LOCUS       sequence-250703-       12104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR L747_P753delinsS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-L747_P753delinsS
FEATURES             Location/Qualifiers
     source          1..12104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..45
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    338..455
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        503..1681
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          734..1672
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1629..1649
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1701..1725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1728..1799,1800..3662,3663..3731,3732..5342)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKESKANKEI
                     LDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWC
                     VQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVP
                     IKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQP
                     PICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDS
                     NFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNG
                     LQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQ
                     PLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDY
                     QQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5344..5368)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5376..5394
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5419..5438)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5420..5438)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5444..5464)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5518..5559
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5580..6147
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5597..6149
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5741..5758)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5968..5987
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6161..6760
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6161..6180)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6657..6677
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6774..7362
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6827..6847)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7245..7256)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7437..7670
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7789..7807)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7875..7979
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7980..8048,8049..8840)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9011..9599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9500..9519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9753..9770
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10010..10031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10046..10051,10052..10069,10070..10076)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10084..10100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10089..10111
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10108..10124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10108..10124
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10174..10193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10385..11018
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11065..11190
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11687..11920
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
       61 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
      121 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
      181 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
      241 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
      301 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      361 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      421 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      481 atccagtttg gactagtcgt gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      541 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
      601 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
      661 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
      721 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
      781 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
      841 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
      901 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
      961 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     1021 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     1081 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     1141 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     1201 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     1261 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     1321 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1381 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1441 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1501 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1561 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1621 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     1681 aagcggccct gcagatatca acaagtttgt acaaaaaagc aggcaccatg cgaccctccg
     1741 ggacggccgg ggcagcgctc ctggcgctgc tggctgcgct ctgcccggcg agtcgggctc
     1801 tggaggaaaa gaaagtttgc caaggcacga gtaacaagct cacgcagttg ggcacttttg
     1861 aagatcattt tctcagcctc cagaggatgt tcaataactg tgaggtggtc cttgggaatt
     1921 tggaaattac ctatgtgcag aggaattatg atctttcctt cttaaagacc atccaggagg
     1981 tggctggtta tgtcctcatt gccctcaaca cagtggagcg aattcctttg gaaaacctgc
     2041 agatcatcag aggaaatatg tactacgaaa attcctatgc cttagcagtc ttatctaact
     2101 atgatgcaaa taaaaccgga ctgaaggagc tgcccatgag aaatttacag gaaatcctgc
     2161 atggcgccgt gcggttcagc aacaaccctg ccctgtgcaa cgtggagagc atccagtggc
     2221 gggacatagt cagcagtgac tttctcagca acatgtcgat ggacttccag aaccacctgg
     2281 gcagctgcca aaagtgtgat ccaagctgtc ccaatgggag ctgctggggt gcaggagagg
     2341 agaactgcca gaaactgacc aaaatcatct gtgcccagca gtgctccggg cgctgccgtg
     2401 gcaagtcccc cagtgactgc tgccacaacc agtgtgctgc aggctgcaca ggcccccggg
     2461 agagcgactg cctggtctgc cgcaaattcc gagacgaagc cacgtgcaag gacacctgcc
     2521 ccccactcat gctctacaac cccaccacgt accagatgga tgtgaacccc gagggcaaat
     2581 acagctttgg tgccacctgc gtgaagaagt gtccccgtaa ttatgtggtg acagatcacg
     2641 gctcgtgcgt ccgagcctgt ggggccgaca gctatgagat ggaggaagac ggcgtccgca
     2701 agtgtaagaa gtgcgaaggg ccttgccgca aagtgtgtaa cggaataggt attggtgaat
     2761 ttaaagactc actctccata aatgctacga atattaaaca cttcaaaaac tgcacctcca
     2821 tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc acacatactc
     2881 ctcctctgga tccacaggaa ctggatattc tgaaaaccgt aaaggaaatc acagggtttt
     2941 tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag aacctagaaa
     3001 tcatacgcgg caggaccaag caacatggtc agttttctct tgcagtcgtc agcctgaaca
     3061 taacatcctt gggattacgc tccctcaagg agataagtga tggagatgtg ataatttcag
     3121 gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt gggacctccg
     3181 gtcagaaaac caaaattata agcaacagag gtgaaaacag ctgcaaggcc acaggccagg
     3241 tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg gactgcgtct
     3301 cttgccggaa tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg
     3361 agccaaggga gtttgtggag aactctgagt gcatacagtg ccacccagag tgcctgcctc
     3421 aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag tgtgcccact
     3481 acattgacgg cccccactgc gtcaagacct gcccggcagg agtcatggga gaaaacaaca
     3541 ccctggtctg gaagtatgca gacgccggcc atgtgtgcca cctgtgccat ccaaactgca
     3601 cctacggatg cactgggcca ggtcttgaag gctgtccaac gaatgggcct aagatcccgt
     3661 ccatcgccac tgggatggtg ggggccctcc tcttgctgct ggtggtggcc ctggggatcg
     3721 gcctcttcat gcgaaggcgc cacatcgttc ggaagcgcac gctgcggagg ctgctgcagg
     3781 agagggagct tgtggagcct cttacaccca gtggagaagc tcccaaccaa gctctcttga
     3841 ggatcttgaa ggaaactgaa ttcaaaaaga tcaaagtgct gggctccggt gcgttcggca
     3901 cggtgtataa gggactctgg atcccagaag gtgagaaagt taaaattccc gtcgctatca
     3961 aggaatcgaa agccaacaag gaaatcctcg atgaagccta cgtgatggcc agcgtggaca
     4021 acccccacgt gtgccgcctg ctgggcatct gcctcacctc caccgtgcag ctcatcacgc
     4081 agctcatgcc cttcggctgc ctcctggact atgtccggga acacaaagac aatattggct
     4141 cccagtacct gctcaactgg tgtgtgcaga tcgcaaaggg catgaactac ttggaggacc
