LOCUS       sequence-250676-       10718 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PIK3R1 R348*.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PIK3R1-R348*
FEATURES             Location/Qualifiers
     source          1..10718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5627..5653
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5655..5679)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5732..5773
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5794..6361
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5811..6363
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5955..5972)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6182..6201
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6375..6377,6378..6380,6381..7094)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6375..6377,6378..6380,6381..7091)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6420..6441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6681..6700)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7028..7049
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7108..7696
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7161..7181)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7579..7590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7771..8004
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8123..8141)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8209..8313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8314..8382,8383..9174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8532..8551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9345..9933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9834..9853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10087..10104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10344..10365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10380..10385,10386..10403,10404..10410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10418..10434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10423..10445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10442..10458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10442..10458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10508..10527
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggctctcga gatggatagt gctgaggggt accagtacag
     3481 agcgctgtat gattataaaa aggaaagaga agaagatatt gacttgcact tgggtgacat
     3541 attgactgtg aataaagggt ccttagtagc tcttggattc agtgatggac aggaagccag
     3601 gcctgaagaa attggctggt taaatggcta taatgaaacc acaggggaaa ggggggactt
     3661 tccgggaact tatgtagaat atattggaag gaaaaaaatc tcgcctccca caccaaagcc
     3721 ccggccacct cggcctcttc ctgttgcacc aggttcttcg aaaactgaag cagatgttga
     3781 acaacaagct ttgactctcc cggatcttgc agagcagttt gcccctcctg acattgcccc
     3841 gcctcttctt atcaagctcg tggaagccat tgaaaagaaa ggtctggaat gttcaactct
     3901 atacagaaca cagagctcca gcaacctggc agaattacga cagcttcttg attgtgatac
     3961 accctccgtg gacttggaaa tgatcgatgt gcacgttttg gctgacgctt tcaaacgcta
     4021 tctcctggac ttaccaaatc ctgtcattcc agcagccgtt tacagtgaaa tgatttcttt
     4081 agctccagaa gtacaaagct ccgaagaata tattcagcta ttgaagaagc ttattaggtc
     4141 gcctagcata cctcatcagt attggcttac gcttcagtat ttgttaaaac atttcttcaa
     4201 gctctctcaa acctccagca aaaatctgtt gaatgcaaga gtactctctg aaattttcag
     4261 ccctatgctt ttcagattct cagcagccag ctctgataat actgaaaacc tcataaaagt
     4321 tatagaaatt ttaatctcaa ctgaatggaa tgaacgacag cctgcaccag cactgcctcc
     4381 taaaccacca aaacctacta ctgtagccaa caacggtatg aataacaata tgtccttaca
     4441 agatgctgaa tggtactggg gagatatctc gagggaagaa gtgaatgaaa aactttgaga
     4501 tacagcagac gggacctttt tggtacgaga tgcgtctact aaaatgcatg gtgattatac
     4561 tcttacacta aggaaagggg gaaataacaa attaatcaaa atatttcatc gagatgggaa
     4621 atatggcttc tctgacccat taaccttcag ttctgtggtt gaattaataa accactaccg
     4681 gaatgaatct ctagctcagt ataatcccaa attggatgtg aaattacttt atccagtatc
     4741 caaataccaa caggatcaag ttgtcaaaga agataatatt gaagctgtag ggaaaaaatt
     4801 acatgaatat aacactcagt ttcaagaaaa aagtcgagaa tatgatagat tatatgaaga
     4861 atatacccgc acatcccagg aaatccaaat gaaaaggaca gctattgaag catttaatga
     4921 aaccataaaa atatttgaag aacagtgcca gacccaagag cggtacagca aagaatacat
     4981 agaaaagttt aaacgtgaag gcaatgagaa agaaatacaa aggattatgc ataattatga
     5041 taagttgaag tctcgaatca gtgaaattat tgacagtaga agaagattgg aagaagactt
     5101 gaagaagcag gcagctgagt atcgagaaat tgacaaacgt atgaacagca ttaaaccaga
     5161 ccttatccag ctgagaaaga cgagagacca atacttgatg tggttgactc aaaaaggtgt
     5221 tcggcaaaag aagttgaacg agtggttggg caatgaaaac actgaagacc aatattcact
     5281 ggtggaagat gatgaagatt tgccccatca tgatgagaag acatggaatg ttggaagcag
     5341 caaccgaaac aaagctgaaa acctgttgcg agggaagcga gatggcactt ttcttgtccg
     5401 ggagagcagt aaacagggct gctatgcctg ctctgtagtg gtggacggcg aagtaaagca
     5461 ttgtgtcata aacaaaacag caactggcta tggctttgcc gagccctata acttgtacag
     5521 ctctctgaaa gaactggtgc tacattacca acacacctcc cttgtgcagc acaacgactc
     5581 cctcaatgtc acactagcct acccagtata tgcacagcag aggcgatacc catacgatgt
     5641 tccagattac gctgacccag ctttcttgta caaagtggtt gatatccagc acagtggcgg
     5701 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     5761 tctcgattct acgcgtaccg gttagtaatg agatccctcc ccccccccta acgttactgg
     5821 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     5881 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     5941 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     6001 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     6061 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     6121 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     6181 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     6241 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     6301 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     6361 atatggccac acatatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     6421 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     6481 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     6541 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     6601 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     6661 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     6721 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     6781 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     6841 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     6901 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     6961 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     7021 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     7081 agctgtacaa gtaaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     7141 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7201 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7261 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7321 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7381 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7441 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7501 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7561 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7621 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7681 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     7741 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7801 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7861 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7921 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7981 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     8041 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     8101 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8161 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8221 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     8281 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     8341 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8401 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8461 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8521 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     8581 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8641 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8701 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8761 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8821 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8881 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8941 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9001 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9061 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9121 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9181 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9241 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     9301 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9361 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9421 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9481 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9541 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9601 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9661 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9721 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9781 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9841 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9901 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9961 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10021 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10081 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10141 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
    10201 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    10261 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
    10321 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10381 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    10441 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
    10501 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    10561 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    10621 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10681 aattgtattt gttaaatatg tactacaaac ttagtagt
//
