LOCUS       sequence-250655-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF K601Q.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-K601Q
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1226
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          279..1217
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1174..1194
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1246..1270
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1271..1280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3575..3599)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3652..3693
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3714..4281
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3731..4283
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3875..3892)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4102..4121
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4295..4297,4298..4300,4301..5014)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4295..4297,4298..4300,4301..5011)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4340..4361)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4601..4620)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4948..4969
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5028..5616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5081..5101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5499..5510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5691..5924
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6043..6061)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6129..6233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6234..6302,6303..7094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6452..6471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7265..7853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7754..7773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8007..8024
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8264..8285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8300..8305,8306..8323,8324..8330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8338..8354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8343..8365
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8362..8378
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8362..8378
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8428..8447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8639..9272
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9319..9444
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9941..10174
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10359..10403
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10696..10813
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc
       61 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      121 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      181 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      241 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      301 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      361 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      421 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      481 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      541 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      601 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      661 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      721 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      781 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      841 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
      901 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
      961 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1021 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1081 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1141 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1201 tttttcttcc atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa
     1261 aaagcaggca ccatggcggc gctgagcggt ggcggtggtg gcggcgcgga gccgggccag
     1321 gctctgttca acggggacat ggagcccgag gccggcgccg gcgccggcgc cgcggcctct
     1381 tcggctgcgg accctgccat tccggaggag gtgtggaata tcaaacaaat gattaagttg
     1441 acacaggaac atatagaggc cctattggac aaatttggtg gggagcataa tccaccatca
     1501 atatatctgg aggcctatga agaatacacc agcaagctag atgcactcca acaaagagaa
     1561 caacagttat tggaatctct ggggaacgga actgattttt ctgtttctag ctctgcatca
     1621 atggataccg ttacatcttc ttcctcttct agcctttcag tgctaccttc atctctttca
     1681 gtttttcaaa atcccacaga tgtggcacgg agcaacccca agtcaccaca aaaacctatc
     1741 gttagagtct tcctgcccaa caaacagagg acagtggtac ctgcaaggtg tggagttaca
     1801 gtccgagaca gtctaaagaa agcactgatg atgagaggtc taatcccaga gtgctgtgct
     1861 gtttacagaa ttcaggatgg agagaagaaa ccaattggtt gggacactga tatttcctgg
     1921 cttactggag aagaattgca tgtggaagtg ttggagaatg ttccacttac aacacacaac
     1981 tttgtacgaa aaacgttttt caccttagca ttttgtgact tttgtcgaaa gctgcttttc
     2041 cagggtttcc gctgtcaaac atgtggttat aaatttcacc agcgttgtag tacagaagtt
     2101 ccactgatgt gtgttaatta tgaccaactt gatttgctgt ttgtctccaa gttctttgaa
     2161 caccacccaa taccacagga agaggcgtcc ttagcagaga ctgccctaac atctggatca
     2221 tccccttccg cacccgcctc ggactctatt gggccccaaa ttctcaccag tccgtctcct
     2281 tcaaaatcca ttccaattcc acagcccttc cgaccagcag atgaagatca tcgaaatcaa
     2341 tttgggcaac gagaccgatc ctcatcagct cccaatgtgc atataaacac aatagaacct
     2401 gtcaatattg atgacttgat tagagaccaa ggatttcgtg gtgatggagg atcaaccaca
     2461 ggtttgtctg ctaccccccc tgcctcatta cctggctcac taactaacgt gaaagcctta
     2521 cagaaatctc caggacctca gcgagaaagg aagtcatctt catcctcaga agacaggaat
     2581 cgaatgaaaa cacttggtag acgggactcg agtgatgatt gggagattcc tgatgggcag
     2641 attacagtgg gacaaagaat tggatctgga tcatttggaa cagtctacaa gggaaagtgg
     2701 catggtgatg tggcagtgaa aatgttgaat gtgacagcac ctacacctca gcagttacaa
     2761 gccttcaaaa atgaagtagg agtactcagg aaaacacgac atgtgaatat cctactcttc
     2821 atgggctatt ccacaaagcc acaactggct attgttaccc agtggtgtga gggctccagc
     2881 ttgtatcacc atctccatat cattgagacc aaatttgaga tgatcaaact tatagatatt
     2941 gcacgacaga ctgcacaggg catggattac ttacacgcca agtcaatcat ccacagagac
     3001 ctcaagagta ataatatatt tcttcatgaa gacctcacag taaaaatagg tgattttggt
     3061 ctagctacag tgcaatctcg atggagtggg tcccatcagt ttgaacagtt gtctggatcc
     3121 attttgtgga tggcaccaga agtcatcaga atgcaagata aaaatccata cagctttcag
     3181 tcagatgtat atgcatttgg aattgttctg tatgaattga tgactggaca gttaccttat
     3241 tcaaacatca acaacaggga ccagataatt tttatggtgg gacgaggata cctgtctcca
     3301 gatctcagta aggtacggag taactgtcca aaagccatga agagattaat ggcagagtgc
     3361 ctcaaaaaga aaagagatga gagaccactc tttccccaaa ttctcgcctc tattgagctg
     3421 ctggcccgct cattgccaaa aattcaccgc agtgcatcag aaccctcctt gaatcgggct
     3481 ggtttccaaa cagaggattt tagtctatat gcttgtgctt ctccaaaaac acccatccag
     3541 gcagggggat atggtgcgtt tcctgtccac tagaacccag ctttcttgta caaagtggtt
     3601 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
     3661 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agatccctcc
     3721 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     3781 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     3841 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     3901 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3961 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     4021 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     4081 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     4141 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     4201 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     4261 ctttgaaaaa cacgatgata atatggccac acatatggtg agcaagggcg aggagctgtt
     4321 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4381 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4441 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     4501 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4561 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     4621 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     4681 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     4741 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     4801 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     4861 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     4921 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4981 gatcactctc ggcatggacg agctgtacaa gtaaatcgat agatcctaat caacctctgg
     5041 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5101 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5161 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5221 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5281 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5341 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5401 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5461 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5521 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5581 agacgagtcg gatctccctt tgggccgcct ccccgcctga gatcctttaa gaccaatgac
     5641 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     5701 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     5761 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5821 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5881 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5941 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggcccgg
     6001 gttaattaag gaaagggcta gatcattctt gaagacgaaa gggcctcgtg atacgcctat
     6061 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     6121 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6181 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     6241 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     6301 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     6361 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     6421 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
     6481 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     6541 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     6601 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     6661 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     6721 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     6781 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6841 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6901 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6961 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     7021 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     7081 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     7141 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     7201 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7261 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7321 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7381 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7441 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7501 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     7561 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     7621 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     7681 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     7741 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     7801 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     7861 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     7921 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     7981 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8041 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg
     8101 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8161 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg
     8221 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     8281 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     8341 tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat ttcacaaata
     8401 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     8461 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     8521 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     8581 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     8641 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     8701 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     8761 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     8821 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     8881 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     8941 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     9001 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     9061 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     9121 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9181 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9241 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     9301 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     9361 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     9421 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     9481 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     9541 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     9601 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     9661 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     9721 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     9781 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     9841 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     9901 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     9961 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
    10021 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    10081 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    10141 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    10201 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    10261 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
    10321 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
    10381 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
    10441 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
    10501 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
    10561 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
    10621 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccgaatt cacaaatggc
    10681 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    10741 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
    10801 aattcaaaat ttt
//
