LOCUS       sequence-250701-       10789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF P403Lfs*8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10789)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-P403Lfs*8
FEATURES             Location/Qualifiers
     source          1..10789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1389..1398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3692..3716)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3724..3742
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3767..3786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3768..3786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3792..3812)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             3866..3907
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3928..4495
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3945..4497
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4089..4106)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4316..4335
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4509..5108
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4509..4528)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5005..5025
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5122..5710
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5175..5195)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5593..5604)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5785..6018
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6137..6155)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6223..6327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6328..6396,6397..7188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6546..6565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7359..7947
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7848..7867
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8101..8118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8358..8379
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8394..8399,8400..8417,8418..8424)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8432..8448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8437..8459
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8456..8472
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8456..8472
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8522..8541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8733..9366
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9413..9538
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10035..10268
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10453..10497
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc
     1441 tctgttcaac ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc
     1501 ggctgcggac cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac
     1561 acaggaacat atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat
     1621 atatctggag gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca
     1681 acagttattg gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat
     1741 ggataccgtt acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt
     1801 ttttcaaaat cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt
     1861 tagagtcttc ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt
     1921 ccgagacagt ctaaagaaag cactgatgat gagaggtcta atcccagagt gctgtgctgt
     1981 ttacagaatt caggatggag agaagaaacc aattggttgg gacactgata tttcctggct
     2041 tactggagaa gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt
     2101 tgtacgaaaa acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca
     2161 gggtttccgc tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc
     2221 actgatgtgt gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca
     2281 ccacccaata ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc
     2341 cccttccgca cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc
     2401 aaaatccatt ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt
     2461 tgggcaacga gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt
     2521 caatattgat gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg
     2581 tttgtctgct acccccctgc ctcattacct ggctcactaa ctaacgtgaa agccttacag
     2641 aaatctccag gacctcagcg agaaaggaag tcatcttcat cctcagaaga caggaatcga
     2701 atgaaaacac ttggtagacg ggactcgagt gatgattggg agattcctga tgggcagatt
     2761 acagtgggac aaagaattgg atctggatca tttggaacag tctacaaggg aaagtggcat
     2821 ggtgatgtgg cagtgaaaat gttgaatgtg acagcaccta cacctcagca gttacaagcc
     2881 ttcaaaaatg aagtaggagt actcaggaaa acacgacatg tgaatatcct actcttcatg
     2941 ggctattcca caaagccaca actggctatt gttacccagt ggtgtgaggg ctccagcttg
     3001 tatcaccatc tccatatcat tgagaccaaa tttgagatga tcaaacttat agatattgca
     3061 cgacagactg cacagggcat ggattactta cacgccaagt caatcatcca cagagacctc
     3121 aagagtaata atatatttct tcatgaagac ctcacagtaa aaataggtga ttttggtcta
     3181 gctacagtga aatctcgatg gagtgggtcc catcagtttg aacagttgtc tggatccatt
     3241 ttgtggatgg caccagaagt catcagaatg caagataaaa atccatacag ctttcagtca
     3301 gatgtatatg catttggaat tgttctgtat gaattgatga ctggacagtt accttattca
     3361 aacatcaaca acagggacca gataattttt atggtgggac gaggatacct gtctccagat
     3421 ctcagtaagg tacggagtaa ctgtccaaaa gccatgaaga gattaatggc agagtgcctc
     3481 aaaaagaaaa gagatgagag accactcttt ccccaaattc tcgcctctat tgagctgctg
     3541 gcccgctcat tgccaaaaat tcaccgcagt gcatcagaac cctccttgaa tcgggctggt
     3601 ttccaaacag aggattttag tctatatgct tgtgcttctc caaaaacacc catccaggca
     3661 gggggatatg gtgcgtttcc tgtccactag aacccagctt tcttgtacaa agtggtgata
     3721 tccaattaac cctcactaaa gggataagaa agtgaaatgt aaattgccct atagtgagtc
     3781 gtattaccac ccaacttttc tatacaaagt ggttgatatc cagcacagtg gcggccgctc
     3841 gagtctagag ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga
     3901 ttctacgcgt accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag
     3961 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     4021 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4081 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4141 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4201 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4261 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4321 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4381 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4441 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     4501 ccacacatat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     4561 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     4621 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     4681 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     4741 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     4801 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     4861 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     4921 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     4981 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     5041 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     5101 gtgcctaaat cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
     5161 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5221 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5281 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5341 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5401 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     5461 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     5521 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     5581 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     5641 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     5701 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5761 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5821 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5881 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5941 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6001 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6061 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
     6121 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     6181 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6241 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6301 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6361 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6421 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6481 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6541 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     6601 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6661 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6721 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6781 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6841 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6901 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6961 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7021 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7081 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7141 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7201 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7261 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7321 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7381 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7441 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7501 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7561 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7621 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7681 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7741 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7801 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7861 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7921 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7981 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8041 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8101 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8161 cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag
     8221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8281 gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc
     8341 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8401 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8461 aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt
     8521 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     8581 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     8641 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     8701 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     8761 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     8821 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     8881 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     8941 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     9001 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     9061 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     9121 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     9181 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     9241 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     9301 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     9361 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     9421 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     9481 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     9541 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     9601 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     9661 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     9721 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     9781 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     9841 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
     9901 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     9961 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
    10021 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
    10081 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
    10141 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
    10201 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
    10261 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
    10321 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
    10381 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
    10441 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
    10501 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
    10561 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
    10621 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
    10681 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
    10741 ggatctcgac ggtatcgccg aattcacaaa tggcagtatt catccacaa
//
