LOCUS       sequence-250635-       10711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3R1 G353R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3R1-G353R
FEATURES             Location/Qualifiers
     source          1..10711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     171..190
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(364..366,367..369,370..1083)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(364..366,367..369,370..1080)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(409..430)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(670..689)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1017..1038
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1097..1685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1150..1170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1568..1579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1760..1993
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(2112..2130)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2198..2302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2303..2371,2372..3163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2521..2540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3334..3922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3823..3842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4076..4093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4333..4354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4369..4374,4375..4392,4393..4399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4407..4423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4412..4434
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4431..4447
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4431..4447
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4497..4516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4708..5341
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5388..5513
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6010..6243
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6428..6472
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6765..6882
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6930..8108
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7161..8099
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8056..8076
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    8128..8152
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8158..8184
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(10355..10379)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10432..10473
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(10494..10711,1..350)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    join(10511..10711,1..352)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10655..10672)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
       61 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
      121 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
      181 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
      241 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
      301 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
      361 catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      541 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      601 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
      661 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      721 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      781 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
      841 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
      901 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
      961 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     1021 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1081 taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     1141 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     1201 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     1261 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     1321 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     1381 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     1441 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     1501 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     1561 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     1621 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     1681 cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     1741 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     1801 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     1861 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     1921 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     1981 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     2041 aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg
     2101 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     2161 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2221 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2281 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     2341 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2401 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2461 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2521 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     2581 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2641 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2701 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2761 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2821 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2881 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2941 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3001 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3061 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3121 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3181 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3241 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     3301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3481 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3961 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4021 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4081 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     4141 gccgattcat taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc
     4201 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     4261 ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga
     4321 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     4381 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     4441 ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4501 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     4561 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
     4621 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
     4681 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag
     4741 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     4801 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     4861 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     4921 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     4981 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     5041 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     5101 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     5161 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     5221 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     5281 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     5341 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     5401 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     5461 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     5521 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     5581 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     5641 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     5701 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     5761 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     5821 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     5881 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     5941 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     6001 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     6061 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     6121 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     6181 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     6241 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     6301 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     6361 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     6421 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     6481 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     6541 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     6601 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     6661 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     6721 tcgacggtat cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg
     6781 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     6841 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     6901 agcagagatc cagtttggac tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     6961 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     7021 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     7081 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     7141 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     7201 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     7261 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     7321 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     7381 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     7441 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     7501 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     7561 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     7621 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     7681 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     7741 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     7801 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     7861 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     7921 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     7981 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     8041 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     8101 tgtcgtgaag cggccctgca gatatcaaca agtttgtaca aaaaagttgg catggtttac
     8161 ccatacgatg ttccagatta cgctagtgct gaggggtacc agtacagagc gctgtatgat
     8221 tataaaaagg aaagagaaga agatattgac ttgcacttgg gtgacatatt gactgtgaat
     8281 aaagggtcct tagtagctct tggattcagt gatggacagg aagccaggcc tgaagaaatt
     8341 ggctggttaa atggctataa tgaaaccaca ggggaaaggg gggactttcc gggaacttat
     8401 gtagaatata ttggaaggaa aaaaatctcg cctcccacac caaagccccg gccacctcgg
     8461 cctcttcctg ttgcaccagg ttcttcgaaa actgaagcag atgttgaaca acaagctttg
     8521 actctcccgg atcttgcaga gcagtttgcc cctcctgaca ttgccccgcc tcttcttatc
     8581 aagctcgtgg aagccattga aaagaaaggt ctggaatgtt caactctata cagaacacag
     8641 agctccagca acctggcaga attacgacag cttcttgatt gtgatacacc ctccgtggac
     8701 ttggaaatga tcgatgtgca cgttttggct gacgctttca aacgctatct cctggactta
     8761 ccaaatcctg tcattccagc agccgtttac agtgaaatga tttctttagc tccagaagta
     8821 caaagctccg aagaatatat tcagctattg aagaagctta ttaggtcgcc tagcatacct
     8881 catcagtatt ggcttacgct tcagtatttg ttaaaacatt tcttcaagct ctctcaaacc
     8941 tccagcaaaa atctgttgaa tgcaagagta ctctctgaaa ttttcagccc tatgcttttc
     9001 agattctcag cagccagctc tgataatact gaaaacctca taaaagttat agaaatttta
     9061 atctcaactg aatggaatga acgacagcct gcaccagcac tgcctcctaa accaccaaaa
     9121 cctactactg tagccaacaa cggtatgaat aacaatatgt ccttacaaga tgctgaatgg
     9181 tactggggag atatctcgag ggaagaagtg aatgaaaaac ttcgagatac agcagacagg
     9241 acctttttgg tacgagatgc gtctactaaa atgcatggtg attatactct tacactaagg
     9301 aaagggggaa ataacaaatt aatcaaaata tttcatcgag atgggaaata tggcttctct
     9361 gacccattaa ccttcagttc tgtggttgaa ttaataaacc actaccggaa tgaatctcta
     9421 gctcagtata atcccaaatt ggatgtgaaa ttactttatc cagtatccaa ataccaacag
     9481 gatcaagttg tcaaagaaga taatattgaa gctgtaggga aaaaattaca tgaatataac
     9541 actcagtttc aagaaaaaag tcgagaatat gatagattat atgaagaata tacccgcaca
     9601 tcccaggaaa tccaaatgaa aaggacagct attgaagcat ttaatgaaac cataaaaata
     9661 tttgaagaac agtgccagac ccaagagcgg tacagcaaag aatacataga aaagtttaaa
     9721 cgtgaaggca atgagaaaga aatacaaagg attatgcata attatgataa gttgaagtct
     9781 cgaatcagtg aaattattga cagtagaaga agattggaag aagacttgaa gaagcaggca
     9841 gctgagtatc gagaaattga caaacgtatg aacagcatta aaccagacct tatccagctg
     9901 agaaagacga gagaccaata cttgatgtgg ttgactcaaa aaggtgttcg gcaaaagaag
     9961 ttgaacgagt ggttgggcaa tgaaaacact gaagaccaat attcactggt ggaagatgat
    10021 gaagatttgc cccatcatga tgagaagaca tggaatgttg gaagcagcaa ccgaaacaaa
    10081 gctgaaaacc tgttgcgagg gaagcgagat ggcacttttc ttgtccggga gagcagtaaa
    10141 cagggctgct atgcctgctc tgtagtggtg gacggcgaag taaagcattg tgtcataaac
    10201 aaaacagcaa ctggctatgg ctttgccgag ccctataact tgtacagctc tctgaaagaa
    10261 ctggtgctac attaccaaca cacctccctt gtgcagcaca acgactccct caatgtcaca
    10321 ctagcctacc cagtatatgc acagcagagg cgatgcccaa ctttcttgta caaagtggtt
    10381 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
    10441 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agatccctcc
    10501 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
    10561 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
    10621 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
    10681 ttgaatgtcg tgaaggaagc agttcctctg g
//
