LOCUS       sequence-250700-       12518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of ERBB3 G40S.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12518)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-ERBB3-G40S
FEATURES             Location/Qualifiers
     source          1..12518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7478..7502)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7510..7528
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7553..7572)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7554..7572)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(7578..7598)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             7652..7693
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    7714..8281
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7731..8283
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7875..7892)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8102..8121
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8295..8894
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8295..8314)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8791..8811
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8908..9496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8961..8981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9379..9390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9571..9804
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9923..9941)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10009..10113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10114..10182,10183..10974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10332..10351)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11145..11733
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11634..11653
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11887..11904
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12144..12165
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12180..12185,12186..12203,12204..12210)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12218..12234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12223..12245
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12242..12258
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12242..12258
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12308..12327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg agggcgaacg acgctctgca ggtgctgggc
     3481 ttgcttttca gcctggcccg gggctccgag gtgggcaact ctcaggcagt gtgtcctggg
     3541 actctgaatg gcctgagtgt gaccagcgat gctgagaacc aataccagac actgtacaag
     3601 ctctacgaga ggtgtgaggt ggtgatgggg aaccttgaga ttgtgctcac gggacacaat
     3661 gccgacctct ccttcctgca gtggattcga gaagtgacag gctatgtcct cgtggccatg
     3721 aatgaattct ctactctacc attgcccaac ctccgcgtgg tgcgagggac ccaggtctac
     3781 gatgggaagt ttgccatctt cgtcatgttg aactataaca ccaactccag ccacgctctg
     3841 cgccagctcc gcttgactca gctcaccgag attctgtcag ggggtgttta tattgagaag
     3901 aacgataagc tttgtcacat ggacacaatt gactggaggg acatcgtgag ggaccgagat
     3961 gctgagatag tggtgaagga caatggcaga agctgtcccc cctgtcatga ggtttgcaag
     4021 gggcgatgct ggggtcctgg atcagaagac tgccagacat tgaccaagac catctgtgct
     4081 cctcagtgta atggtcactg ctttgggccc aaccccaacc agtgctgcca tgatgagtgt
     4141 gccgggggct gctcaggccc tcaggacaca gactgctttg cctgccggca cttcaatgac
     4201 agtggagcct gtgtacctcg ctgtccacag cctcttgtct acaacaagct aactttccag
     4261 ctggaaccca atccccacac caagtatcag tatggaggag tttgtgtagc cagctgtccc
     4321 cataactttg tggtggatca aacatcctgt gtcagggcct gtcctcctga caagatggaa
     4381 gtagataaaa atgggctcaa gatgtgtgag ccttgtgggg gactatgtcc caaagcctgt
     4441 gagggaacag gctctgggag ccgcttccag actgtggact cgagcaacat tgatggattt
     4501 gtgaactgca ccaagatcct gggcaacctg gactttctga tcaccggcct caatggagac
     4561 ccctggcaca agatccctgc cctggaccca gagaagctca atgtcttccg gacagtacgg
     4621 gagatcacag gttacctgaa catccagtcc tggccgcccc acatgcacaa cttcagtgtt
     4681 ttttccaatt tgacaaccat tggaggcaga agcctctaca accggggctt ctcattgttg
     4741 atcatgaaga acttgaatgt cacatctctg ggcttccgat ccctgaagga aattagtgct
     4801 gggcgtatct atataagtgc caataggcag ctctgctacc accactcttt gaactggacc
     4861 aaggtgcttc gggggcctac ggaagagcga ctagacatca agcataatcg gccgcgcaga
     4921 gactgcgtgg cagagggcaa agtgtgtgac ccactgtgct cctctggggg atgctggggc
     4981 ccaggccctg gtcagtgctt gtcctgtcga aattatagcc gaggaggtgt ctgtgtgacc
     5041 cactgcaact ttctgaatgg ggagcctcga gaatttgccc atgaggccga atgcttctcc
     5101 tgccacccgg aatgccaacc catggagggc actgccacat gcaatggctc gggctctgat
     5161 acttgtgctc aatgtgccca ttttcgagat gggccccact gtgtgagcag ctgcccccat
     5221 ggagtcctag gtgccaaggg cccaatctac aagtacccag atgttcagaa tgaatgtcgg
     5281 ccctgccatg agaactgcac ccaggggtgt aaaggaccag agcttcaaga ctgtttagga
     5341 caaacactgg tgctgatcgg caaaacccat ctgacaatgg ctttgacagt gatagcagga
     5401 ttggtagtga ttttcatgat gctgggcggc acttttctct actggcgtgg gcgccggatt
     5461 cagaataaaa gggctatgag gcgatacttg gaacggggtg agagcataga gcctctggac
     5521 cccagtgaga aggctaacaa agtcttggcc agaatcttca aagagacaga gctaaggaag
     5581 cttaaagtgc ttggctcggg tgtctttgga actgtgcaca aaggagtgtg gatccctgag
     5641 ggtgaatcaa tcaagattcc agtctgcatt aaagtcattg aggacaagag tggacggcag
     5701 agttttcaag ctgtgacaga tcatatgctg gccattggca gcctggacca tgcccacatt
     5761 gtaaggctgc tgggactatg cccagggtca tctctgcagc ttgtcactca atatttgcct
     5821 ctgggttctc tgctggatca tgtgagacaa caccgggggg cactggggcc acagctgctg
     5881 ctcaactggg gagtacaaat tgccaaggga atgtactacc ttgaggaaca tggtatggtg
     5941 catagaaacc tggctgcccg aaacgtgcta ctcaagtcac ccagtcaggt tcaggtggca
     6001 gattttggtg tggctgacct gctgcctcct gatgataagc agctgctata cagtgaggcc
     6061 aagactccaa ttaagtggat ggcccttgag agtatccact ttgggaaata cacacaccag
     6121 agtgatgtct ggagctatgg tgtgacagtt tgggagttga tgaccttcgg ggcagagccc
     6181 tatgcagggc tacgattggc tgaagtacca gacctgctag agaaggggga gcggttggca
     6241 cagccccaga tctgcacaat tgatgtctac atggtgatgg tcaagtgttg gatgattgat
     6301 gagaacattc gcccaacctt taaagaacta gccaatgagt tcaccaggat ggcccgagac
     6361 ccaccacggt atctggtcat aaagagagag agtgggcctg gaatagcccc tgggccagag
     6421 ccccatggtc tgacaaacaa gaagctagag gaagtagagc tggagccaga actagaccta
     6481 gacctagact tggaagcaga ggaggacaac ctggcaacca ccacactggg ctccgccctc
     6541 agcctaccag ttggaacact taatcggcca cgtgggagcc agagcctttt aagtccatca
     6601 tctggataca tgcccatgaa ccagggtaat cttggggagt cttgccagga gtctgcagtt
