LOCUS       sequence-250652-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR P699L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-P699L
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    271..388
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        436..1614
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          667..1605
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1562..1582
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1634..1658
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1661..1732,1733..3595,3596..3664,3665..5293)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEALNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5295..5319)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5327..5345
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5370..5389)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5371..5389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5395..5415)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5469..5510
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5531..6098
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5548..6100
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5692..5709)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5919..5938
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6112..6711
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6112..6131)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6608..6628
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6725..7313
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6778..6798)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7196..7207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7388..7621
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7740..7758)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7826..7930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7931..7999,8000..8791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8962..9550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9451..9470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9704..9721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9961..9982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9997..10002,10003..10020,10021..10027)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10035..10051
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10040..10062
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10059..10075
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10059..10075
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10125..10144
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10336..10969
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11016..11141
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11638..11871
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12056..12100
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
       61 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
      121 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
      181 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
      241 gaattcacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      301 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      361 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
      421 ttggactagt cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
      481 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
      541 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
      601 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
      661 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
      721 tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg
      781 gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct
      841 tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc
      901 gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg
      961 acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt
     1021 tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg
     1081 aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg
     1141 cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg
     1201 gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg
     1261 agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg
     1321 aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg
     1381 tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag
     1441 gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct
     1501 tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt
     1561 ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgaagcggc
     1621 cctgcagata tcaacaagtt tgtacaaaaa agcaggcacc atgcgaccct ccgggacggc
     1681 cggggcagcg ctcctggcgc tgctggctgc gctctgcccg gcgagtcggg ctctggagga
     1741 aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag ttgggcactt ttgaagatca
     1801 ttttctcagc ctccagagga tgttcaataa ctgtgaggtg gtccttggga atttggaaat
     1861 tacctatgtg cagaggaatt atgatctttc cttcttaaag accatccagg aggtggctgg
     1921 ttatgtcctc attgccctca acacagtgga gcgaattcct ttggaaaacc tgcagatcat
     1981 cagaggaaat atgtactacg aaaattccta tgccttagca gtcttatcta actatgatgc
     2041 aaataaaacc ggactgaagg agctgcccat gagaaattta caggaaatcc tgcatggcgc
     2101 cgtgcggttc agcaacaacc ctgccctgtg caacgtggag agcatccagt ggcgggacat
     2161 agtcagcagt gactttctca gcaacatgtc gatggacttc cagaaccacc tgggcagctg
     2221 ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg ggtgcaggag aggagaactg
     2281 ccagaaactg accaaaatca tctgtgccca gcagtgctcc gggcgctgcc gtggcaagtc
     2341 ccccagtgac tgctgccaca accagtgtgc tgcaggctgc acaggccccc gggagagcga
     2401 ctgcctggtc tgccgcaaat tccgagacga agccacgtgc aaggacacct gccccccact
     2461 catgctctac aaccccacca cgtaccagat ggatgtgaac cccgagggca aatacagctt
     2521 tggtgccacc tgcgtgaaga agtgtccccg taattatgtg gtgacagatc acggctcgtg
     2581 cgtccgagcc tgtggggccg acagctatga gatggaggaa gacggcgtcc gcaagtgtaa
     2641 gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata ggtattggtg aatttaaaga
     2701 ctcactctcc ataaatgcta cgaatattaa acacttcaaa aactgcacct ccatcagtgg
     2761 cgatctccac atcctgccgg tggcatttag gggtgactcc ttcacacata ctcctcctct
     2821 ggatccacag gaactggata ttctgaaaac cgtaaaggaa atcacagggt ttttgctgat
     2881 tcaggcttgg cctgaaaaca ggacggacct ccatgccttt gagaacctag aaatcatacg
     2941 cggcaggacc aagcaacatg gtcagttttc tcttgcagtc gtcagcctga acataacatc
     3001 cttgggatta cgctccctca aggagataag tgatggagat gtgataattt caggaaacaa
     3061 aaatttgtgc tatgcaaata caataaactg gaaaaaactg tttgggacct ccggtcagaa
     3121 aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag gccacaggcc aggtctgcca
     3181 tgccttgtgc tcccccgagg gctgctgggg cccggagccc agggactgcg tctcttgccg
     3241 gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac cttctggagg gtgagccaag
     3301 ggagtttgtg gagaactctg agtgcataca gtgccaccca gagtgcctgc ctcaggccat
     3361 gaacatcacc tgcacaggac ggggaccaga caactgtatc cagtgtgccc actacattga
     3421 cggcccccac tgcgtcaaga cctgcccggc aggagtcatg ggagaaaaca acaccctggt
     3481 ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc catccaaact gcacctacgg
     3541 atgcactggg ccaggtcttg aaggctgtcc aacgaatggg cctaagatcc cgtccatcgc
     3601 cactgggatg gtgggggccc tcctcttgct gctggtggtg gccctgggga tcggcctctt
