LOCUS       sequence-250653-       10910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF K601E.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-K601E
FEATURES             Location/Qualifiers
     source          1..10910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1389..1398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3693..3717)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3725..3743
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3768..3787)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3769..3787)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3793..3813)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3867..3908
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3929..4496
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3946..4498
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4090..4107)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4317..4336
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4510..4512,4513..4515,4516..5229)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4510..4512,4513..4515,4516..5226)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4555..4576)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4816..4835)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5163..5184
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5243..5831
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5296..5316)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5714..5725)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5906..6139
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6258..6276)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6344..6448
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6449..6517,6518..7309)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6667..6686)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7480..8068
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7969..7988
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8222..8239
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8479..8500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8515..8520,8521..8538,8539..8545)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8553..8569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8558..8580
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8577..8593
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8577..8593
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8643..8662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8854..9487
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9534..9659
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10156..10389
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10574..10618
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc
     1441 tctgttcaac ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc
     1501 ggctgcggac cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac
     1561 acaggaacat atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat
     1621 atatctggag gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca
     1681 acagttattg gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat
     1741 ggataccgtt acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt
     1801 ttttcaaaat cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt
     1861 tagagtcttc ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt
     1921 ccgagacagt ctaaagaaag cactgatgat gagaggtcta atcccagagt gctgtgctgt
     1981 ttacagaatt caggatggag agaagaaacc aattggttgg gacactgata tttcctggct
     2041 tactggagaa gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt
     2101 tgtacgaaaa acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca
     2161 gggtttccgc tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc
     2221 actgatgtgt gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca
     2281 ccacccaata ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc
     2341 cccttccgca cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc
     2401 aaaatccatt ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt
     2461 tgggcaacga gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt
     2521 caatattgat gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg
     2581 tttgtctgct accccccctg cctcattacc tggctcacta actaacgtga aagccttaca
     2641 gaaatctcca ggacctcagc gagaaaggaa gtcatcttca tcctcagaag acaggaatcg
     2701 aatgaaaaca cttggtagac gggactcgag tgatgattgg gagattcctg atgggcagat
     2761 tacagtggga caaagaattg gatctggatc atttggaaca gtctacaagg gaaagtggca
     2821 tggtgatgtg gcagtgaaaa tgttgaatgt gacagcacct acacctcagc agttacaagc
     2881 cttcaaaaat gaagtaggag tactcaggaa aacacgacat gtgaatatcc tactcttcat
     2941 gggctattcc acaaagccac aactggctat tgttacccag tggtgtgagg gctccagctt
     3001 gtatcaccat ctccatatca ttgagaccaa atttgagatg atcaaactta tagatattgc
     3061 acgacagact gcacagggca tggattactt acacgccaag tcaatcatcc acagagacct
     3121 caagagtaat aatatatttc ttcatgaaga cctcacagta aaaataggtg attttggtct
     3181 agctacagtg gaatctcgat ggagtgggtc ccatcagttt gaacagttgt ctggatccat
     3241 tttgtggatg gcaccagaag tcatcagaat gcaagataaa aatccataca gctttcagtc
     3301 agatgtatat gcatttggaa ttgttctgta tgaattgatg actggacagt taccttattc
     3361 aaacatcaac aacagggacc agataatttt tatggtggga cgaggatacc tgtctccaga
     3421 tctcagtaag gtacggagta actgtccaaa agccatgaag agattaatgg cagagtgcct
     3481 caaaaagaaa agagatgaga gaccactctt tccccaaatt ctcgcctcta ttgagctgct
     3541 ggcccgctca ttgccaaaaa ttcaccgcag tgcatcagaa ccctccttga atcgggctgg
     3601 tttccaaaca gaggatttta gtctatatgc ttgtgcttct ccaaaaacac ccatccaggc
     3661 agggggatat ggtgcgtttc ctgtccacta gaacccagct ttcttgtaca aagtggtgat
     3721 atccaattaa ccctcactaa aggaataatg ttgtgaaata gatgtgaccc tatagtgagt
     3781 cgtattacca cccaactttt ctatacaaag tggttgatat ccagcacagt ggcggccgct
     3841 cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg
     3901 attctacgcg taccggttag taatgagatc cctccccccc ccntaacgtt actggccgaa
     3961 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
     4021 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
     4081 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
     4141 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
     4201 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
     4261 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
     4321 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
     4381 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
     4441 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataatatg
     4501 gccacacata tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     4561 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     4621 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     4681 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     4741 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     4801 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     4861 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4921 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     4981 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     5041 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     5101 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     5161 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     5221 tacaagtaaa tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     5281 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5341 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     5401 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     5461 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     5521 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     5581 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     5641 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     5701 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     5761 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     5821 cgcctccccg cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc
     5881 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     5941 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6001 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6061 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6121 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6181 gcaaagaaat gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca
     6241 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6301 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     6361 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     6421 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6481 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6541 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6601 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6661 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     6721 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6781 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6841 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6901 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6961 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     7021 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     7081 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     7141 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7201 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7261 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7321 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7381 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7441 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7501 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7561 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7621 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7681 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     8041 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     8101 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     8161 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8221 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8281 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca
     8341 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8401 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg
     8461 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     8521 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     8581 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag
     8641 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     8701 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     8761 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     8821 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     8881 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     8941 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
     9001 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
     9061 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
     9121 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
     9181 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
     9241 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
     9301 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9361 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9421 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9481 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     9541 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     9601 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     9661 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     9721 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     9781 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     9841 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     9901 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     9961 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
    10021 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
    10081 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
    10141 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
    10201 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
    10261 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
    10321 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    10381 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    10441 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    10501 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    10561 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    10621 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    10681 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    10741 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
    10801 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    10861 cggatctcga cggtatcgcc gaattcacaa atggcagtat tcatccacaa
//