     6661 tctgggagca gtgaacggtg cccccgtcca gtctctctac acccaatgcc acggggatgc
     6721 ctggcatcag agtcatcaga ggggcatgta acaggctctg aggctgagct ccaggagaaa
     6781 gtgtcaatgt gtaggagccg gagcaggagc cggagcccac ggccacgcgg agatagcgcc
     6841 taccattccc agcgccacag tctgctgact cctgttaccc cactctcccc acccgggtta
     6901 gaggaagagg atgtcaacgg ttatgtcatg ccagatacac acctcaaagg tactccctcc
     6961 tcccgggaag gcaccctttc ttcagtgggt ctcagttctg tcctgggtac tgaagaagaa
     7021 gatgaagatg aggagtatga atacatgaac cggaggagaa ggcacagtcc acctcatccc
     7081 cctaggccaa gttcccttga ggagctgggt tatgagtaca tggatgtggg gtcagacctc
     7141 agtgcctctc tgggcagcac acagagttgc ccactccacc ctgtacccat catgcccact
     7201 gcaggcacaa ctccagatga agactatgaa tatatgaatc ggcaacgaga tggaggtggt
     7261 cctgggggtg attatgcagc catgggggcc tgcccagcat ctgagcaagg gtatgaagag
     7321 atgagagctt ttcaggggcc tggacatcag gccccccatg tccattatgc ccgcctaaaa
     7381 actctacgta gcttagaggc tacagactct gcctttgata accctgatta ctggcatagc
     7441 aggcttttcc ccaaggctaa tgcccagaga acgtagaacc cagctttctt gtacaaagtg
     7501 gtgatatcca attaaccctc actaaaggtt gtatgtgagt ttagattaat gaccctatag
     7561 tgagtcgtat taccacccaa cttttctata caaagtggtt gatatccagc acagtggcgg
     7621 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     7681 tctcgattct acgcgtaccg gttagtaatg agatccctcc cccccccnta acgttactgg
     7741 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     7801 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     7861 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     7921 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     7981 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     8041 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     8101 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     8161 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     8221 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     8281 atatggccac acatatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     8341 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     8401 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     8461 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     8521 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     8581 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     8641 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     8701 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     8761 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     8821 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     8881 agcccggtgc ctaaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     8941 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     9001 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     9061 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     9121 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     9181 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     9241 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     9301 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     9361 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     9421 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     9481 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     9541 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     9601 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     9661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     9721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9781 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     9841 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     9901 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     9961 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
    10021 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
    10081 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
    10141 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
    10201 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
    10261 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
    10321 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
    10381 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
    10441 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
    10501 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
    10561 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
    10621 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
    10681 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
    10741 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
    10801 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
    10861 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
    10921 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
    10981 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
    11041 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    11101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    11161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    11221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    11281 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    11341 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    11401 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    11461 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    11521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    11581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    11641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    11701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    11761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    11821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    11881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    11941 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
    12001 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
    12061 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
    12121 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    12181 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    12241 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
    12301 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
    12361 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
    12421 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    12481 aattgtattt gttaaatatg tactacaaac ttagtagt
//
