LOCUS       sequence-250687-       12122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR R108K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12122)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-R108K
FEATURES             Location/Qualifiers
     source          1..12122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    155..272
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        320..1498
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          551..1489
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1446..1466
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1518..1542
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1545..1616,1617..3479,3480..3548,3549..5177)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIKGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5179..5203)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5211..5229
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5254..5273)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5255..5273)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5279..5299)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5353..5394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5415..5982
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5432..5984
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5576..5593)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5803..5822
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5996..6595
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5996..6015)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6492..6512
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6609..7197
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6662..6682)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7080..7091)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7272..7505
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7624..7642)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7710..7814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7815..7883,7884..8675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8846..9434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9335..9354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9588..9605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9845..9866
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9881..9886,9887..9904,9905..9911)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9919..9935
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9924..9946
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9943..9959
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9943..9959
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10009..10028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10220..10853
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10900..11025
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11522..11755
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11940..11984
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
       61 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
      121 cgccgaattc acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
      181 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
      241 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
      301 cagtttggac tagtcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
      361 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
      421 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
      481 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
      541 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
      601 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
      661 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
      721 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
      781 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
      841 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
      901 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
      961 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     1021 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     1081 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     1141 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     1201 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     1261 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     1321 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     1381 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     1441 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaag
     1501 cggccctgca gatatcaaca agtttgtaca aaaaagcagg caccatgcga ccctccggga
     1561 cggccggggc agcgctcctg gcgctgctgg ctgcgctctg cccggcgagt cgggctctgg
     1621 aggaaaagaa agtttgccaa ggcacgagta acaagctcac gcagttgggc acttttgaag
     1681 atcattttct cagcctccag aggatgttca ataactgtga ggtggtcctt gggaatttgg
     1741 aaattaccta tgtgcagagg aattatgatc tttccttctt aaagaccatc caggaggtgg
     1801 ctggttatgt cctcattgcc ctcaacacag tggagcgaat tcctttggaa aacctgcaga
     1861 tcatcaaagg aaatatgtac tacgaaaatt cctatgcctt agcagtctta tctaactatg
     1921 atgcaaataa aaccggactg aaggagctgc ccatgagaaa tttacaggaa atcctgcatg
     1981 gcgccgtgcg gttcagcaac aaccctgccc tgtgcaacgt ggagagcatc cagtggcggg
     2041 acatagtcag cagtgacttt ctcagcaaca tgtcgatgga cttccagaac cacctgggca
     2101 gctgccaaaa gtgtgatcca agctgtccca atgggagctg ctggggtgca ggagaggaga
     2161 actgccagaa actgaccaaa atcatctgtg cccagcagtg ctccgggcgc tgccgtggca
     2221 agtcccccag tgactgctgc cacaaccagt gtgctgcagg ctgcacaggc ccccgggaga
     2281 gcgactgcct ggtctgccgc aaattccgag acgaagccac gtgcaaggac acctgccccc
     2341 cactcatgct ctacaacccc accacgtacc agatggatgt gaaccccgag ggcaaataca
     2401 gctttggtgc cacctgcgtg aagaagtgtc cccgtaatta tgtggtgaca gatcacggct
     2461 cgtgcgtccg agcctgtggg gccgacagct atgagatgga ggaagacggc gtccgcaagt
     2521 gtaagaagtg cgaagggcct tgccgcaaag tgtgtaacgg aataggtatt ggtgaattta
     2581 aagactcact ctccataaat gctacgaata ttaaacactt caaaaactgc acctccatca
     2641 gtggcgatct ccacatcctg ccggtggcat ttaggggtga ctccttcaca catactcctc
     2701 ctctggatcc acaggaactg gatattctga aaaccgtaaa ggaaatcaca gggtttttgc
     2761 tgattcaggc ttggcctgaa aacaggacgg acctccatgc ctttgagaac ctagaaatca
     2821 tacgcggcag gaccaagcaa catggtcagt tttctcttgc agtcgtcagc ctgaacataa
     2881 catccttggg attacgctcc ctcaaggaga taagtgatgg agatgtgata atttcaggaa
     2941 acaaaaattt gtgctatgca aatacaataa actggaaaaa actgtttggg acctccggtc
     3001 agaaaaccaa aattataagc aacagaggtg aaaacagctg caaggccaca ggccaggtct
     3061 gccatgcctt gtgctccccc gagggctgct ggggcccgga gcccagggac tgcgtctctt
     3121 gccggaatgt cagccgaggc agggaatgcg tggacaagtg caaccttctg gagggtgagc
     3181 caagggagtt tgtggagaac tctgagtgca tacagtgcca cccagagtgc ctgcctcagg
     3241 ccatgaacat cacctgcaca ggacggggac cagacaactg tatccagtgt gcccactaca
     3301 ttgacggccc ccactgcgtc aagacctgcc cggcaggagt catgggagaa aacaacaccc
     3361 tggtctggaa gtacgcagac gccggccatg tgtgccacct gtgccatcca aactgcacct
     3421 acggatgcac tgggccaggt cttgaaggct gtccaacgaa tgggcctaag atcccgtcca
     3481 tcgccactgg gatggtgggg gccctcctct tgctgctggt ggtggccctg gggatcggcc
     3541 tcttcatgcg aaggcgccac atcgttcgga agcgcacgct gcggaggctg ctgcaggaga
     3601 gggagcttgt ggagcctctt acacccagtg gagaagctcc caaccaagct ctcttgagga
     3661 tcttgaagga aactgaattc aaaaagatca aagtgctggg ctccggtgcg ttcggcacgg
     3721 tgtataaggg actctggatc ccagaaggtg agaaagttaa aattcccgtc gctatcaagg
     3781 aattaagaga agcaacatct ccgaaagcca acaaggaaat cctcgatgaa gcctacgtga
     3841 tggccagcgt ggacaacccc cacgtgtgcc gcctgctggg catctgcctc acctccaccg
     3901 tgcagctcat cacgcagctc atgcccttcg gctgcctcct ggactatgtc cgggaacaca
     3961 aagacaatat tggctcccag tacctgctca actggtgtgt gcagatcgca aagggcatga
     4021 actacttgga ggaccgtcgc ttggtgcacc gcgacctggc agccaggaac gtactggtga
     4081 aaacaccgca gcatgtcaag atcacagatt ttgggctggc caaactgctg ggtgcggaag
     4141 agaaagaata ccatgcagaa ggaggcaaag tgcctatcaa gtggatggca ttggaatcaa
     4201 ttttacacag aatctatacc caccagagtg atgtctggag ctacggggtg accgtttggg
     4261 agttgatgac ctttggatcc aagccatatg acggaatccc tgccagcgag atctcctcca
     4321 tcctggagaa aggagaacgc ctccctcagc cacccatatg taccatcgat gtctacatga