     3661 catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg aggctgctgc aggagaggga
     3721 gcttgtggag cctcttacac ccagtggaga agctctcaac caagctctct tgaggatctt
     3781 gaaggaaact gaattcaaaa agatcaaagt gctgggctcc ggtgcgttcg gcacggtgta
     3841 taagggactc tggatcccag aaggtgagaa agttaaaatt cccgtcgcta tcaaggaatt
     3901 aagagaagca acatctccga aagccaacaa ggaaatcctc gatgaagcct acgtgatggc
     3961 cagcgtggac aacccccacg tgtgccgcct gctgggcatc tgcctcacct ccaccgtgca
     4021 gctcatcacg cagctcatgc ccttcggctg cctcctggac tatgtccggg aacacaaaga
     4081 caatattggc tcccagtacc tgctcaactg gtgtgtgcag atcgcaaagg gcatgaacta
     4141 cttggaggac cgtcgcttgg tgcaccgcga cctggcagcc aggaacgtac tggtgaaaac
     4201 accgcagcat gtcaagatca cagattttgg gctggccaaa ctgctgggtg cggaagagaa
     4261 agaataccat gcagaaggag gcaaagtgcc tatcaagtgg atggcattgg aatcaatttt
     4321 acacagaatc tatacccacc agagtgatgt ctggagctac ggggtgaccg tttgggagtt
     4381 gatgaccttt ggatccaagc catatgacgg aatccctgcc agcgagatct cctccatcct
     4441 ggagaaagga gaacgcctcc ctcagccacc catatgtacc atcgatgtct acatgatcat
     4501 ggtcaagtgc tggatgatag acgcagatag tcgcccaaag ttccgtgagt tgatcatcga
     4561 attctccaaa atggcccgag acccccagcg ctaccttgtc attcaggggg atgaaagaat
     4621 gcatttgcca agtcctacag actccaactt ctaccgtgcc ctgatggatg aagaagacat
     4681 ggacgacgtg gtggatgccg acgagtacct catcccacag cagggcttct tcagcagccc
     4741 ctccacgtca cggactcccc tcctgagctc tctgagtgca accagcaaca attccaccgt
     4801 ggcttgcatt gatagaaatg ggctgcaaag ctgtcccatc aaggaagaca gcttcttgca
     4861 gcgatacagc tcagacccca caggcgcctt gactgaggac agcatagacg acaccttcct
     4921 cccagtgcct gaatacataa accagtccgt tcccaaaagg cccgctggct ctgtgcagaa
     4981 tcctgtctat cacaatcagc ctctgaaccc cgcgcccagc agagacccac actaccagga
     5041 cccccacagc actgcagtgg gcaaccccga gtatctcaac actgtccagc ccacctgtgt
     5101 caacagcaca ttcgacagcc ctgcccactg ggcccagaaa ggcagccacc aaattagcct
     5161 ggacaaccct gactaccagc aggacttctt tcccaaggaa gccaagccaa atggcatctt
     5221 taagggctcc acagctgaaa atgcagaata cctaagggtc gcgccacaaa gcagtgaatt
     5281 tattggagca tagaacccag ctttcttgta caaagtggtg atatccaatt aaccctcact
     5341 aaagggaaga tattgaaaga atttgatgtc cctatagtga gtcgtattac cacccaactt
     5401 ttctatacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc
     5461 ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt
     5521 agtaatgaga tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     5581 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5641 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5701 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5761 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5821 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5881 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5941 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     6001 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     6061 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca tatgaccgag
     6121 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6181 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6241 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6301 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6361 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6421 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6481 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6541 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6601 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6661 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta aatcgataga
     6721 tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6781 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6841 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6901 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6961 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7021 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7081 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7141 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7201 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7261 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat
     7321 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7381 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     7441 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7501 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7561 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7621 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7681 gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg
     7741 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8161 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9601 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9661 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9721 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9781 atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9841 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9901 aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9961 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    10021 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
    10081 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10141 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    10201 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
    10261 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    10321 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
    10381 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
    10441 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
    10501 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
    10561 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
    10621 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
    10681 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
    10741 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
    10801 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10861 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10921 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
    10981 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
    11041 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
    11101 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
    11161 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
    11221 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
    11281 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
    11341 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
    11401 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
    11461 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
    11521 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    11581 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    11641 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    11701 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    11761 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    11821 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    11881 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    11941 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    12001 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    12061 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    12121 aa
//
