LOCUS       sequence-250651-       10707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3R1 I381Kfs*15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3R1-I381Kfs*15
FEATURES             Location/Qualifiers
     source          1..10707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3451..3477
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5644..5668)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5721..5762
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5783..6350
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5800..6352
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5944..5961)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6171..6190
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6364..6366,6367..6369,6370..7083)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6364..6366,6367..6369,6370..7080)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6409..6430)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6670..6689)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7017..7038
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7097..7685
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7150..7170)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7568..7579)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7760..7993
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8112..8130)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8198..8302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8303..8371,8372..9163)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8521..8540)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9334..9922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9823..9842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10076..10093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10333..10354
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10369..10374,10375..10392,10393..10399)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10407..10423
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10412..10434
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10431..10447
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10431..10447
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10497..10516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatggtt tacccatacg atgttccaga ttacgctagt
     3481 gctgaggggt accagtacag agcgctgtat gattataaaa aggaaagaga agaagatatt
     3541 gacttgcact tgggtgacat attgactgtg aataaagggt ccttagtagc tcttggattc
     3601 agtgatggac aggaagccag gcctgaagaa attggctggt taaatggcta taatgaaacc
     3661 acaggggaaa ggggggactt tccgggaact tatgtagaat atattggaag gaaaaaaatc
     3721 tcgcctccca caccaaagcc ccggccacct cggcctcttc ctgttgcacc aggttcttcg
     3781 aaaactgaag cagatgttga acaacaagct ttgactctcc cggatcttgc agagcagttt
     3841 gcccctcctg acattgcccc gcctcttctt atcaagctcg tggaagccat tgaaaagaaa
     3901 ggtctggaat gttcaactct atacagaaca cagagctcca gcaacctggc agaattacga
     3961 cagcttcttg attgtgatac accctccgtg gacttggaaa tgatcgatgt gcacgttttg
     4021 gctgacgctt tcaaacgcta tctcctggac ttaccaaatc ctgtcattcc agcagccgtt
     4081 tacagtgaaa tgatttcttt agctccagaa gtacaaagct ccgaagaata tattcagcta
     4141 ttgaagaagc ttattaggtc gcctagcata cctcatcagt attggcttac gcttcagtat
     4201 ttgttaaaac atttcttcaa gctctctcaa acctccagca aaaatctgtt gaatgcaaga
     4261 gtactctctg aaattttcag ccctatgctt ttcagattct cagcagccag ctctgataat
     4321 actgaaaacc tcataaaagt tatagaaatt ttaatctcaa ctgaatggaa tgaacgacag
     4381 cctgcaccag cactgcctcc taaaccacca aaacctacta ctgtagccaa caacggtatg
     4441 aataacaata tgtccttaca agatgctgaa tggtactggg gagatatctc gagggaagaa
     4501 gtgaatgaaa aacttcgaga tacagcagac gggacctttt tggtacgaga tgcgtctact
     4561 aaaatgcatg gtgattatac tcttacacta aggaaagggg gaaataacaa attaaaatat
     4621 ttcatcgaga tgggaaatat ggcttctctg acccattaac cttcagttct gtggttgaat
     4681 taataaacca ctaccggaat gaatctctag ctcagtataa tcccaaattg gatgtgaaat
     4741 tactttatcc agtatccaaa taccaacagg atcaagttgt caaagaagat aatattgaag
     4801 ctgtagggaa aaaattacat gaatataaca ctcagtttca agaaaaaagt cgagaatatg
     4861 atagattata tgaagaatat acccgcacat cccaggaaat ccaaatgaaa aggacagcta
     4921 ttgaagcatt taatgaaacc ataaaaatat ttgaagaaca gtgccagacc caagagcggt
     4981 acagcaaaga atacatagaa aagtttaaac gtgaaggcaa tgagaaagaa atacaaagga
     5041 ttatgcataa ttatgataag ttgaagtctc gaatcagtga aattattgac agtagaagaa
     5101 gattggaaga agacttgaag aagcaggcag ctgagtatcg agaaattgac aaacgtatga
     5161 acagcattaa accagacctt atccagctga gaaagacgag agaccaatac ttgatgtggt
     5221 tgactcaaaa aggtgttcgg caaaagaagt tgaacgagtg gttgggcaat gaaaacactg
     5281 aagaccaata ttcactggtg gaagatgatg aagatttgcc ccatcatgat gagaagacat
     5341 ggaatgttgg aagcagcaac cgaaacaaag ctgaaaacct gttgcgaggg aagcgagatg
     5401 gcacttttct tgtccgggag agcagtaaac agggctgcta tgcctgctct gtagtggtgg
     5461 acggcgaagt aaagcattgt gtcataaaca aaacagcaac tggctatggc tttgccgagc
     5521 cctataactt gtacagctct ctgaaagaac tggtgctaca ttaccaacac acctcccttg
     5581 tgcagcacaa cgactccctc aatgtcacac tagcctaccc agtatatgca cagcagaggc
     5641 gatgcccaac tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc
     5701 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     5761 cgcgtaccgg ttagtaatga gatccctccc ccccccctaa cgttactggc cgaagccgct
     5821 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5881 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5941 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     6001 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     6061 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     6121 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     6181 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     6241 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     6301 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     6361 catatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     6421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     6481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     6541 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     6601 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     6661 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     6721 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     6781 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     6841 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     6901 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     6961 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     7021 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     7081 taaatcgata gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     7141 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     7201 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     7261 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     7321 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     7381 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     7441 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     7501 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     7561 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     7621 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     7681 cccgcctgag atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     7741 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     7801 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     7861 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     7921 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     7981 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     8041 aaatgaatat cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg
     8101 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     8161 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     8221 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8281 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8341 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8401 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     8461 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     8521 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     8581 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     8641 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     8701 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     8761 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     8821 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     8881 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     8941 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     9001 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     9061 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     9121 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     9181 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     9241 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     9301 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     9361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     9421 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     9481 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     9541 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     9601 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     9661 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     9721 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     9781 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     9841 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     9901 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     9961 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
    10021 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
    10081 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
    10141 gccgattcat taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc
    10201 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
    10261 ggcttttgca aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga
    10321 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
    10381 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
    10441 ctatgacatg attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10501 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10561 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10621 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10681 ttaaatatgt actacaaact tagtagt
//
