LOCUS       sequence-250641-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR P546S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-P546S
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    215..332
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        380..1558
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          611..1549
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1506..1526
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1578..1602
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1605..1676,1677..3539,3540..3608,3609..5237)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGESREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5239..5263)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5271..5289
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5314..5333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5315..5333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5339..5359)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5413..5454
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5475..6042
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5492..6044
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5636..5653)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5863..5882
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6056..6655
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6056..6075)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6552..6572
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6669..7257
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6722..6742)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7140..7151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7332..7565
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7684..7702)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7770..7874
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7875..7943,7944..8735)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8906..9494
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9395..9414
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9648..9665
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9905..9926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9941..9946,9947..9964,9965..9971)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9979..9995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9984..10006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10003..10019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10003..10019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10069..10088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10280..10913
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10960..11085
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11582..11815
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12000..12044
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
       61 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
      121 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
      181 cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      241 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      301 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      361 cagtttggac tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
      421 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
      481 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
      541 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
      601 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
      661 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
      721 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
      781 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
      841 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
      901 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
      961 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     1021 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     1081 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     1141 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     1201 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     1261 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     1321 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     1381 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     1441 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     1501 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag
     1561 cggccctgca gatatcaaca agtttgtaca aaaaagcagg caccatgcga ccctccggga
     1621 cggccggggc agcgctcctg gcgctgctgg ctgcgctctg cccggcgagt cgggctctgg
     1681 aggaaaagaa agtttgccaa ggcacgagta acaagctcac gcagttgggc acttttgaag
     1741 atcattttct cagcctccag aggatgttca ataactgtga ggtggtcctt gggaatttgg
     1801 aaattaccta tgtgcagagg aattatgatc tttccttctt aaagaccatc caggaggtgg
     1861 ctggttatgt cctcattgcc ctcaacacag tggagcgaat tcctttggaa aacctgcaga
     1921 tcatcagagg aaatatgtac tacgaaaatt cctatgcctt agcagtctta tctaactatg
     1981 atgcaaataa aaccggactg aaggagctgc ccatgagaaa tttacaggaa atcctgcatg
     2041 gcgccgtgcg gttcagcaac aaccctgccc tgtgcaacgt ggagagcatc cagtggcggg
     2101 acatagtcag cagtgacttt ctcagcaaca tgtcgatgga cttccagaac cacctgggca
     2161 gctgccaaaa gtgtgatcca agctgtccca atgggagctg ctggggtgca ggagaggaga
     2221 actgccagaa actgaccaaa atcatctgtg cccagcagtg ctccgggcgc tgccgtggca
     2281 agtcccccag tgactgctgc cacaaccagt gtgctgcagg ctgcacaggc ccccgggaga
     2341 gcgactgcct ggtctgccgc aaattccgag acgaagccac gtgcaaggac acctgccccc
     2401 cactcatgct ctacaacccc accacgtacc agatggatgt gaaccccgag ggcaaataca
     2461 gctttggtgc cacctgcgtg aagaagtgtc cccgtaatta tgtggtgaca gatcacggct
     2521 cgtgcgtccg agcctgtggg gccgacagct atgagatgga ggaagacggc gtccgcaagt
     2581 gtaagaagtg cgaagggcct tgccgcaaag tgtgtaacgg aataggtatt ggtgaattta
     2641 aagactcact ctccataaat gctacgaata ttaaacactt caaaaactgc acctccatca
     2701 gtggcgatct ccacatcctg ccggtggcat ttaggggtga ctccttcaca catactcctc
     2761 ctctggatcc acaggaactg gatattctga aaaccgtaaa ggaaatcaca gggtttttgc
     2821 tgattcaggc ttggcctgaa aacaggacgg acctccatgc ctttgagaac ctagaaatca
     2881 tacgcggcag gaccaagcaa catggtcagt tttctcttgc agtcgtcagc ctgaacataa
     2941 catccttggg attacgctcc ctcaaggaga taagtgatgg agatgtgata atttcaggaa
     3001 acaaaaattt gtgctatgca aatacaataa actggaaaaa actgtttggg acctccggtc
     3061 agaaaaccaa aattataagc aacagaggtg aaaacagctg caaggccaca ggccaggtct
     3121 gccatgcctt gtgctccccc gagggctgct ggggcccgga gcccagggac tgcgtctctt
     3181 gccggaatgt cagccgaggc agggaatgcg tggacaagtg caaccttctg gagggtgagt
     3241 caagggagtt tgtggagaac tctgagtgca tacagtgcca cccagagtgc ctgcctcagg
     3301 ccatgaacat cacctgcaca ggacggggac cagacaactg tatccagtgt gcccactaca
     3361 ttgacggccc ccactgcgtc aagacctgcc cggcaggagt catgggagaa aacaacaccc
     3421 tggtctggaa gtacgcagac gccggccatg tgtgccacct gtgccatcca aactgcacct
     3481 acggatgcac tgggccaggt cttgaaggct gtccaacgaa tgggcctaag atcccgtcca
     3541 tcgccactgg gatggtgggg gccctcctct tgctgctggt ggtggccctg gggatcggcc
     3601 tcttcatgcg aaggcgccac atcgttcgga agcgcacgct gcggaggctg ctgcaggaga
     3661 gggagcttgt ggagcctctt acacccagtg gagaagctcc caaccaagct ctcttgagga
     3721 tcttgaagga aactgaattc aaaaagatca aagtgctggg ctccggtgcg ttcggcacgg
     3781 tgtataaggg actctggatc ccagaaggtg agaaagttaa aattcccgtc gctatcaagg
     3841 aattaagaga agcaacatct ccgaaagcca acaaggaaat cctcgatgaa gcctacgtga
     3901 tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg catctgcctc acctccaccg
     3961 tgcagctcat cacgcagctc atgcccttcg gctgcctcct ggactatgtc cgggaacaca
     4021 aagacaatat tggctcccag tacctgctca actggtgtgt gcagatcgca aagggcatga
     4081 actacttgga ggaccgtcgc ttggtgcacc gcgacctggc agccaggaac gtactggtga
     4141 aaacaccgca gcatgtcaag atcacagatt ttgggctggc caaactgctg ggtgcggaag
     4201 agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa gtggatggca ttggaatcaa
     4261 ttttacacag aatctatacc caccagagtg atgtctggag ctacggggtg accgtttggg
     4321 agttgatgac ctttggatcc aagccatatg acggaatccc tgccagcgag atctcctcca