     4201 gtcgcttggt gcaccgcgac ctggcagcca ggaacgtact ggtgaaaaca ccgcagcatg
     4261 tcaagatcac agattttggg ctggccaaac tgctgggtgc ggaagagaaa gaataccatg
     4321 cagaaggagg caaagtgcct atcaagtgga tggcattgga atcaatttta cacagaatct
     4381 atacccacca gagtgatgtc tggagctacg gggtgaccgt ttgggagttg atgacctttg
     4441 gatccaagcc atatgacgga atccctgcca gcgagatctc ctccatcctg gagaaaggag
     4501 aacgcctccc tcagccaccc atatgtacca tcgatgtcta catgatcatg gtcaagtgct
     4561 ggatgataga cgcagatagt cgcccaaagt tccgtgagtt gatcatcgaa ttctccaaaa
     4621 tggcccgaga cccccagcgc taccttgtca ttcaggggga tgaaagaatg catttgccaa
     4681 gtcctacaga ctccaacttc taccgtgccc tgatggatga agaagacatg gacgacgtgg
     4741 tggatgccga cgagtacctc atcccacagc agggcttctt cagcagcccc tccacgtcac
     4801 ggactcccct cctgagctct ctgagtgcaa ccagcaacaa ttccaccgtg gcttgcattg
     4861 atagaaatgg gctgcaaagc tgtcccatca aggaagacag cttcttgcag cgatacagct
     4921 cagaccccac aggcgccttg actgaggaca gcatagacga caccttcctc ccagtgcctg
     4981 aatacataaa ccagtccgtt cccaaaaggc ccgctggctc tgtgcagaat cctgtctatc
     5041 acaatcagcc tctgaacccc gcgcccagca gagacccaca ctaccaggac ccccacagca
     5101 ctgcagtggg caaccccgag tatctcaaca ctgtccagcc cacctgtgtc aacagcacat
     5161 tcgacagccc tgcccactgg gcccagaaag gcagccacca aattagcctg gacaaccctg
     5221 actaccagca ggacttcttt cccaaggaag ccaagccaaa tggcatcttt aagggctcca
     5281 cagctgaaaa tgcagaatac ctaagggtcg cgccacaaag cagtgaattt attggagcat
     5341 agaacccagc tttcttgtac aaagtggtga tatccaatta accctcacta aaggtttgtg
     5401 tgttattgta attgagatcc ctatagtgag tcgtattacc acccaacttt tctatacaaa
     5461 gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt
     5521 aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagat
     5581 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     5641 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     5701 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     5761 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     5821 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     5881 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5941 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     6001 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     6061 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     6121 gttttccttt gaaaaacacg atgataatat ggccacacat atgaccgagt acaagcccac
     6181 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     6241 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     6301 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     6361 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     6421 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     6481 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     6541 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     6601 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     6661 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     6721 accgcgcacc tggtgcatga cccgcaagcc cggtgcctaa atcgatagat cctaatcaac
     6781 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6841 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     6901 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     6961 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7021 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7081 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7141 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     7201 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7261 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     7321 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc
     7381 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7441 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7501 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7561 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7621 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7681 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7741 gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac
     7801 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7861 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7921 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7981 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8041 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8101 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8161 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8221 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8281 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8341 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8401 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8461 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8521 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8581 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8641 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8701 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8761 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8821 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8881 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8941 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9001 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9061 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9121 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9181 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9241 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9301 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9361 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9421 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9481 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9541 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     9601 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     9661 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     9721 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     9781 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc
     9841 tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     9901 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt
     9961 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
    10021 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
    10081 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca
    10141 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10201 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
    10261 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
    10321 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
    10381 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
    10441 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
    10501 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
    10561 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
    10621 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
    10681 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
    10741 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
    10801 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
    10861 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    10921 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    10981 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
    11041 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
    11101 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
    11161 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
    11221 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
    11281 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
    11341 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
    11401 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
    11461 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
    11521 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
    11581 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
    11641 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
    11701 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
    11761 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    11821 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    11881 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    11941 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    12001 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
    12061 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaa
//
