LOCUS       sequence-250636-       10910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF S467L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-S467L
FEATURES             Location/Qualifiers
     source          1..10910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      45..54
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2349..2373)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2381..2399
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2424..2443)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2425..2443)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2449..2469)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             2523..2564
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2585..3152
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2602..3154
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2746..2763)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2973..2992
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3166..3168,3169..3171,3172..3885)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3166..3168,3169..3171,3172..3882)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3211..3232)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3472..3491)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3819..3840
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3899..4487
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3952..3972)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4370..4381)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4562..4795
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4914..4932)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5000..5104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5105..5173,5174..5965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5323..5342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6136..6724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6625..6644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6878..6895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7135..7156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7171..7176,7177..7194,7195..7201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7209..7225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7214..7236
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7233..7249
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7233..7249
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7299..7318
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7510..8143
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8190..8315
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8812..9045
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9230..9274
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9567..9684
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9732..10910
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9963..10901
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     10858..10878
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 agcggccctg cagatatcaa caagtttgta caaaaaagca ggcaccatgg cggcgctgag
       61 cggtggcggt ggtggcggcg cggagccggg ccaggctctg ttcaacgggg acatggagcc
      121 cgaggccggc gccggcgccg gcgccgcggc ctcttcggct gcggaccctg ccattccgga
      181 ggaggtgtgg aatatcaaac aaatgattaa gttgacacag gaacatatag aggccctatt
      241 ggacaaattt ggtggggagc ataatccacc atcaatatat ctggaggcct atgaagaata
      301 caccagcaag ctagatgcac tccaacaaag agaacaacag ttattggaat ctctggggaa
      361 cggaactgat ttttctgttt ctagctctgc atcaatggat accgttacat cttcttcctc
      421 ttctagcctt tcagtgctac cttcatctct ttcagttttt caaaatccca cagatgtggc
      481 acggagcaac cccaagtcac cacaaaaacc tatcgttaga gtcttcctgc ccaacaaaca
      541 gaggacagtg gtacctgcaa ggtgtggagt tacagtccga gacagtctaa agaaagcact
      601 gatgatgaga ggtctaatcc cagagtgctg tgctgtttac agaattcagg atggagagaa
      661 gaaaccaatt ggttgggaca ctgatatttc ctggcttact ggagaagaat tgcatgtgga
      721 agtgttggag aatgttccac ttacaacaca caactttgta cgaaaaacgt ttttcacctt
      781 agcattttgt gacttttgtc gaaagctgct tttccagggt ttccgctgtc aaacatgtgg
      841 ttataaattt caccagcgtt gtagtacaga agttccactg atgtgtgtta attatgacca
      901 acttgatttg ctgtttgtct ccaagttctt tgaacaccac ccaataccac aggaagaggc
      961 gtccttagca gagactgccc taacatctgg atcatcccct tccgcacccg cctcggactc
     1021 tattgggccc caaattctca ccagtccgtc tccttcaaaa tccattccaa ttccacagcc
     1081 cttccgacca gcagatgaag atcatcgaaa tcaatttggg caacgagacc gatcctcatc
     1141 agctcccaat gtgcatataa acacaataga acctgtcaat attgatgact tgattagaga
     1201 ccaaggattt cgtggtgatg gaggatcaac cacaggtttg tctgctaccc cccctgcctc
     1261 attacctggc tcactaacta acgtgaaagc cttacagaaa tctccaggac ctcagcgaga
     1321 aaggaagtca tcttcatcct cagaagacag gaatcgaatg aaaacacttg gtagacggga
     1381 ctcgagtgat gattgggaga ttcctgatgg gcagattaca gtgggacaaa gaattggatc
     1441 tggattattt ggaacagtct acaagggaaa gtggcatggt gatgtggcag tgaaaatgtt
     1501 gaatgtgaca gcacctacac ctcagcagtt acaagccttc aaaaatgaag taggagtact
     1561 caggaaaaca cgacatgtga atatcctact cttcatgggc tattccacaa agccacaact
     1621 ggctattgtt acccagtggt gtgagggctc cagcttgtat caccatctcc atatcattga
     1681 gaccaaattt gagatgatca aacttataga tattgcacga cagactgcac agggcatgga
     1741 ttacttacac gccaagtcaa tcatccacag agacctcaag agtaataata tatttcttca
     1801 tgaagacctc acagtaaaaa taggtgattt tggtctagct acagtgaaat ctcgatggag
     1861 tgggtcccat cagtttgaac agttgtctgg atccattttg tggatggcac cagaagtcat
     1921 cagaatgcaa gataaaaatc catacagctt tcagtcagat gtatatgcat ttggaattgt
     1981 tctgtatgaa ttgatgactg gacagttacc ttattcaaac atcaacaaca gggaccagat
     2041 aatttttatg gtgggacgag gatacctgtc tccagatctc agtaaggtac ggagtaactg
     2101 tccaaaagcc atgaagagat taatggcaga gtgcctcaaa aagaaaagag atgagagacc
     2161 actctttccc caaattctcg cctctattga gctgctggcc cgctcattgc caaaaattca
     2221 ccgcagtgca tcagaaccct ccttgaatcg ggctggtttc caaacagagg attttagtct
     2281 atatgcttgt gcttctccaa aaacacccat ccaggcaggg ggatatggtg cgtttcctgt
     2341 ccactagaac ccagctttct tgtacaaagt ggtgatatcc aattaaccct cactaaagga
     2401 agtgtttaat gtaagagaat gaaccctata gtgagtcgta ttaccaccca acttttctat
     2461 acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
     2521 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
     2581 gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     2641 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     2701 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     2761 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     2821 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     2881 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     2941 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     3001 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     3061 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     3121 acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatggt gagcaagggc
     3181 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     3241 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     3301 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     3361 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     3421 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     3481 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     3541 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     3601 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     3661 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     3721 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     3781 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     3841 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaatcga tagatcctaa
     3901 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3961 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4021 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4081 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4141 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4201 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4261 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4321 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4381 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4441 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     4501 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4561 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4621 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4681 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4741 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     4801 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     4861 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     4921 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5341 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6241 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6961 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7021 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7081 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7141 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7201 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     7261 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7321 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     7381 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     7441 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     7501 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     7561 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     7621 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     7681 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     7741 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     7801 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     7861 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     7921 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7981 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8041 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8101 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8161 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8221 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8281 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8341 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8401 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8461 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8521 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8581 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8641 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8701 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8761 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8821 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8881 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8941 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9001 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9061 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9121 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9181 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9241 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9301 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9361 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9421 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     9481 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
     9541 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     9601 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     9661 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
     9721 actagtcgtg aggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     9781 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     9841 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     9901 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     9961 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
    10021 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
    10081 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
    10141 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
    10201 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
    10261 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
    10321 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
    10381 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
    10441 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
    10501 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
    10561 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
    10621 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
    10681 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
    10741 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
    10801 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
    10861 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
//
