LOCUS       sequence-250697-       10790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF M517I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10790)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-M517I
FEATURES             Location/Qualifiers
     source          1..10790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..1226
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          279..1217
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1174..1194
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1246..1270
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1271..1280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3575..3599)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3607..3625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3650..3669)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3651..3669)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3675..3695)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3749..3790
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3811..4378
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3828..4380
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3972..3989)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4199..4218
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4392..4991
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4392..4411)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4888..4908
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5005..5593
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5058..5078)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5476..5487)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5668..5901
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6020..6038)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6106..6210
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6211..6279,6280..7071)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6429..6448)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7242..7830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7731..7750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7984..8001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8241..8262
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8277..8282,8283..8300,8301..8307)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8315..8331
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8320..8342
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8339..8355
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8339..8355
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8405..8424
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8616..9249
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9296..9421
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9918..10151
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10336..10380
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10673..10790
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgggtttatt acagggacag cagagatcca gtttggacta gtcgtgaggc tccggtgccc
       61 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      121 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      181 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      241 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      301 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      361 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      421 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      481 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      541 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      601 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      661 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      721 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      781 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      841 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
      901 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
      961 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1021 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1081 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1141 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1201 tttttcttcc atttcaggtg tcgtgaagcg gccctgcaga tatcaacaag tttgtacaaa
     1261 aaagcaggca ccatggcggc gctgagcggt ggcggtggtg gcggcgcgga gccgggccag
     1321 gctctgttca acggggacat ggagcccgag gccggcgccg gcgccggcgc cgcggcctct
     1381 tcggctgcgg accctgccat tccggaggag gtgtggaata tcaaacaaat gattaagttg
     1441 acacaggaac atatagaggc cctattggac aaatttggtg gggagcataa tccaccatca
     1501 atatatctgg aggcctatga agaatacacc agcaagctag atgcactcca acaaagagaa
     1561 caacagttat tggaatctct ggggaacgga actgattttt ctgtttctag ctctgcatca
     1621 atggataccg ttacatcttc ttcctcttct agcctttcag tgctaccttc atctctttca
     1681 gtttttcaaa atcccacaga tgtggcacgg agcaacccca agtcaccaca aaaacctatc
     1741 gttagagtct tcctgcccaa caaacagagg acagtggtac ctgcaaggtg tggagttaca
     1801 gtccgagaca gtctaaagaa agcactgatg atgagaggtc taatcccaga gtgctgtgct
     1861 gtttacagaa ttcaggatgg agagaagaaa ccaattggtt gggacactga tatttcctgg
     1921 cttactggag aagaattgca tgtggaagtg ttggagaatg ttccacttac aacacacaac
     1981 tttgtacgaa aaacgttttt caccttagca ttttgtgact tttgtcgaaa gctgcttttc
     2041 cagggtttcc gctgtcaaac atgtggttat aaatttcacc agcgttgtag tacagaagtt
     2101 ccactgatgt gtgttaatta tgaccaactt gatttgctgt ttgtctccaa gttctttgaa
     2161 caccacccaa taccacagga agaggcgtcc ttagcagaga ctgccctaac atctggatca
     2221 tccccttccg cacccgcctc ggactctatt gggccccaaa ttctcaccag tccgtctcct
     2281 tcaaaatcca ttccaattcc acagcccttc cgaccagcag atgaagatca tcgaaatcaa
     2341 tttgggcaac gagaccgatc ctcatcagct cccaatgtgc atataaacac aatagaacct
     2401 gtcaatattg atgacttgat tagagaccaa ggatttcgtg gtgatggagg atcaaccaca
     2461 ggtttgtctg ctaccccccc tgcctcatta cctggctcac taactaacgt gaaagcctta
     2521 cagaaatctc caggacctca gcgagaaagg aagtcatctt catcctcaga agacaggaat
     2581 cgaatgaaaa cacttggtag acgggactcg agtgatgatt gggagattcc tgatgggcag
     2641 attacagtgg gacaaagaat tggatctgga tcatttggaa cagtctacaa gggaaagtgg
     2701 catggtgatg tggcagtgaa aatgttgaat gtgacagcac ctacacctca gcagttacaa
     2761 gccttcaaaa atgaagtagg agtactcagg aaaacacgac atgtgaatat cctactcttc
     2821 attggctatt ccacaaagcc acaactggct attgttaccc agtggtgtga gggctccagc
     2881 ttgtatcacc atctccatat cattgagacc aaatttgaga tgatcaaact tatagatatt
     2941 gcacgacaga ctgcacaggg catggattac ttacacgcca agtcaatcat ccacagagac
     3001 ctcaagagta ataatatatt tcttcatgaa gacctcacag taaaaatagg tgattttggt
     3061 ctagctacag tgaaatctcg atggagtggg tcccatcagt ttgaacagtt gtctggatcc
     3121 attttgtgga tggcaccaga agtcatcaga atgcaagata aaaatccata cagctttcag
     3181 tcagatgtat atgcatttgg aattgttctg tatgaattga tgactggaca gttaccttat
     3241 tcaaacatca acaacaggga ccagataatt tttatggtgg gacgaggata cctgtctcca
     3301 gatctcagta aggtacggag taactgtcca aaagccatga agagattaat ggcagagtgc
     3361 ctcaaaaaga aaagagatga gagaccactc tttccccaaa ttctcgcctc tattgagctg
