LOCUS       sequence-250669-       10711 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3R1 N564D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3R1-N564D
FEATURES             Location/Qualifiers
     source          1..10711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3451..3477
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5648..5672)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5725..5766
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5787..6354
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5804..6356
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5948..5965)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6175..6194
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6368..6370,6371..6373,6374..7087)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6368..6370,6371..6373,6374..7084)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6413..6434)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6674..6693)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7021..7042
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7101..7689
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7154..7174)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7572..7583)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7764..7997
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8116..8134)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8202..8306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8307..8375,8376..9167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8525..8544)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9338..9926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9827..9846
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10080..10097
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10337..10358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10373..10378,10379..10396,10397..10403)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10411..10427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10416..10438
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10435..10451
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10435..10451
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10501..10520
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatggtt tacccatacg atgttccaga ttacgctagt
     3481 gctgaggggt accagtacag agcgctgtat gattataaaa aggaaagaga agaagatatt
     3541 gacttgcact tgggtgacat attgactgtg aataaagggt ccttagtagc tcttggattc
     3601 agtgatggac aggaagccag gcctgaagaa attggctggt taaatggcta taatgaaacc
     3661 acaggggaaa ggggggactt tccgggaact tatgtagaat atattggaag gaaaaaaatc
     3721 tcgcctccca caccaaagcc ccggccacct cggcctcttc ctgttgcacc aggttcttcg
     3781 aaaactgaag cagatgttga acaacaagct ttgactctcc cggatcttgc agagcagttt
     3841 gcccctcctg acattgcccc gcctcttctt atcaagctcg tggaagccat tgaaaagaaa
     3901 ggtctggaat gttcaactct atacagaaca cagagctcca gcaacctggc agaattacga
     3961 cagcttcttg attgtgatac accctccgtg gacttggaaa tgatcgatgt gcacgttttg
     4021 gctgacgctt tcaaacgcta tctcctggac ttaccaaatc ctgtcattcc agcagccgtt
     4081 tacagtgaaa tgatttcttt agctccagaa gtacaaagct ccgaagaata tattcagcta
     4141 ttgaagaagc ttattaggtc gcctagcata cctcatcagt attggcttac gcttcagtat
     4201 ttgttaaaac atttcttcaa gctctctcaa acctccagca aaaatctgtt gaatgcaaga
     4261 gtactctctg aaattttcag ccctatgctt ttcagattct cagcagccag ctctgataat
     4321 actgaaaacc tcataaaagt tatagaaatt ttaatctcaa ctgaatggaa tgaacgacag
     4381 cctgcaccag cactgcctcc taaaccacca aaacctacta ctgtagccaa caacggtatg
     4441 aataacaata tgtccttaca agatgctgaa tggtactggg gagatatctc gagggaagaa
     4501 gtgaatgaaa aacttcgaga tacagcagac gggacctttt tggtacgaga tgcgtctact
     4561 aaaatgcatg gtgattatac tcttacacta aggaaagggg gaaataacaa attaatcaaa
     4621 atatttcatc gagatgggaa atatggcttc tctgacccat taaccttcag ttctgtggtt
     4681 gaattaataa accactaccg gaatgaatct ctagctcagt ataatcccaa attggatgtg
     4741 aaattacttt atccagtatc caaataccaa caggatcaag ttgtcaaaga agataatatt
     4801 gaagctgtag ggaaaaaatt acatgaatat aacactcagt ttcaagaaaa aagtcgagaa
     4861 tatgatagat tatatgaaga atatacccgc acatcccagg aaatccaaat gaaaaggaca
     4921 gctattgaag catttaatga aaccataaaa atatttgaag aacagtgcca gacccaagag
     4981 cggtacagca aagaatacat agaaaagttt aaacgtgaag gcaatgagaa agaaatacaa
     5041 aggattatgc ataattatga taagttgaag tctcgaatca gtgaaattat tgacagtaga
     5101 agaagattgg aagaagactt gaagaagcag gcagctgagt atcgagaaat tgacaaacgt
     5161 atggacagca ttaaaccaga ccttatccag ctgagaaaga cgagagacca atacttgatg
     5221 tggttgactc aaaaaggtgt tcggcaaaag aagttgaacg agtggttggg caatgaaaac
     5281 actgaagacc aatattcact ggtggaagat gatgaagatt tgccccatca tgatgagaag
     5341 acatggaatg ttggaagcag caaccgaaac aaagctgaaa acctgttgcg agggaagcga
     5401 gatggcactt ttcttgtccg ggagagcagt aaacagggct gctatgcctg ctctgtagtg
     5461 gtggacggcg aagtaaagca ttgtgtcata aacaaaacag caactggcta tggctttgcc
     5521 gagccctata acttgtacag ctctctgaaa gaactggtgc tacattacca acacacctcc
     5581 cttgtgcagc acaacgactc cctcaatgtc acactagcct acccagtata tgcacagcag
     5641 aggcgatgcc caactttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg
     5701 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     5761 tctacgcgta ccggttagta atgagatccc tccccccccc ctaacgttac tggccgaagc
     5821 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     5881 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     5941 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     6001 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     6061 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     6121 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     6181 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     6241 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     6301 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     6361 cacacatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     6421 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     6481 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     6541 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     6601 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     6661 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     6721 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     6781 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     6841 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     6901 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     6961 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     7021 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     7081 caagtaaatc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     7141 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     7201 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7261 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7321 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7381 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7441 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     7501 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7561 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7621 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7681 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7741 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7801 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7861 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7921 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7981 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     8041 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     8101 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     8161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8521 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     8581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9961 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10021 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10081 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10141 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
    10201 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10261 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    10321 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10381 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10441 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    10501 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10561 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    10621 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10681 tttgttaaat atgtactaca aacttagtag t
//
