LOCUS       sequence-250696-       12107 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR E746_T751delinsA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12107)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-E746_T751delinsA
FEATURES             Location/Qualifiers
     source          1..12107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    166..283
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        331..1509
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          562..1500
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1457..1477
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1529..1553
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1556..1627,1628..3490,3491..3559,3560..5173)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKASPKANKE
                     ILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNW
                     CVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKV
                     PIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQ
                     PPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTD
                     SNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRN
                     GLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHN
                     QPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPD
                     YQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5175..5199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5207..5225
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5250..5269)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5251..5269)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5275..5295)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5349..5390
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5411..5978
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5428..5980
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5572..5589)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5799..5818
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5992..6591
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5992..6011)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6488..6508
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6605..7193
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6658..6678)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7076..7087)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7268..7501
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7620..7638)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7706..7810
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7811..7879,7880..8671)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8842..9430
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9331..9350
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9584..9601
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9841..9862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9877..9882,9883..9900,9901..9907)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9915..9931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9920..9942
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9939..9955
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9939..9955
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10005..10024
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10216..10849
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10896..11021
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11518..11751
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11936..11980
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
       61 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      121 ctcgacggta tcgccgaatt cacaaatggc agtattcatc cacaatttta aaagaaaagg
      181 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
      241 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
      301 cagcagagat ccagtttgga ctagtcgtga ggctccggtg cccgtcagtg ggcagagcgc
      361 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      421 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      481 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      541 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
      601 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
      661 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
      721 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
      781 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
      841 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
      901 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
      961 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     1021 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     1081 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     1141 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     1201 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     1261 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     1321 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     1381 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     1441 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     1501 gtgtcgtgaa gcggccctgc agatatcaac aagtttgtac aaaaaagcag gcaccatgcg
     1561 accctccggg acggccgggg cagcgctcct ggcgctgctg gctgcgctct gcccggcgag
     1621 tcgggctctg gaggaaaaga aagtttgcca aggcacgagt aacaagctca cgcagttggg
     1681 cacttttgaa gatcattttc tcagcctcca gaggatgttc aataactgtg aggtggtcct
     1741 tgggaatttg gaaattacct atgtgcagag gaattatgat ctttccttct taaagaccat
     1801 ccaggaggtg gctggttatg tcctcattgc cctcaacaca gtggagcgaa ttcctttgga
     1861 aaacctgcag atcatcagag gaaatatgta ctacgaaaat tcctatgcct tagcagtctt
     1921 atctaactat gatgcaaata aaaccggact gaaggagctg cccatgagaa atttacagga
     1981 aatcctgcat ggcgccgtgc ggttcagcaa caaccctgcc ctgtgcaacg tggagagcat
     2041 ccagtggcgg gacatagtca gcagtgactt tctcagcaac atgtcgatgg acttccagaa
     2101 ccacctgggc agctgccaaa agtgtgatcc aagctgtccc aatgggagct gctggggtgc
     2161 aggagaggag aactgccaga aactgaccaa aatcatctgt gcccagcagt gctccgggcg
     2221 ctgccgtggc aagtccccca gtgactgctg ccacaaccag tgtgctgcag gctgcacagg
     2281 cccccgggag agcgactgcc tggtctgccg caaattccga gacgaagcca cgtgcaagga
     2341 cacctgcccc ccactcatgc tctacaaccc caccacgtac cagatggatg tgaaccccga
     2401 gggcaaatac agctttggtg ccacctgcgt gaagaagtgt ccccgtaatt atgtggtgac
     2461 agatcacggc tcgtgcgtcc gagcctgtgg ggccgacagc tatgagatgg aggaagacgg
     2521 cgtccgcaag tgtaagaagt gcgaagggcc ttgccgcaaa gtgtgtaacg gaataggtat
     2581 tggtgaattt aaagactcac tctccataaa tgctacgaat attaaacact tcaaaaactg
     2641 cacctccatc agtggcgatc tccacatcct gccggtggca tttaggggtg actccttcac
     2701 acatactcct cctctggatc cacaggaact ggatattctg aaaaccgtaa aggaaatcac
     2761 agggtttttg ctgattcagg cttggcctga aaacaggacg gacctccatg cctttgagaa
     2821 cctagaaatc atacgcggca ggaccaagca acatggtcag ttttctcttg cagtcgtcag
     2881 cctgaacata acatccttgg gattacgctc cctcaaggag ataagtgatg gagatgtgat
     2941 aatttcagga aacaaaaatt tgtgctatgc aaatacaata aactggaaaa aactgtttgg
     3001 gacctccggt cagaaaacca aaattataag caacagaggt gaaaacagct gcaaggccac
     3061 aggccaggtc tgccatgcct tgtgctcccc cgagggctgc tggggcccgg agcccaggga
     3121 ctgcgtctct tgccggaatg tcagccgagg cagggaatgc gtggacaagt gcaaccttct
     3181 ggagggtgag ccaagggagt ttgtggagaa ctctgagtgc atacagtgcc acccagagtg
     3241 cctgcctcag gccatgaaca tcacctgcac aggacgggga ccagacaact gtatccagtg
     3301 tgcccactac attgacggcc cccactgcgt caagacctgc ccggcaggag tcatgggaga
     3361 aaacaacacc ctggtctgga agtacgcaga cgccggccat gtgtgccacc tgtgccatcc
     3421 aaactgcacc tacggatgca ctgggccagg tcttgaaggc tgtccaacga atgggcctaa
     3481 gatcccgtcc atcgccactg ggatggtggg ggccctcctc ttgctgctgg tggtggccct
     3541 ggggatcggc ctcttcatgc gaaggcgcca catcgttcgg aagcgcacgc tgcggaggct
     3601 gctgcaggag agggagcttg tggagcctct tacacccagt ggagaagctc ccaaccaagc
     3661 tctcttgagg atcttgaagg aaactgaatt caaaaagatc aaagtgctgg gctccggtgc
     3721 gttcggcacg gtgtataagg gactctggat cccagaaggt gagaaagtta aaattcccgt
     3781 cgctatcaag gcatctccga aagccaacaa ggaaatcctc gatgaagcct acgtgatggc
     3841 cagcgtggac aacccccacg tgtgccgcct gctgggcatc tgcctcacct ccaccgtgca
     3901 gctcatcacg cagctcatgc ccttcggctg cctcctggac tatgtccggg aacacaaaga
     3961 caatattggc tcccagtacc tgctcaactg gtgtgtgcag atcgcaaagg gcatgaacta
