LOCUS       sequence-250683-       12266 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ERBB2 V777_G778insGSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12266)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB2-V777_G778insGSP
FEATURES             Location/Qualifiers
     source          1..12266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7226..7250)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7258..7276
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7301..7320)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7302..7320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7326..7346)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7400..7441
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7462..8029
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7479..8031
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7623..7640)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7850..7869
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8043..8642
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8043..8062)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8539..8559
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8656..9244
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8709..8729)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9127..9138)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9319..9552
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9671..9689)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9757..9861
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9862..9930,9931..10722)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10080..10099)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10893..11481
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11382..11401
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11635..11652
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11892..11913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11928..11933,11934..11951,11952..11958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11966..11982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11971..11993
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11990..12006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11990..12006
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12056..12075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gagctggcgg ccttgtgccg ctgggggctc
     3481 ctcctcgccc tcttgccccc cggagccgcg agcacccaag tgtgcaccgg cacagacatg
     3541 aagctgcggc tccctgccag tcccgagacc cacctggaca tgctccgcca cctctaccag
     3601 ggctgccagg tggtgcaggg aaacctggaa ctcacctacc tgcccaccaa tgccagcctg
     3661 tccttcctgc aggatatcca ggaggtgcag ggctacgtgc tcatcgctca caaccaagtg
     3721 aggcaggtcc cactgcagag gctgcggatt gtgcgaggca cccagctctt tgaggacaac
     3781 tatgccctgg ccgtgctaga caatggagac ccgctgaaca ataccacccc tgtcacaggg
     3841 gcctccccag gaggcctgcg ggagctgcag cttcgaagcc tcacagagat cttgaaagga
     3901 ggggtcttga tccagcggaa cccccagctc tgctaccagg acacgatttt gtggaaggac
     3961 atcttccaca agaacaacca gctggctctc acactgatag acaccaaccg ctctcgggcc
     4021 tgccacccct gttctccgat gtgtaagggc tcccgctgct ggggagagag ttctgaggat
     4081 tgtcagagcc tgacgcgcac tgtctgtgcc ggtggctgtg cccgctgcaa ggggccactg
     4141 cccactgact gctgccatga gcagtgtgct gccggctgca cgggccccaa gcactctgac
     4201 tgcctggcct gcctccactt caaccacagt ggcatctgtg agctgcactg cccagccctg
     4261 gtcacctaca acacagacac gtttgagtcc atgcccaatc ccgagggccg gtatacattc
     4321 ggcgccagct gtgtgactgc ctgtccctac aactaccttt ctacggacgt gggatcctgc
     4381 accctcgtct gccccctgca caaccaagag gtgacagcag aggatggaac acagcggtgt
     4441 gagaagtgca gcaagccctg tgcccgagtg tgctatggtc tgggcatgga gcacttgcga
     4501 gaggtgaggg cagttaccag tgccaatatc caggagtttg ctggctgcaa gaagatcttt
     4561 gggagcctgg catttctgcc ggagagcttt gatggggacc cagcctccaa cactgccccg
     4621 ctccagccag agcagctcca agtgtttgag actctggaag agatcacagg ttacctatac
     4681 atctcagcat ggccggacag cctgcctgac ctcagcgtct tccagaacct gcaagtaatc
     4741 cggggacgaa ttctgcacaa tggcgcctac tcgctgaccc tgcaagggct gggcatcagc
     4801 tggctggggc tgcgctcact gagggaactg ggcagtggac tggccctcat ccaccataac
     4861 acccacctct gcttcgtgca cacggtgccc tgggaccagc tctttcggaa cccgcaccaa
     4921 gctctgctcc acactgccaa ccggccagag gacgagtgtg tgggcgaggg cctggcctgc
     4981 caccagctgt gcgcccgagg gcactgctgg ggtccagggc ccacccagtg tgtcaactgc
     5041 agccagttcc ttcggggcca ggagtgcgtg gaggaatgcc gagtactgca ggggctcccc
     5101 agggagtatg tgaatgccag gcactgtttg ccgtgccacc ctgagtgtca gccccagaat
     5161 ggctcagtga cctgttttgg accggaggct gaccagtgtg tggcctgtgc ccactataag
     5221 gaccctccct tctgcgtggc ccgctgcccc agcggtgtga aacctgacct ctcctacatg
     5281 cccatctgga agtttccaga tgaggagggc gcatgccagc cttgccccat caactgcacc
     5341 cactcctgtg tggacctgga tgacaagggc tgccccgccg agcagagagc cagccctctg
     5401 acgtccatca tctctgcggt ggttggcatt ctgctggtcg tggtcttggg ggtggtcttt
     5461 gggatcctca tcaagcgacg gcagcagaag atccggaagt acacgatgcg gagactgctg
     5521 caggaaacgg agctggtgga gccgctgaca cctagcggag cgatgcccaa ccaggcgcag
     5581 atgcggatcc tgaaagagac ggagctgagg aaggtgaagg tgcttggatc tggcgctttt
     5641 ggcacagtct acaagggcat ctggatccct gatggggaga atgtgaaaat tccagtggcc
     5701 atcaaagtgt tgagggaaaa cacatccccc aaagccaaca aagaaatctt agacgaagca
     5761 tacgtgatgg ctggtgtggg ctccccgggc tccccatatg tctcccgcct tctgggcatc
     5821 tgcctgacat ccacggtgca gctggtgaca cagcttatgc cctatggctg cctcttagac
     5881 catgtccggg aaaaccgcgg acgcctgggc tcccaggacc tgctgaactg gtgtatgcag
