LOCUS       sequence-250668-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR P848L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-P848L
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    69..186
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        234..1412
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          465..1403
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1360..1380
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1432..1456
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1459..1530,1531..3393,3394..3462,3463..5091)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTLQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5093..5117)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5125..5143
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5168..5187)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5169..5187)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5193..5213)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5267..5308
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5329..5896
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5346..5898
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5490..5507)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5717..5736
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5910..6509
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5910..5929)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6406..6426
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6523..7111
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6576..6596)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6994..7005)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7186..7419
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7538..7556)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7624..7728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7729..7797,7798..8589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8760..9348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9249..9268
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9502..9519
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9759..9780
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9795..9800,9801..9818,9819..9825)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9833..9849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9838..9860
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9857..9873
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9857..9873
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9923..9942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10134..10767
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10814..10939
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11436..11669
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11854..11898
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 atccattcga ttagtgaacg gatctcgacg gtatcgccga attcacaaat ggcagtattc
       61 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
      121 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
      181 aattttcggg tttattacag ggacagcaga gatccagttt ggactagtcg tgaggctccg
      241 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
      301 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
      361 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
      421 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
      481 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
      541 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
      601 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
      661 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
      721 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
      781 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
      841 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
      901 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
      961 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     1021 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     1081 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     1141 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     1201 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     1261 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     1321 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     1381 aaagtttttt tcttccattt caggtgtcgt gaagcggccc tgcagatatc aacaagtttg
     1441 tacaaaaaag caggcaccat gcgaccctcc gggacggccg gggcagcgct cctggcgctg
     1501 ctggctgcgc tctgcccggc gagtcgggct ctggaggaaa agaaagtttg ccaaggcacg
     1561 agtaacaagc tcacgcagtt gggcactttt gaagatcatt ttctcagcct ccagaggatg
     1621 ttcaataact gtgaggtggt ccttgggaat ttggaaatta cctatgtgca gaggaattat
     1681 gatctttcct tcttaaagac catccaggag gtggctggtt atgtcctcat tgccctcaac
     1741 acagtggagc gaattccttt ggaaaacctg cagatcatca gaggaaatat gtactacgaa
     1801 aattcctatg ccttagcagt cttatctaac tatgatgcaa ataaaaccgg actgaaggag
     1861 ctgcccatga gaaatttaca ggaaatcctg catggcgccg tgcggttcag caacaaccct
     1921 gccctgtgca acgtggagag catccagtgg cgggacatag tcagcagtga ctttctcagc
     1981 aacatgtcga tggacttcca gaaccacctg ggcagctgcc aaaagtgtga tccaagctgt
     2041 cccaatggga gctgctgggg tgcaggagag gagaactgcc agaaactgac caaaatcatc
     2101 tgtgcccagc agtgctccgg gcgctgccgt ggcaagtccc ccagtgactg ctgccacaac
     2161 cagtgtgctg caggctgcac aggcccccgg gagagcgact gcctggtctg ccgcaaattc
     2221 cgagacgaag ccacgtgcaa ggacacctgc cccccactca tgctctacaa ccccaccacg
     2281 taccagatgg atgtgaaccc cgagggcaaa tacagctttg gtgccacctg cgtgaagaag
     2341 tgtccccgta attatgtggt gacagatcac ggctcgtgcg tccgagcctg tggggccgac
     2401 agctatgaga tggaggaaga cggcgtccgc aagtgtaaga agtgcgaagg gccttgccgc
     2461 aaagtgtgta acggaatagg tattggtgaa tttaaagact cactctccat aaatgctacg
     2521 aatattaaac acttcaaaaa ctgcacctcc atcagtggcg atctccacat cctgccggtg
     2581 gcatttaggg gtgactcctt cacacatact cctcctctgg atccacagga actggatatt
     2641 ctgaaaaccg taaaggaaat cacagggttt ttgctgattc aggcttggcc tgaaaacagg
     2701 acggacctcc atgcctttga gaacctagaa atcatacgcg gcaggaccaa gcaacatggt
     2761 cagttttctc ttgcagtcgt cagcctgaac ataacatcct tgggattacg ctccctcaag
     2821 gagataagtg atggagatgt gataatttca ggaaacaaaa atttgtgcta tgcaaataca
     2881 ataaactgga aaaaactgtt tgggacctcc ggtcagaaaa ccaaaattat aagcaacaga
     2941 ggtgaaaaca gctgcaaggc cacaggccag gtctgccatg ccttgtgctc ccccgagggc
     3001 tgctggggcc cggagcccag ggactgcgtc tcttgccgga atgtcagccg aggcagggaa
     3061 tgcgtggaca agtgcaacct tctggagggt gagccaaggg agtttgtgga gaactctgag
     3121 tgcatacagt gccacccaga gtgcctgcct caggccatga acatcacctg cacaggacgg
     3181 ggaccagaca actgtatcca gtgtgcccac tacattgacg gcccccactg cgtcaagacc
     3241 tgcccggcag gagtcatggg agaaaacaac accctggtct ggaagtacgc agacgccggc
     3301 catgtgtgcc acctgtgcca tccaaactgc acctacggat gcactgggcc aggtcttgaa
     3361 ggctgtccaa cgaatgggcc taagatcccg tccatcgcca ctgggatggt gggggccctc
     3421 ctcttgctgc tggtggtggc cctggggatc ggcctcttca tgcgaaggcg ccacatcgtt
     3481 cggaagcgca cgctgcggag gctgctgcag gagagggagc ttgtggagcc tcttacaccc
     3541 agtggagaag ctcccaacca agctctcttg aggatcttga aggaaactga attcaaaaag
     3601 atcaaagtgc tgggctccgg tgcgttcggc acggtgtata agggactctg gatcccagaa
     3661 ggtgagaaag ttaaaattcc cgtcgctatc aaggaattaa gagaagcaac atctccgaaa
     3721 gccaacaagg aaatcctcga tgaagcctac gtgatggcca gcgtggacaa cccccacgtg
     3781 tgccgcctgc tgggcatctg cctcacctcc accgtgcagc tcatcacgca gctcatgccc
     3841 ttcggctgcc tcctggacta tgtccgggaa cacaaagaca atattggctc ccagtacctg
     3901 ctcaactggt gtgtgcagat cgcaaagggc atgaactact tggaggaccg tcgcttggtg
     3961 caccgcgacc tggcagccag gaacgtactg gtgaaaacac tgcagcatgt caagatcaca
     4021 gattttgggc tggccaaact gctgggtgcg gaagagaaag aataccatgc agaaggaggc
     4081 aaagtgccta tcaagtggat ggcattggaa tcaattttac acagaatcta tacccaccag
     4141 agtgatgtct ggagctacgg ggtgaccgtt tgggagttga tgacctttgg atccaagcca
     4201 tatgacggaa tccctgccag cgagatctcc tccatcctgg agaaaggaga acgcctccct
     4261 cagccaccca tatgtaccat cgatgtctac atgatcatgg tcaagtgctg gatgatagac
     4321 gcagatagtc gcccaaagtt ccgtgagttg atcatcgaat tctccaaaat ggcccgagac