     4021 cttggaggac cgtcgcttgg tgcaccgcga cctggcagcc aggaacgtac tggtgaaaac
     4081 accgcagcat gtcaagatca cagattttgg gctggccaaa ctgctgggtg cggaagagaa
     4141 agaataccat gcagaaggag gcaaagtgcc tatcaagtgg atggcattgg aatcaatttt
     4201 acacagaatc tatacccacc agagtgatgt ctggagctac ggggtgaccg tttgggagtt
     4261 gatgaccttt ggatccaagc catatgacgg aatccctgcc agcgagatct cctccatcct
     4321 ggagaaagga gaacgcctcc ctcagccacc catatgtacc atcgatgtct acatgatcat
     4381 ggtcaagtgc tggatgatag acgcagatag tcgcccaaag ttccgtgagt tgatcatcga
     4441 attctccaaa atggcccgag acccccagcg ctaccttgtc attcaggggg atgaaagaat
     4501 gcatttgcca agtcctacag actccaactt ctaccgtgcc ctgatggatg aagaagacat
     4561 ggacgacgtg gtggatgccg acgagtacct catcccacag cagggcttct tcagcagccc
     4621 ctccacgtca cggactcccc tcctgagctc tctgagtgca accagcaaca attccaccgt
     4681 ggcttgcatt gatagaaatg ggctgcaaag ctgtcccatc aaggaagaca gcttcttgca
     4741 gcgatacagc tcagacccca caggcgcctt gactgaggac agcatagacg acaccttcct
     4801 cccagtgcct gaatacataa accagtccgt tcccaaaagg cccgctggct ctgtgcagaa
     4861 tcctgtctat cacaatcagc ctctgaaccc cgcgcccagc agagacccac actaccagga
     4921 cccccacagc actgcagtgg gcaaccccga gtatctcaac actgtccagc ccacctgtgt
     4981 caacagcaca ttcgacagcc ctgcccactg ggcccagaaa ggcagccacc aaattagcct
     5041 ggacaaccct gactaccagc aggacttctt tcccaaggaa gccaagccaa atggcatctt
     5101 taagggctcc acagctgaaa atgcagaata cctaagggtc gcgccacaaa gcagtgaatt
     5161 tattggagca tagaacccag ctttcttgta caaagtggtg atatccaatt aaccctcact
     5221 aaaggtgagt aagtttgtat gtttaagtac cctatagtga gtcgtattac cacccaactt
     5281 ttctatacaa agtggttgat atccagcaca gtggcggccg ctcgagtcta gagggcccgc
     5341 ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtt
     5401 agtaatgaga tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     5461 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     5521 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     5581 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     5641 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     5701 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     5761 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     5821 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     5881 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     5941 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaca tatgaccgag
     6001 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     6061 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     6121 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     6181 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     6241 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     6301 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6361 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6421 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6481 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6541 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccta aatcgataga
     6601 tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6661 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6721 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6781 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     6841 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     6901 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     6961 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7021 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7081 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7141 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat
     7201 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     7261 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     7321 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7381 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7441 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7501 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     7561 gagagtgaga ggcccgggtt aattaaggaa agggctagat cattcttgaa gacgaaaggg
     7621 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     7681 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7741 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7801 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7861 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7921 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7981 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8041 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8101 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8161 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8221 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8281 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8341 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8401 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8461 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8521 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8581 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8641 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8701 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8761 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8821 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8881 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8941 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9001 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9061 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     9121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     9181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9481 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9541 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9601 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9661 atgcagcaag ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     9721 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     9781 aagctccccg tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     9841 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     9901 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat
     9961 tacgaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    10021 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
    10081 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
    10141 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
    10201 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
    10261 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
    10321 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
    10381 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
    10441 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
    10501 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
    10561 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
    10621 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
    10681 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10741 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10801 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
    10861 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
    10921 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
    10981 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
    11041 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
    11101 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
    11161 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
    11221 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
    11281 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
    11341 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
    11401 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
    11461 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
    11521 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
    11581 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
    11641 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
    11701 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
    11761 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
    11821 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
    11881 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
    11941 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
    12001 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
    12061 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggat
//