     5941 attgccaagg ggatgagcta cctggaggat gtgcggctcg tacacaggga cttggccgct
     6001 cggaacgtgc tggtcaagag tcccaaccat gtcaaaatta cagacttcgg gctggctcgg
     6061 ctgctggaca ttgacgagac agagtaccat gcagatgggg gcaaggtgcc catcaagtgg
     6121 atggcgctgg agtccattct ccgccggcgg ttcacccacc agagtgatgt gtggagttat
     6181 ggtgtgactg tgtgggagct gatgactttt ggggccaaac cttacgatgg gatcccagcc
     6241 cgggagatcc ctgacctgct ggaaaagggg gagcggctgc cccagccccc catctgcacc
     6301 attgatgtct acatgatcat ggtcaaatgt tggatgattg actctgaatg tcggccaaga
     6361 ttccgggagt tggtgtctga attctcccgc atggccaggg acccccagcg ctttgtggtc
     6421 atccagaatg aggacttggg cccagccagt cccttggaca gcaccttcta ccgctcactg
     6481 ctggaggacg atgacatggg ggacctggtg gatgctgagg agtatctggt accccagcag
     6541 ggcttcttct gtccagaccc tgccccgggc gctgggggca tggtccacca caggcaccgc
     6601 agctcatcta ccaggagtgg cggtggggac ctgacactag ggctggagcc ctctgaagag
     6661 gaggccccca ggtctccact ggcaccctcc gaaggggctg gctccgatgt atttgatggt
     6721 gacctgggaa tgggggcagc caaggggctg caaagcctcc ccacacatga ccccagccct
     6781 ctacagcggt acagtgagga ccccacagta cccctgccct ctgagactga tggctacgtt
     6841 gcccccctga cctgcagccc ccagcctgaa tatgtgaacc agccagatgt tcggccccag
     6901 cccccttcgc cccgagaggg ccctctgcct gctgcccgac ctgctggtgc cactctggaa
     6961 aggcccaaga ctctctcccc agggaagaat ggggtcgtca aagacgtttt tgcctttggg
     7021 ggtgccgtgg agaaccccga gtacttgaca ccccagggag gagctgcccc tcagccccac
     7081 cctcctcctg ccttcagccc agccttcgac aacctctatt actgggacca ggacccacca
     7141 gagcgggggg ctccacccag caccttcaaa gggacaccta cggcagagaa cccagagtac
     7201 ctgggtctgg acgtgccagt gtagaaccca gctttcttgt acaaagtggt gatatccaat
     7261 taaccctcac taaagggatt agtatttagt agtaatagag ccctatagtg agtcgtatta
     7321 ccaccccact tttctataca aagtggttga tatccagcac agtggcggcc gctcgagtct
     7381 agagggcccg cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     7441 gcgtaccggt tagtaatgag atccctcccc cccccctaac gttactggcc gaagccgctt
     7501 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
     7561 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
     7621 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
     7681 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
     7741 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
     7801 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
     7861 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
     7921 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
     7981 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac
     8041 atatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     8101 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     8161 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     8221 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     8281 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     8341 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     8401 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     8461 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     8521 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     8581 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     8641 aaatcgatag atcctaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     8701 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     8761 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     8821 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     8881 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     8941 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     9001 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     9061 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     9121 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     9181 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     9241 ccgcctgaga tcctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     9301 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
     9361 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     9421 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     9481 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     9541 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     9601 aatgaatatc agagagtgag aggcccgggt taattaagga aagggctaga tcattcttga
     9661 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     9721 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     9781 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     9841 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10081 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
    10141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    10741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    10801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    10861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    11641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    11701 ccgattcatt aatgcagcaa gctcatggct gactaatttt ttttatttat gcagaggccg
    11761 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    11821 gcttttgcaa aaagctcccc gtggcacgac aggtttcccg actggaaagc gggcagtgag
    11881 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
    11941 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
    12001 tatgacatga ttacgaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    12061 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    12121 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    12181 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    12241 taaatatgta ctacaaactt agtagt
//
