LOCUS       sequence-250665-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR P772R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-P772R
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    190..307
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        355..1533
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          586..1524
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1481..1501
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1553..1577
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1580..1651,1652..3514,3515..3583,3584..5212)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNRHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5214..5238)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5246..5264
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5289..5308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5290..5308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5314..5334)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5388..5429
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5450..6017
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5467..6019
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5611..5628)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5838..5857
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6031..6630
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6031..6050)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6527..6547
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6644..7232
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6697..6717)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7115..7126)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7307..7540
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7659..7677)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7745..7849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7850..7918,7919..8710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8881..9469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9370..9389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9623..9640
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9880..9901
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9916..9921,9922..9939,9940..9946)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9954..9970
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9959..9981
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9978..9994
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9978..9994
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10044..10063
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10255..10888
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10935..11060
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11557..11790
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11975..12019
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
       61 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
      121 gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa tggcagtatt
      181 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
      241 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
      301 aaattttcgg gtttattaca gggacagcag agatccagtt tggactagtc gtgaggctcc
      361 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      421 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      481 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      541 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
      601 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
      661 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
      721 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
      781 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
      841 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
      901 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
      961 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     1021 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     1081 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     1141 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     1201 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     1261 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     1321 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     1381 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     1441 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     1501 caaagttttt ttcttccatt tcaggtgtcg tgaagcggcc ctgcagatat caacaagttt
     1561 gtacaaaaaa gcaggcacca tgcgaccctc cgggacggcc ggggcagcgc tcctggcgct
     1621 gctggctgcg ctctgcccgg cgagtcgggc tctggaggaa aagaaagttt gccaaggcac
     1681 gagtaacaag ctcacgcagt tgggcacttt tgaagatcat tttctcagcc tccagaggat
     1741 gttcaataac tgtgaggtgg tccttgggaa tttggaaatt acctatgtgc agaggaatta
     1801 tgatctttcc ttcttaaaga ccatccagga ggtggctggt tatgtcctca ttgccctcaa
     1861 cacagtggag cgaattcctt tggaaaacct gcagatcatc agaggaaata tgtactacga
     1921 aaattcctat gccttagcag tcttatctaa ctatgatgca aataaaaccg gactgaagga
     1981 gctgcccatg agaaatttac aggaaatcct gcatggcgcc gtgcggttca gcaacaaccc
     2041 tgccctgtgc aacgtggaga gcatccagtg gcgggacata gtcagcagtg actttctcag
     2101 caacatgtcg atggacttcc agaaccacct gggcagctgc caaaagtgtg atccaagctg
     2161 tcccaatggg agctgctggg gtgcaggaga ggagaactgc cagaaactga ccaaaatcat
     2221 ctgtgcccag cagtgctccg ggcgctgccg tggcaagtcc cccagtgact gctgccacaa
     2281 ccagtgtgct gcaggctgca caggcccccg ggagagcgac tgcctggtct gccgcaaatt
     2341 ccgagacgaa gccacgtgca aggacacctg ccccccactc atgctctaca accccaccac
     2401 gtaccagatg gatgtgaacc ccgagggcaa atacagcttt ggtgccacct gcgtgaagaa
     2461 gtgtccccgt aattatgtgg tgacagatca cggctcgtgc gtccgagcct gtggggccga
     2521 cagctatgag atggaggaag acggcgtccg caagtgtaag aagtgcgaag ggccttgccg
     2581 caaagtgtgt aacggaatag gtattggtga atttaaagac tcactctcca taaatgctac
     2641 gaatattaaa cacttcaaaa actgcacctc catcagtggc gatctccaca tcctgccggt
     2701 ggcatttagg ggtgactcct tcacacatac tcctcctctg gatccacagg aactggatat
     2761 tctgaaaacc gtaaaggaaa tcacagggtt tttgctgatt caggcttggc ctgaaaacag
     2821 gacggacctc catgcctttg agaacctaga aatcatacgc ggcaggacca agcaacatgg
     2881 tcagttttct cttgcagtcg tcagcctgaa cataacatcc ttgggattac gctccctcaa
     2941 ggagataagt gatggagatg tgataatttc aggaaacaaa aatttgtgct atgcaaatac
     3001 aataaactgg aaaaaactgt ttgggacctc cggtcagaaa accaaaatta taagcaacag
     3061 aggtgaaaac agctgcaagg ccacaggcca ggtctgccat gccttgtgct cccccgaggg
     3121 ctgctggggc ccggagccca gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga
     3181 atgcgtggac aagtgcaacc ttctggaggg tgagccaagg gagtttgtgg agaactctga
     3241 gtgcatacag tgccacccag agtgcctgcc tcaggccatg aacatcacct gcacaggacg
     3301 gggaccagac aactgtatcc agtgtgccca ctacattgac ggcccccact gcgtcaagac
     3361 ctgcccggca ggagtcatgg gagaaaacaa caccctggtc tggaagtacg cagacgccgg
     3421 ccatgtgtgc cacctgtgcc atccaaactg cacctacgga tgcactgggc caggtcttga
     3481 aggctgtcca acgaatgggc ctaagatccc gtccatcgcc actgggatgg tgggggccct
     3541 cctcttgctg ctggtggtgg ccctggggat cggcctcttc atgcgaaggc gccacatcgt
     3601 tcggaagcgc acgctgcgga ggctgctgca ggagagggag cttgtggagc ctcttacacc
     3661 cagtggagaa gctcccaacc aagctctctt gaggatcttg aaggaaactg aattcaaaaa
     3721 gatcaaagtg ctgggctccg gtgcgttcgg cacggtgtat aagggactct ggatcccaga
     3781 aggtgagaaa gttaaaattc ccgtcgctat caaggaatta agagaagcaa catctccgaa
     3841 agccaacaag gaaatcctcg atgaagccta cgtgatggcc agcgtggaca accgccacgt
     3901 gtgccgcctg ctgggcatct gcctcacctc caccgtgcag ctcatcacgc agctcatgcc
     3961 cttcggctgc ctcctggact atgtccggga acacaaagac aatattggct cccagtacct
     4021 gctcaactgg tgtgtgcaga tcgcaaaggg catgaactac ttggaggacc gtcgcttggt
     4081 gcaccgcgac ctggcagcca ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac
     4141 agattttggg ctggccaaac tgctgggtgc ggaagagaaa gaataccatg cagaaggagg
     4201 caaagtgcct atcaagtgga tggcattgga atcaatttta cacagaatct atacccacca
     4261 gagtgatgtc tggagctacg gggtgaccgt ttgggagttg atgacctttg gatccaagcc
     4321 atatgacgga atccctgcca gcgagatctc ctccatcctg gagaaaggag aacgcctccc
