctgata
     8881 atactgaaaa cctcataaaa gttatagaaa ttttaatctc aactgaatgg aatgaacgac
     8941 agcctgcacc agcactgcct cctaaaccac caaaacctac tactgtagcc aacaacggta
     9001 tgaataacaa tatgtcctta caagatgctg aatggtactg gggagatatc tcgagggaag
     9061 aagtgaatga aaaacttcga gatacagcag acgggacctt tttggtacga gatgcgtcta
     9121 ctaaaatgca tggtgattat actcttacac taaggaaagg gggaaataac aaattaatca
     9181 aaatatttca tcgagatggg aaatatggct tctctgaccc attaaccctt cagttctgtg
     9241 gttgaattaa taaaccacta ccggaatgaa tctctagctc agtataatcc caaattggat
     9301 gtgaaattac tttatccagt atccaaatac caacaggatc aagttgtcaa agaagataat
     9361 attgaagctg tagggaaaaa attacatgaa tataacactc agtttcaaga aaaaagtcga
     9421 gaatatgata gattatatga agaatatacc cgcacatccc aggaaatcca aatgaaaagg
     9481 acagctattg aagcatttaa tgaaaccata aaaatatttg aagaacagtg ccagacccaa
     9541 gagcggtaca gcaaagaata catagaaaag tttaaacgtg aaggcaatga gaaagaaata
     9601 caaaggatta tgcataatta tgataagttg aagtctcgaa tcagtgaaat tattgacagt
     9661 agaagaagat tggaagaaga cttgaagaag caggcagctg agtatcgaga aattgacaaa
     9721 cgtatgaaca gcattaaacc agaccttatc cagctgagaa agacgagaga ccaatacttg
     9781 atgtggttga ctcaaaaagg tgttcggcaa aagaagttga acgagtggtt gggcaatgaa
     9841 aacactgaag accaatattc actggtggaa gatgatgaag atttgcccca tcatgatgag
     9901 aagacatgga atgttggaag cagcaaccga aacaaagctg aaaacctgtt gcgagggaag
     9961 cgagatggca cttttcttgt ccgggagagc agtaaacagg gctgctatgc ctgctctgta
    10021 gtggtggacg gcgaagtaaa gcattgtgtc ataaacaaaa cagcaactgg ctatggcttt
    10081 gccgagccct ataacttgta cagctctctg aaagaactgg tgctacatta ccaacacacc
    10141 tcccttgtgc agcacaacga ctccctcaat gtcacactag cctacccagt atatgcacag
    10201 cagaggcgat gcccaacttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
    10261 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
    10321 gattctacgc gtaccggtta gtaatgagat ccctcccccc cccctaacgt tactggccga
    10381 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
    10441 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
    10501 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
    10561 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
    10621 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
    10681 aaggcggcac aaccccagtg ccacgttgtg ag
//
