LOCUS       sequence-250705-       10813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of BRAF N486D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10813)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 10813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-BRAF-N486D
FEATURES             Location/Qualifiers
     source          1..10813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..118
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        166..1344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          397..1335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1292..1312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1364..1388
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1389..1398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3693..3717)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3770..3811
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3832..4399
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3849..4401
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3993..4010)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4220..4239
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4413..4415,4416..4418,4419..5132)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4413..4415,4416..4418,4419..5129)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4458..4479)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4719..4738)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5066..5087
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5146..5734
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5199..5219)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5617..5628)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5809..6042
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6161..6179)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6247..6351
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6352..6420,6421..7212)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6570..6589)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7383..7971
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7872..7891
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8125..8142
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8382..8403
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8418..8423,8424..8441,8442..8448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8456..8472
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8461..8483
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8480..8496
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8480..8496
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8546..8565
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8757..9390
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9437..9562
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10059..10292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10477..10521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
       61 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
      121 ggtttattac agggacagca gagatccagt ttggactagt cgtgaggctc cggtgcccgt
      181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     1021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     1081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1321 tttcttccat ttcaggtgtc gtgaagcggc cctgcagata tcaacaagtt tgtacaaaaa
     1381 agcaggcacc atggcggcgc tgagcggtgg cggtggtggc ggcgcggagc cgggccaggc
     1441 tctgttcaac ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc
     1501 ggctgcggac cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac
     1561 acaggaacat atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat
     1621 atatctggag gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca
     1681 acagttattg gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat
     1741 ggataccgtt acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt
     1801 ttttcaaaat cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt
     1861 tagagtcttc ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt
     1921 ccgagacagt ctaaagaaag cactgatgat gagaggtcta atcccagagt gctgtgctgt
     1981 ttacagaatt caggatggag agaagaaacc aattggttgg gacactgata tttcctggct
     2041 tactggagaa gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt
     2101 tgtacgaaaa acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca
     2161 gggtttccgc tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc
     2221 actgatgtgt gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca
     2281 ccacccaata ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc
     2341 cccttccgca cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc
     2401 aaaatccatt ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt
     2461 tgggcaacga gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt
     2521 caatattgat gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg
     2581 tttgtctgct accccccctg cctcattacc tggctcacta actaacgtga aagccttaca
     2641 gaaatctcca ggacctcagc gagaaaggaa gtcatcttca tcctcagaag acaggaatcg
     2701 aatgaaaaca cttggtagac gggactcgag tgatgattgg gagattcctg atgggcagat
     2761 tacagtggga caaagaattg gatctggatc atttggaaca gtctacaagg gaaagtggca
     2821 tggtgatgtg gcagtgaaaa tgttggatgt gacagcacct acacctcagc agttacaagc
     2881 cttcaaaaat gaagtaggag tactcaggaa aacacgacat gtgaatatcc tactcttcat
     2941 gggctattcc acaaagccac aactggctat tgttacccag tggtgtgagg gctccagctt
     3001 gtatcaccat ctccatatca ttgagaccaa atttgagatg atcaaactta tagatattgc
     3061 acgacagact gcacagggca tggattactt acacgccaag tcaatcatcc acagagacct
     3121 caagagtaat aatatatttc ttcatgaaga cctcacagta aaaataggtg attttggtct
     3181 agctacagtg aaatctcgat ggagtgggtc ccatcagttt gaacagttgt ctggatccat
     3241 tttgtggatg gcaccagaag tcatcagaat gcaagataaa aatccataca gctttcagtc
     3301 agatgtatat gcatttggaa ttgttctgta tgaattgatg actggacagt taccttattc
     3361 aaacatcaac aacagggacc agataatttt tatggtggga cgaggatacc tgtctccaga
     3421 tctcagtaag gtacggagta actgtccaaa agccatgaag agattaatgg cagagtgcct
     3481 caaaaagaaa agagatgaga gaccactctt tccccaaatt ctcgcctcta ttgagctgct
     3541 ggcccgctca ttgccaaaaa ttcaccgcag tgcatcagaa ccctccttga atcgggctgg
     3601 tttccaaaca gaggatttta gtctatatgc ttgtgcttct ccaaaaacac ccatccaggc
     3661 agggggatat ggtgcgtttc ctgtccacta gaacccagct ttcttgtaca aagtggttga
     3721 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat
     3781 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt tagtaatgag atccctcccc
     3841 cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat
     3901 gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac ctggccctgt
     3961 cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt
     4021 gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa cgtctgtagc
     4081 gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg gccaaaagcc
     4141 acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg tgagttggat
     4201 agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc tgaaggatgc
     4261 ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat gctttacatg
     4321 tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg tggttttcct
     4381 ttgaaaaaca cgatgataat atggccacac atatggtgag caagggcgag gagctgttca
     4441 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     4501 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     4561 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     4621 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     4681 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     4741 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     4801 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     4861 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     4921 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     4981 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     5041 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5101 tcactctcgg catggacgag ctgtacaagt aaatcgatag atcctaatca acctctggat
     5161 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5221 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5281 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5341 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5401 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5461 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5521 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     5581 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5641 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5701 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
     5761 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     5821 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     5881 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     5941 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6001 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6061 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
     6121 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
     6181 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     6241 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6301 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     6361 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     6421 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     6481 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     6541 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     6601 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     6661 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     6721 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     6781 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6841 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     6901 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6961 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     7021 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     7081 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     7141 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     7201 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     7261 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     7321 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     7381 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     7441 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     7501 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     7561 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     7621 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     7681 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     7741 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     7801 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7861 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7921 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7981 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     8041 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     8101 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     8161 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa gctcatggct
     8221 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8281 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc gtggcacgac
     8341 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8401 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8461 agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt cacaaataaa
     8521 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8581 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
     8641 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
     8701 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     8761 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     8821 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     8881 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     8941 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     9001 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     9061 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     9121 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     9181 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     9241 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9301 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9361 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     9421 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     9481 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     9541 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     9601 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     9661 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     9721 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     9781 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     9841 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     9901 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     9961 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
    10021 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
    10081 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
    10141 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
    10201 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
    10261 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
    10321 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
    10381 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
    10441 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
    10501 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
    10561 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
    10621 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
    10681 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
    10741 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
    10801 tattcatcca caa
//