     4381 ccccagcgct accttgtcat tcagggggat gaaagaatgc atttgccaag tcctacagac
     4441 tccaacttct accgtgccct gatggatgaa gaagacatgg acgacgtggt ggatgccgac
     4501 gagtacctca tcccacagca gggcttcttc agcagcccct ccacgtcacg gactcccctc
     4561 ctgagctctc tgagtgcaac cagcaacaat tccaccgtgg cttgcattga tagaaatggg
     4621 ctgcaaagct gtcccatcaa ggaagacagc ttcttgcagc gatacagctc agaccccaca
     4681 ggcgccttga ctgaggacag catagacgac accttcctcc cagtgcctga atacataaac
     4741 cagtccgttc ccaaaaggcc cgctggctct gtgcagaatc ctgtctatca caatcagcct
     4801 ctgaaccccg cgcccagcag agacccacac taccaggacc cccacagcac tgcagtgggc
     4861 aaccccgagt atctcaacac tgtccagccc acctgtgtca acagcacatt cgacagccct
     4921 gcccactggg cccagaaagg cagccaccaa attagcctgg acaaccctga ctaccagcag
     4981 gacttctttc ccaaggaagc caagccaaat ggcatcttta agggctccac agctgaaaat
     5041 gcagaatacc taagggtcgc gccacaaagc agtgaattta ttggagcata gaacccagct
     5101 ttcttgtaca aagtggtgat atccaattaa ccctcactaa aggtaaagag tgatgtaaat
     5161 agaagttccc tatagtgagt cgtattacca cccaactttt ctatacaaag tggttgatat
     5221 ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc
     5281 taaccctctc ctcggtctcg attctacgcg taccggttag taatgagatc cctccccccc
     5341 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     5401 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     5461 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     5521 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     5581 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     5641 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     5701 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     5761 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     5821 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     5881 aaaaacacga tgataatatg gccacacata tgaccgagta caagcccacg gtgcgcctcg
     5941 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     6001 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     6061 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     6121 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     6181 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     6241 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     6301 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     6361 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     6421 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     6481 ggtgcatgac ccgcaagccc ggtgcctaaa tcgatagatc ctaatcaacc tctggattac
     6541 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6601 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6661 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6721 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6781 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6841 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6901 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6961 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7021 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7081 agtcggatct ccctttgggc cgcctccccg cctgagatcc tttaagacca atgacttaca
     7141 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     7201 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     7261 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7321 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7381 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     7441 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg cccgggttaa
     7501 ttaaggaaag ggctagatca ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     7561 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     7621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7741 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7801 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     7861 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     7921 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     7981 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8041 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8101 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8161 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8221 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8281 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8341 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8401 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8461 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8521 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8581 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8701 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8761 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     8821 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     8881 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     8941 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9001 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9061 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9121 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9181 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9241 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9301 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9361 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     9421 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     9481 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     9541 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcaagct catggctgac
     9601 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9661 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg
     9721 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     9781 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     9841 ggataacaat ttcacacagg aaacagctat gacatgatta cgaatttcac aaataaagca
     9901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9961 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    10021 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
    10081 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
    10141 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
    10201 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
    10261 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
    10321 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
    10381 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
    10441 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
    10501 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
    10561 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
    10621 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
    10681 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
    10741 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
    10801 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
    10861 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
    10921 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
    10981 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
    11041 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
    11101 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
    11161 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
    11221 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
    11281 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
    11341 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    11401 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    11461 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    11521 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    11581 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    11641 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    11701 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    11761 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    11821 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    11881 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    11941 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    12001 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    12061 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    12121 ag
//
