LOCUS       sequence-250659-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR P699S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-P699S
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    139..256
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        304..1482
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          535..1473
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1430..1450
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1502..1526
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1529..1600,1601..3463,3464..3532,3533..5161)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEASNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5163..5187)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5195..5213
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5238..5257)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5239..5257)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5263..5283)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5337..5378
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5399..5966
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5416..5968
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5560..5577)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5787..5806
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5980..6579
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5980..5999)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6476..6496
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6593..7181
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6646..6666)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7064..7075)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7256..7489
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7608..7626)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7694..7798
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7799..7867,7868..8659)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8830..9418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9319..9338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9572..9589
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9829..9850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9865..9870,9871..9888,9889..9895)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9903..9919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9908..9930
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9927..9943
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9927..9943
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9993..10012
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10204..10837
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10884..11009
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11506..11739
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11924..11968
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
       61 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat
      121 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      181 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      241 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg
      301 tgaggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
      361 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
      421 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
      481 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
      541 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
      601 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      661 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      721 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      781 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      841 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      901 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      961 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     1021 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     1081 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     1141 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     1201 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     1261 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1321 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1381 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1441 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaagcggccc tgcagatatc
     1501 aacaagtttg tacaaaaaag caggcaccat gcgaccctcc gggacggccg gggcagcgct
     1561 cctggcgctg ctggctgcgc tctgcccggc gagtcgggct ctggaggaaa agaaagtttg
     1621 ccaaggcacg agtaacaagc tcacgcagtt gggcactttt gaagatcatt ttctcagcct
     1681 ccagaggatg ttcaataact gtgaggtggt ccttgggaat ttggaaatta cctatgtgca
     1741 gaggaattat gatctttcct tcttaaagac catccaggag gtggctggtt atgtcctcat
     1801 tgccctcaac acagtggagc gaattccttt ggaaaacctg cagatcatca gaggaaatat
     1861 gtactacgaa aattcctatg ccttagcagt cttatctaac tatgatgcaa ataaaaccgg
     1921 actgaaggag ctgcccatga gaaatttaca ggaaatcctg catggcgccg tgcggttcag
     1981 caacaaccct gccctgtgca acgtggagag catccagtgg cgggacatag tcagcagtga
     2041 ctttctcagc aacatgtcga tggacttcca gaaccacctg ggcagctgcc aaaagtgtga
     2101 tccaagctgt cccaatggga gctgctgggg tgcaggagag gagaactgcc agaaactgac
     2161 caaaatcatc tgtgcccagc agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg
     2221 ctgccacaac cagtgtgctg caggctgcac aggcccccgg gagagcgact gcctggtctg
     2281 ccgcaaattc cgagacgaag ccacgtgcaa ggacacctgc cccccactca tgctctacaa
     2341 ccccaccacg taccagatgg atgtgaaccc cgagggcaaa tacagctttg gtgccacctg
     2401 cgtgaagaag tgtccccgta attatgtggt gacagatcac ggctcgtgcg tccgagcctg
     2461 tggggccgac agctatgaga tggaggaaga cggcgtccgc aagtgtaaga agtgcgaagg
     2521 gccttgccgc aaagtgtgta acggaatagg tattggtgaa tttaaagact cactctccat
     2581 aaatgctacg aatattaaac acttcaaaaa ctgcacctcc atcagtggcg atctccacat
     2641 cctgccggtg gcatttaggg gtgactcctt cacacatact cctcctctgg atccacagga
     2701 actggatatt ctgaaaaccg taaaggaaat cacagggttt ttgctgattc aggcttggcc
     2761 tgaaaacagg acggacctcc atgcctttga gaacctagaa atcatacgcg gcaggaccaa
     2821 gcaacatggt cagttttctc ttgcagtcgt cagcctgaac ataacatcct tgggattacg
     2881 ctccctcaag gagataagtg atggagatgt gataatttca ggaaacaaaa atttgtgcta
     2941 tgcaaataca ataaactgga aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat
     3001 aagcaacaga ggtgaaaaca gctgcaaggc cacaggccag gtctgccatg ccttgtgctc
     3061 ccccgagggc tgctggggcc cggagcccag ggactgcgtc tcttgccgga atgtcagccg
     3121 aggcagggaa tgcgtggaca agtgcaacct tctggagggt gagccaaggg agtttgtgga
     3181 gaactctgag tgcatacagt gccacccaga gtgcctgcct caggccatga acatcacctg
     3241 cacaggacgg ggaccagaca actgtatcca gtgtgcccac tacattgacg gcccccactg
     3301 cgtcaagacc tgcccggcag gagtcatggg agaaaacaac accctggtct ggaagtacgc
     3361 agacgccggc catgtgtgcc acctgtgcca tccaaactgc acctacggat gcactgggcc
     3421 aggtcttgaa ggctgtccaa cgaatgggcc taagatcccg tccatcgcca ctgggatggt
     3481 gggggccctc ctcttgctgc tggtggtggc cctggggatc ggcctcttca tgcgaaggcg
     3541 ccacatcgtt cggaagcgca cgctgcggag gctgctgcag gagagggagc ttgtggagcc
     3601 tcttacaccc agtggagaag cttccaacca agctctcttg aggatcttga aggaaactga
     3661 attcaaaaag atcaaagtgc tgggctccgg tgcgttcggc acggtgtata agggactctg
     3721 gatcccagaa ggtgagaaag ttaaaattcc cgtcgctatc aaggaattaa gagaagcaac
     3781 atctccgaaa gccaacaagg aaatcctcga tgaagcctac gtgatggcca gcgtggacaa
     3841 cccccacgtg tgccgcctgc tgggcatctg cctcacctcc accgtgcagc tcatcacgca
     3901 gctcatgccc ttcggctgcc tcctggacta tgtccgggaa cacaaagaca atattggctc
     3961 ccagtacctg ctcaactggt gtgtgcagat cgcaaagggc atgaactact tggaggaccg
     4021 tcgcttggtg caccgcgacc tggcagccag gaacgtactg gtgaaaacac cgcagcatgt
     4081 caagatcaca gattttgggc tggccaaact gctgggtgcg gaagagaaag aataccatgc
     4141 agaaggaggc aaagtgccta tcaagtggat ggcattggaa tcaattttac acagaatcta
     4201 tacccaccag agtgatgtct ggagctacgg ggtgaccgtt tgggagttga tgacctttgg
     4261 atccaagcca tatgacggaa tccctgccag cgagatctcc tccatcctgg agaaaggaga
     4321 acgcctccct cagccaccca tatgtaccat cgatgtctac atgatcatgg tcaagtgctg