     4381 tcctggagaa aggagaacgc ctccctcagc cacccatatg taccatcgat gtctacatga
     4441 tcatggtcaa gtgctggatg atagacgcag atagtcgccc aaagttccgt gagttgatca
     4501 tcgaattctc caaaatggcc cgagaccccc agcgctacct tgtcattcag ggggatgaaa
     4561 gaatgcattt gccaagtcct acagactcca acttctaccg tgccctgatg gatgaagaag
     4621 acatggacga cgtggtggat gccgacgagt acctcatccc acagcagggc ttcttcagca
     4681 gcccctccac gtcacggact cccctcctga gctctctgag tgcaaccagc aacaattcca
     4741 ccgtggcttg cattgataga aatgggctgc aaagctgtcc catcaaggaa gacagcttct
     4801 tgcagcgata cagctcagac cccacaggcg ccttgactga ggacagcata gacgacacct
     4861 tcctcccagt gcctgaatac ataaaccagt ccgttcccaa aaggcccgct ggctctgtgc
     4921 agaatcctgt ctatcacaat cagcctctga accccgcgcc cagcagagac ccacactacc
     4981 aggaccccca cagcactgca gtgggcaacc ccgagtatct caacactgtc cagcccacct
     5041 gtgtcaacag cacattcgac agccctgccc actgggccca gaaaggcagc caccaaatta
     5101 gcctggacaa ccctgactac cagcaggact tctttcccaa ggaagccaag ccaaatggca
     5161 tctttaaggg ctccacagct gaaaatgcag aatacctaag ggtcgcgcca caaagcagtg
     5221 aatttattgg agcatagaac ccagctttct tgtacaaagt ggtgatatcc aattaaccct
     5281 cactaaaggg tttgtgtttg tataagttgt taaccctata gtgagtcgta ttaccaccca
     5341 acttttctat acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     5401 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     5461 ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     5521 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5581 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5641 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5701 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5761 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5821 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5881 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5941 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     6001 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatgac
     6061 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     6121 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     6181 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6241 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6301 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6361 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6421 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6481 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6541 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6601 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctaaatcga
     6661 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6721 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6781 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6841 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6901 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6961 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7021 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7081 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7141 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7201 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7261 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7321 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     7381 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7441 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7501 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7561 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7621 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     7681 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7741 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7801 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7861 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7921 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7981 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8041 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8101 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8161 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8221 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8281 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8341 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8401 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8461 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8521 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8581 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8641 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8701 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8761 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8821 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8881 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8941 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9001 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9061 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9121 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9181 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9241 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9301 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9361 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9421 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9481 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9541 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9601 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9661 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9721 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9781 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9841 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9901 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9961 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
    10021 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10081 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10141 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    10201 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10261 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    10321 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    10381 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    10441 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    10501 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    10561 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    10621 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    10681 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    10741 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10801 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10861 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    10921 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    10981 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    11041 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    11101 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    11161 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    11221 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    11281 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    11341 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    11401 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
    11461 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
    11521 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
    11581 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
    11641 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
    11701 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
    11761 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
    11821 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
    11881 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
    11941 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    12001 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    12061 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    12121 ta
//
