LOCUS       sequence-25063-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Ornitz DM, Moreadith RW, Leder P
  TITLE     Binary system for regulating transgene expression in mice: targeting
            int-2 gene expression with yeast GAL4/UAS control elements.
  JOURNAL   Proc Natl Acad Sci U S A 1991 Feb 1;88(3):698-702.
  PUBMED    1846961
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1991-02-1"; volume: "88(3)"; pages: 
            "698-702"
COMMENT     Sequence Label: MMTV-GAL4-SV40
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..31
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15..31
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    28..162
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(52..71)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     106..125
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(434..454)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          584..649
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gcggnggcgg ccgctctaga actagtggat ccagacatga taagatacat tgatgagttt
       61 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
      121 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
      181 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
      241 tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc aaatattttt
      301 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag
      361 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt
      421 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag
      481 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg
      541 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa
      601 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt
      661 aaatctctgt aggtagtttg tccaattatg tcacacnnnn nanananntn cnnnnnaaan
      721 ntccntnnaa tcccnncccc ncnacctgca ngaantcgat atcagctntg atanccctcg
      781 tanatatttt catgagantt aggtccnaaa aaaa
//
