LOCUS       sequence-250711-       12128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of EGFR M766_A767insAI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 12128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-EGFR-M766_A767insAI
FEATURES             Location/Qualifiers
     source          1..12128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    274..391
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        439..1617
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          670..1608
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1565..1585
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1637..1661
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1664..1735,1736..3598,3599..3667,3668..5302)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMAIASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIG
                     SQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEY
                     HAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILE
                     KGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERM
                     HLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNST
                     VACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSV
                     QNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQ
                     ISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     protein_bind    complement(5304..5328)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5336..5354
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5379..5398)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5380..5398)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5404..5424)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             5478..5519
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    5540..6107
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5557..6109
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5701..5718)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5928..5947
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6121..6720
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6121..6140)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6617..6637
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6734..7322
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6787..6807)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7205..7216)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7397..7630
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7749..7767)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7835..7939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7940..8008,8009..8800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8971..9559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9460..9479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9713..9730
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9970..9991
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10006..10011,10012..10029,10030..10036)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10044..10060
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10049..10071
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10068..10084
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10068..10084
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10134..10153
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             10345..10978
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    11025..11150
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11647..11880
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12065..12109
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
       61 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
      121 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
      181 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
      241 gccgaattca caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
      301 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
      361 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
      421 agtttggact agtcgtgagg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
      481 gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
      541 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
      601 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
      661 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
      721 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
      781 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
      841 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
      901 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
      961 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     1021 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     1081 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     1141 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     1201 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     1261 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     1321 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     1381 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     1441 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     1501 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     1561 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgaagc
     1621 ggccctgcag atatcaacaa gtttgtacaa aaaagcaggc accatgcgac cctccgggac
     1681 ggccggggca gcgctcctgg cgctgctggc tgcgctctgc ccggcgagtc gggctctgga
     1741 ggaaaagaaa gtttgccaag gcacgagtaa caagctcacg cagttgggca cttttgaaga
     1801 tcattttctc agcctccaga ggatgttcaa taactgtgag gtggtccttg ggaatttgga
     1861 aattacctat gtgcagagga attatgatct ttccttctta aagaccatcc aggaggtggc
     1921 tggttatgtc ctcattgccc tcaacacagt ggagcgaatt cctttggaaa acctgcagat
     1981 catcagagga aatatgtact acgaaaattc ctatgcctta gcagtcttat ctaactatga
     2041 tgcaaataaa accggactga aggagctgcc catgagaaat ttacaggaaa tcctgcatgg
     2101 cgccgtgcgg ttcagcaaca accctgccct gtgcaacgtg gagagcatcc agtggcggga
     2161 catagtcagc agtgactttc tcagcaacat gtcgatggac ttccagaacc acctgggcag
     2221 ctgccaaaag tgtgatccaa gctgtcccaa tgggagctgc tggggtgcag gagaggagaa
     2281 ctgccagaaa ctgaccaaaa tcatctgtgc ccagcagtgc tccgggcgct gccgtggcaa
     2341 gtcccccagt gactgctgcc acaaccagtg tgctgcaggc tgcacaggcc cccgggagag
     2401 cgactgcctg gtctgccgca aattccgaga cgaagccacg tgcaaggaca cctgcccccc
     2461 actcatgctc tacaacccca ccacgtacca gatggatgtg aaccccgagg gcaaatacag
     2521 ctttggtgcc acctgcgtga agaagtgtcc ccgtaattat gtggtgacag atcacggctc
     2581 gtgcgtccga gcctgtgggg ccgacagcta tgagatggag gaagacggcg tccgcaagtg
     2641 taagaagtgc gaagggcctt gccgcaaagt gtgtaacgga ataggtattg gtgaatttaa
     2701 agactcactc tccataaatg ctacgaatat taaacacttc aaaaactgca cctccatcag
     2761 tggcgatctc cacatcctgc cggtggcatt taggggtgac tccttcacac atactcctcc
     2821 tctggatcca caggaactgg atattctgaa aaccgtaaag gaaatcacag ggtttttgct
     2881 gattcaggct tggcctgaaa acaggacgga cctccatgcc tttgagaacc tagaaatcat
     2941 acgcggcagg accaagcaac atggtcagtt ttctcttgca gtcgtcagcc tgaacataac
     3001 atccttggga ttacgctccc tcaaggagat aagtgatgga gatgtgataa tttcaggaaa
     3061 caaaaatttg tgctatgcaa atacaataaa ctggaaaaaa ctgtttggga cctccggtca
     3121 gaaaaccaaa attataagca acagaggtga aaacagctgc aaggccacag gccaggtctg
     3181 ccatgccttg tgctcccccg agggctgctg gggcccggag cccagggact gcgtctcttg
     3241 ccggaatgtc agccgaggca gggaatgcgt ggacaagtgc aaccttctgg agggtgagcc
     3301 aagggagttt gtggagaact ctgagtgcat acagtgccac ccagagtgcc tgcctcaggc
     3361 catgaacatc acctgcacag gacggggacc agacaactgt atccagtgtg cccactacat
     3421 tgacggcccc cactgcgtca agacctgccc ggcaggagtc atgggagaaa acaacaccct
     3481 ggtctggaag tacgcagacg ccggccatgt gtgccacctg tgccatccaa actgcaccta
     3541 cggatgcact gggccaggtc ttgaaggctg tccaacgaat gggcctaaga tcccgtccat
     3601 cgccactggg atggtggggg ccctcctctt gctgctggtg gtggccctgg ggatcggcct
     3661 cttcatgcga aggcgccaca tcgttcggaa gcgcacgctg cggaggctgc tgcaggagag
     3721 ggagcttgtg gagcctctta cacccagtgg agaagctccc aaccaagctc tcttgaggat
     3781 cttgaaggaa actgaattca aaaagatcaa agtgctgggc tccggtgcgt tcggcacggt
     3841 gtataaggga ctctggatcc cagaaggtga gaaagttaaa attcccgtcg ctatcaagga
     3901 attaagagaa gcaacatctc cgaaagccaa caaggaaatc ctcgatgaag cctacgtgat
     3961 ggcaatcgcc agcgtggaca acccccacgt gtgccgcctg ctgggcatct gcctcacctc
     4021 caccgtgcag ctcatcacgc agctcatgcc cttcggctgc ctcctggact atgtccggga
     4081 acacaaagac aatattggct cccagtacct gctcaactgg tgtgtgcaga tcgcaaaggg