     4381 gatgatagac gcagatagtc gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat
     4441 ggcccgagac ccccagcgct accttgtcat tcagggggat gaaagaatgc atttgccaag
     4501 tcctacagac tccaacttct accgtgccct gatggatgaa gaagacatgg acgacgtggt
     4561 ggatgccgac gagtacctca tcccacagca gggcttcttc agcagcccct ccacgtcacg
     4621 gactcccctc ctgagctctc tgagtgcaac cagcaacaat tccaccgtgg cttgcattga
     4681 tagaaatggg ctgcaaagct gtcccatcaa ggaagacagc ttcttgcagc gatacagctc
     4741 agaccccaca ggcgccttga ctgaggacag catagacgac accttcctcc cagtgcctga
     4801 atacataaac cagtccgttc ccaaaaggcc cgctggctct gtgcagaatc ctgtctatca
     4861 caatcagcct ctgaaccccg cgcccagcag agacccacac taccaggacc cccacagcac
     4921 tgcagtgggc aaccccgagt atctcaacac tgtccagccc acctgtgtca acagcacatt
     4981 cgacagccct gcccactggg cccagaaagg cagccaccaa attagcctgg acaaccctga
     5041 ctaccagcag gacttctttc ccaaggaagc caagccaaat ggcatcttta agggctccac
     5101 agctgaaaat gcagaatacc taagggtcgc gccacaaagc agtgaattta ttggagcata
     5161 gaacccagct ttcttgtaca aagtggtgat atccaattaa ccctcactaa aggaattaga
     5221 agtaagtaga gtttaagccc tatagtgagt cgtattacca cccaactttt ctatacaaag
     5281 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta
     5341 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgagatc
     5401 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5461 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5521 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5581 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5641 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5701 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5761 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5821 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5881 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5941 ttttcctttg aaaaacacga tgataatatg gccacacata tgaccgagta caagcccacg
     6001 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     6061 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     6121 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     6181 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     6241 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     6301 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     6361 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     6421 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     6481 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6541 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc
     6601 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     6661 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     6721 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     6781 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     6841 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     6901 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     6961 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7021 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7081 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7141 ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca
     7201 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7261 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     7321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     7501 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     7561 cccgggttaa ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg
     7621 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     7681 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     7741 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     7801 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     7861 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     7921 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     7981 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8041 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8101 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8161 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8221 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     8281 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     8341 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     8401 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     8461 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     8521 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     8581 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     8641 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     8701 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8761 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8821 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8881 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8941 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9001 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9061 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     9121 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     9181 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     9241 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     9301 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9361 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9421 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     9481 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     9541 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     9601 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct
     9661 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9721 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg
     9781 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9841 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9901 aattgtgagc ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac
     9961 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    10021 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
    10081 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
    10141 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
    10201 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
    10261 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
    10321 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
    10381 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
    10441 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
    10501 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
    10561 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
    10621 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
    10681 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    10741 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    10801 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
    10861 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
    10921 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
    10981 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
    11041 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
    11101 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
    11161 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
    11221 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    11281 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    11341 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    11401 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    11461 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    11521 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    11581 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    11641 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    11701 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    11761 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    11821 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    11881 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    11941 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    12001 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    12061 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    12121 cc
//