     4381 tcagccaccc atatgtacca tcgatgtcta catgatcatg gtcaagtgct ggatgataga
     4441 cgcagatagt cgcccaaagt tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga
     4501 cccccagcgc taccttgtca ttcaggggga tgaaagaatg catttgccaa gtcctacaga
     4561 ctccaacttc taccgtgccc tgatggatga agaagacatg gacgacgtgg tggatgccga
     4621 cgagtacctc atcccacagc agggcttctt cagcagcccc tccacgtcac ggactcccct
     4681 cctgagctct ctgagtgcaa ccagcaacaa ttccaccgtg gcttgcattg atagaaatgg
     4741 gctgcaaagc tgtcccatca aggaagacag cttcttgcag cgatacagct cagaccccac
     4801 aggcgccttg actgaggaca gcatagacga caccttcctc ccagtgcctg aatacataaa
     4861 ccagtccgtt cccaaaaggc ccgctggctc tgtgcagaat cctgtctatc acaatcagcc
     4921 tctgaacccc gcgcccagca gagacccaca ctaccaggac ccccacagca ctgcagtggg
     4981 caaccccgag tatctcaaca ctgtccagcc cacctgtgtc aacagcacat tcgacagccc
     5041 tgcccactgg gcccagaaag gcagccacca aattagcctg gacaaccctg actaccagca
     5101 ggacttcttt cccaaggaag ccaagccaaa tggcatcttt aagggctcca cagctgaaaa
     5161 tgcagaatac ctaagggtcg cgccacaaag cagtgaattt attggagcat agaacccagc
     5221 tttcttgtac aaagtggtga tatccaatta accctcacta aaggtgattt agtatgtatt
     5281 agagttgacc ctatagtgag tcgtattacc acccaacttt tctatacaaa gtggttgata
     5341 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     5401 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagat ccctcccccc
     5461 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     5521 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     5581 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     5641 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     5701 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     5761 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     5821 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     5881 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     5941 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6001 gaaaaacacg atgataatat ggccacacat atgaccgagt acaagcccac ggtgcgcctc
     6061 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6121 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6181 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6241 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6301 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6361 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6421 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6481 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6541 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6601 tggtgcatga cccgcaagcc cggtgcctaa atcgatagat cctaatcaac ctctggatta
     6661 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6721 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6781 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     6841 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     6901 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     6961 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7021 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     7081 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7141 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7201 gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac
     7261 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     7321 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     7381 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7441 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7501 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     7561 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta
     7621 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
     7681 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7741 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7801 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7861 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7921 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7981 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8041 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     8101 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8161 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8221 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8281 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8341 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8401 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8461 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8521 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8581 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8641 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8701 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8761 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8821 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8881 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8941 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9001 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9061 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9121 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9181 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9241 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9301 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9361 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9421 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9481 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9541 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9601 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9661 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
     9721 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9781 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
     9841 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     9901 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     9961 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
    10021 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    10081 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    10141 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    10201 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
    10261 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
    10321 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
    10381 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
    10441 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
    10501 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
    10561 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
    10621 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
    10681 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
    10741 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10801 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10861 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    10921 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    10981 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    11041 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    11101 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    11161 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    11221 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    11281 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    11341 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    11401 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    11461 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    11521 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    11581 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    11641 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    11701 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    11761 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    11821 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    11881 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    11941 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    12001 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    12061 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    12121 ta
//