     4141 catgaactac ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca ggaacgtact
     4201 ggtgaaaaca ccgcagcatg tcaagatcac agattttggg ctggccaaac tgctgggtgc
     4261 ggaagagaaa gaataccatg cagaaggagg caaagtgcct atcaagtgga tggcattgga
     4321 atcaatttta cacagaatct atacccacca gagtgatgtc tggagctacg gggtgaccgt
     4381 ttgggagttg atgacctttg gatccaagcc atatgacgga atccctgcca gcgagatctc
     4441 ctccatcctg gagaaaggag aacgcctccc tcagccaccc atatgtacca tcgatgtcta
     4501 catgatcatg gtcaagtgct ggatgataga cgcagatagt cgcccaaagt tccgtgagtt
     4561 gatcatcgaa ttctccaaaa tggcccgaga cccccagcgc taccttgtca ttcaggggga
     4621 tgaaagaatg catttgccaa gtcctacaga ctccaacttc taccgtgccc tgatggatga
     4681 agaagacatg gacgacgtgg tggatgccga cgagtacctc atcccacagc agggcttctt
     4741 cagcagcccc tccacgtcac ggactcccct cctgagctct ctgagtgcaa ccagcaacaa
     4801 ttccaccgtg gcttgcattg atagaaatgg gctgcaaagc tgtcccatca aggaagacag
     4861 cttcttgcag cgatacagct cagaccccac aggcgccttg actgaggaca gcatagacga
     4921 caccttcctc ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc ccgctggctc
     4981 tgtgcagaat cctgtctatc acaatcagcc tctgaacccc gcgcccagca gagacccaca
     5041 ctaccaggac ccccacagca ctgcagtggg caaccccgag tatctcaaca ctgtccagcc
     5101 cacctgtgtc aacagcacat tcgacagccc tgcccactgg gcccagaaag gcagccacca
     5161 aattagcctg gacaaccctg actaccagca ggacttcttt cccaaggaag ccaagccaaa
     5221 tggcatcttt aagggctcca cagctgaaaa tgcagaatac ctaagggtcg cgccacaaag
     5281 cagtgaattt attggagcat agaacccagc tttcttgtac aaagtggtga tatccaatta
     5341 accctcacta aagggttatg atatagtgag ttgttattcc ctatagtgag tcgtattacc
     5401 acccaacttt tctatacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag
     5461 agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc
     5521 gtaccggtta gtaatgagat ccctcccccc cccctaacgt tactggccga agccgcttgg
     5581 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     5641 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     5701 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     5761 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     5821 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     5881 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     5941 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     6001 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     6061 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacacat
     6121 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     6181 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     6241 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     6301 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     6361 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     6421 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     6481 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     6541 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     6601 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     6661 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctaa
     6721 atcgatagat cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6781 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6841 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6901 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6961 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7021 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7081 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7141 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7201 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7261 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7321 gcctgagatc ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7381 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     7441 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7501 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7561 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7621 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7681 tgaatatcag agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag
     7741 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     7801 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7861 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7921 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     7981 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8041 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8101 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8161 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     8221 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8281 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8341 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8401 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8461 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8521 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8581 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8641 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8701 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8761 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8821 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8881 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8941 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9001 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9061 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9121 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9181 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     9241 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     9301 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     9361 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     9421 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     9481 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     9541 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9601 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9661 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9721 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     9781 gattcattaa tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag
     9841 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9901 ttttgcaaaa agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9961 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
    10021 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
    10081 tgacatgatt acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    10141 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
    10201 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
    10261 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
    10321 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
    10381 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
    10441 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
    10501 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
    10561 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
    10621 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
    10681 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
    10741 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
    10801 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
    10861 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10921 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    10981 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    11041 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    11101 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
    11161 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    11221 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    11281 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    11341 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
    11401 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
    11461 acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg
    11521 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
    11581 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
    11641 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
    11701 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
    11761 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
    11821 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
    11881 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
    11941 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
    12001 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
    12061 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
    12121 taacataa
//