     4381 tcatggtcaa gtgctggatg atagacgcag atagtcgccc aaagttccgt gagttgatca
     4441 tcgaattctc caaaatggcc cgagaccccc agcgctacct tgtcattcag ggggatgaaa
     4501 gaatgcattt gccaagtcct acagactcca acttctaccg tgccctgatg gatgaagaag
     4561 acatggacga cgtggtggat gccgacgagt acctcatccc acagcagggc ttcttcagca
     4621 gcccctccac gtcacggact cccctcctga gctctctgag tgcaaccagc aacaattcca
     4681 ccgtggcttg cattgataga aatgggctgc aaagctgtcc catcaaggaa gacagcttct
     4741 tgcagcgata cagctcagac cccacaggcg ccttgactga ggacagcata gacgacacct
     4801 tcctcccagt gcctgaatac ataaaccagt ccgttcccaa aaggcccgct ggctctgtgc
     4861 agaatcctgt ctatcacaat cagcctctga accccgcgcc cagcagagac ccacactacc
     4921 aggaccccca cagcactgca gtgggcaacc ccgagtatct caacactgtc cagcccacct
     4981 gtgtcaacag cacattcgac agccctgccc actgggccca gaaaggcagc caccaaatta
     5041 gcctggacaa ccctgactac cagcaggact tctttcccaa ggaagccaag ccaaatggca
     5101 tctttaaggg ctccacagct gaaaatgcag aatacctaag ggtcgcgcca caaagcagtg
     5161 aatttattgg agcatagaac ccagctttct tgtacaaagt ggtgatatcc aattaaccct
     5221 cactaaagga atattgaaga tgtagtgagt tatccctata gtgagtcgta ttaccaccca
     5281 acttttctat acaaagtggt tgatatccag cacagtggcg gccgctcgag tctagagggc
     5341 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     5401 ggttagtaat gagatccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     5461 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5521 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5581 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5641 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5701 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5761 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5821 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5881 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     5941 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca cacatatgac
     6001 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     6061 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     6121 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     6181 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6241 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6301 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6361 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6421 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6481 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6541 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctaaatcga
     6601 tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6661 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6721 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6781 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6841 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6901 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6961 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7021 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7081 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7141 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7201 agatccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7261 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     7321 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7381 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7441 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7501 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7561 atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa
     7621 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     7681 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     7741 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     7801 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     7861 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     7921 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     7981 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8041 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8101 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8161 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8221 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8281 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8341 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8401 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8461 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     8521 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     8581 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     8641 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     8701 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     8761 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     8821 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     8881 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     8941 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9001 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9061 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9121 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9181 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9241 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9301 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9361 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     9421 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9481 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     9541 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     9601 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     9661 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9721 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9781 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     9841 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     9901 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     9961 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10021 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
    10081 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
    10141 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
    10201 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
    10261 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
    10321 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
    10381 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
    10441 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
    10501 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
    10561 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
    10621 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
    10681 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
    10741 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
    10801 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
    10861 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
    10921 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
    10981 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
    11041 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
    11101 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
    11161 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
    11221 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
    11281 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
    11341 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
    11401 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
    11461 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
    11521 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
    11581 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
    11641 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
    11701 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
    11761 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
    11821 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
    11881 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
    11941 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
    12001 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
    12061 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
    12121 ta
//