     3421 ctggcccgct cattgccaaa aattcaccgc agtgcatcag aaccctcctt gaatcgggct
     3481 ggtttccaaa cagaggattt tagtctatat gcttgtgctt ctccaaaaac acccatccag
     3541 gcagggggat atggtgcgtt tcctgtccac tagaacccag ctttcttgta caaagtggtg
     3601 atatccaatt aaccctcact aaagggttga gaattagaat ttgataaagc cctatagtga
     3661 gtcgtattac cacccaactt ttctatacaa agtggttgat atccagcaca gtggcggccg
     3721 ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct
     3781 cgattctacg cgtaccggtt agtaatgaga tccctccccc ccccctaacg ttactggccg
     3841 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3901 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3961 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     4021 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     4081 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     4141 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     4201 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     4261 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     4321 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4381 tggccacaca tatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     4441 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     4501 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     4561 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     4621 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     4681 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     4741 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     4801 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     4861 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     4921 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     4981 ccggtgccta aatcgataga tcctaatcaa cctctggatt acaaaatttg tgaaagattg
     5041 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5101 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5161 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5221 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5281 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5341 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     5401 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5461 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5521 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     5581 gccgcctccc cgcctgagat cctttaagac caatgactta caaggcagct gtagatctta
     5641 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     5701 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     5761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     5821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     5881 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     5941 ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt aattaaggaa agggctagat
     6001 cattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6061 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6121 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6181 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6241 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6301 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6361 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6421 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
     6481 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6541 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6601 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6661 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6721 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6781 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6841 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6901 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6961 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7021 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7081 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7141 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8041 gcgcgttggc cgattcatta atgcagcaag ctcatggctg actaattttt tttatttatg
     8101 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     8161 gaggcctagg cttttgcaaa aagctccccg tggcacgaca ggtttcccga ctggaaagcg
     8221 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     8281 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     8341 ggaaacagct atgacatgat tacgaatttc acaaataaag catttttttc actgcattct
     8401 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     8461 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     8521 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     8581 tgtatttgtt aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag
     8641 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca
     8701 gaactacaca ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct
     8761 agtaccagtt gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt
     8821 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt
     8881 tgacagccgc ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa
     8941 ctgctgatat cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg
     9001 cctgggcggg actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg
     9061 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9121 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9181 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9241 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     9301 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     9361 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     9421 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     9481 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     9541 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     9601 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     9661 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     9721 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga
     9781 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     9841 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     9901 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     9961 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    10021 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    10081 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    10141 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    10201 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    10261 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    10321 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    10381 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    10441 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    10501 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    10561 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    10621 aacggatctc gacggtatcg ccgaattcac aaatggcagt attcatccac aattttaaaa
    10681 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    10741 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
//
