LOCUS       sequence-250673-       10696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3R1 Q455_K459delQFQEK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3R1-Q455_K459delQFQEK
FEATURES             Location/Qualifiers
     source          1..10696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3451..3477
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5633..5657)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5710..5751
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5772..6339
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5789..6341
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5933..5950)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6160..6179
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6353..6355,6356..6358,6359..7072)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6353..6355,6356..6358,6359..7069)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6398..6419)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6659..6678)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7006..7027
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7086..7674
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7139..7159)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7557..7568)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7749..7982
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8101..8119)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8187..8291
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8292..8360,8361..9152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8510..8529)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9323..9911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9812..9831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10065..10082
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10322..10343
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10358..10363,10364..10381,10382..10388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10396..10412
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10401..10423
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10420..10436
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10420..10436
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10486..10505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagt tggcatggtt tacccatacg atgttccaga ttacgctagt
     3481 gctgaggggt accagtacag agcgctgtat gattataaaa aggaaagaga agaagatatt
     3541 gacttgcact tgggtgacat attgactgtg aataaagggt ccttagtagc tcttggattc
     3601 agtgatggac aggaagccag gcctgaagaa attggctggt taaatggcta taatgaaacc
     3661 acaggggaaa ggggggactt tccgggaact tatgtagaat atattggaag gaaaaaaatc
     3721 tcgcctccca caccaaagcc ccggccacct cggcctcttc ctgttgcacc aggttcttcg
     3781 aaaactgaag cagatgttga acaacaagct ttgactctcc cggatcttgc agagcagttt
     3841 gcccctcctg acattgcccc gcctcttctt atcaagctcg tggaagccat tgaaaagaaa
     3901 ggtctggaat gttcaactct atacagaaca cagagctcca gcaacctggc agaattacga
     3961 cagcttcttg attgtgatac accctccgtg gacttggaaa tgatcgatgt gcacgttttg
     4021 gctgacgctt tcaaacgcta tctcctggac ttaccaaatc ctgtcattcc agcagccgtt
     4081 tacagtgaaa tgatttcttt agctccagaa gtacaaagct ccgaagaata tattcagcta
     4141 ttgaagaagc ttattaggtc gcctagcata cctcatcagt attggcttac gcttcagtat
     4201 ttgttaaaac atttcttcaa gctctctcaa acctccagca aaaatctgtt gaatgcaaga
     4261 gtactctctg aaattttcag ccctatgctt ttcagattct cagcagccag ctctgataat
     4321 actgaaaacc tcataaaagt tatagaaatt ttaatctcaa ctgaatggaa tgaacgacag
     4381 cctgcaccag cactgcctcc taaaccacca aaacctacta ctgtagccaa caacggtatg
     4441 aataacaata tgtccttaca agatgctgaa tggtactggg gagatatctc gagggaagaa
     4501 gtgaatgaaa aacttcgaga tacagcagac gggacctttt tggtacgaga tgcgtctact
     4561 aaaatgcatg gtgattatac tcttacacta aggaaagggg gaaataacaa attaatcaaa
     4621 atatttcatc gagatgggaa atatggcttc tctgacccat taaccttcag ttctgtggtt
     4681 gaattaataa accactaccg gaatgaatct ctagctcagt ataatcccaa attggatgtg
     4741 aaattacttt atccagtatc caaataccaa caggatcaag ttgtcaaaga agataatatt
     4801 gaagctgtag ggaaaaaatt acatgaatat aacactagtc gagaatatga tagattatat
     4861 gaagaatata cccgcacatc ccaggaaatc caaatgaaaa ggacagctat tgaagcattt
     4921 aatgaaacca taaaaatatt tgaagaacag tgccagaccc aagagcggta cagcaaagaa
     4981 tacatagaaa agtttaaacg tgaaggcaat gagaaagaaa tacaaaggat tatgcataat
     5041 tatgataagt tgaagtctcg aatcagtgaa attattgaca gtagaagaag attggaagaa
     5101 gacttgaaga agcaggcagc tgagtatcga gaaattgaca aacgtatgaa cagcattaaa
     5161 ccagacctta tccagctgag aaagacgaga gaccaatact tgatgtggtt gactcaaaaa
     5221 ggtgttcggc aaaagaagtt gaacgagtgg ttgggcaatg aaaacactga agaccaatat
     5281 tcactggtgg aagatgatga agatttgccc catcatgatg agaagacatg gaatgttgga
     5341 agcagcaacc gaaacaaagc tgaaaacctg ttgcgaggga agcgagatgg cacttttctt
     5401 gtccgggaga gcagtaaaca gggctgctat gcctgctctg tagtggtgga cggcgaagta
     5461 aagcattgtg tcataaacaa aacagcaact ggctatggct ttgccgagcc ctataacttg
     5521 tacagctctc tgaaagaact ggtgctacat taccaacaca cctcccttgt gcagcacaac
     5581 gactccctca atgtcacact agcctaccca gtatatgcac agcagaggcg atgcccaact
     5641 ttcttgtaca aagtggttga tatccagcac agtggcggcc gctcgagtct agagggcccg
     5701 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     5761 tagtaatgag atccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     5821 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     5881 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     5941 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     6001 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     6061 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     6121 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     6181 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     6241 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     6301 cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac atatggtgag
     6361 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     6421 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     6481 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     6541 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     6601 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     6661 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     6721 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     6781 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     6841 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     6901 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     6961 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     7021 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aaatcgatag
     7081 atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7141 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7201 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7261 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7321 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7381 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7441 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     7501 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7561 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7621 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga
     7681 tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7741 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
     7801 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7861 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7921 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7981 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     8041 agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg
     8101 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     8161 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     8221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     8281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     8341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     8401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     8461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     8521 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     8581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     8641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     9001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     9061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     9121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     9181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     9241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     9301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     9361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     9421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     9481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     9541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     9601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    10021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    10081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10141 aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    10201 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    10261 aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10321 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10381 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga
    10441 ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10501 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
    10561 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
    10621 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
    10681 ctacaaactt agtagt
//
